TY - JOUR
T1 - Involvement of the response regulator CtrA in the extracellular DNA production of the marine bacterium Rhodovulum sulfidophilum
AU - Komatsu, Hiroyuki
AU - Yamamoto, Junya
AU - Suzuki, Hiromichi
AU - Nagao, Nobuyoshi
AU - Hirose, Yuu
AU - Ohyama, Takashi
AU - Umekage, So
AU - Kikuchi, Yo
N1 - Funding Information:
This work was supported in part by a grant-in-aid for Scientific Research to Y. Kikuchi (Grant-in Aid for Scientific Research No. 25252017) from the Japan Society of the Promotion of Science (JSPS), and by the Institute for Fermentation, Osaka, to S. Umekage. We thank Professor Akira Hiraishi of Toyohashi University of Technology for comments about the experiment.
Publisher Copyright:
© 2018 Applied Microbiology, Molecular and Cellular Biosciences Research Foundation.
PY - 2018
Y1 - 2018
N2 - The marine bacterium Rhodovulum sulfidophilum is a nonsulfur phototrophic bacterium, which is known to produce extracellular nucleic acids in soluble form in culture medium. In the present paper, constructing the response regulator ctrAdeficient mutant of R. sulfidophilum, we found that this mutation causes a significant decrease in the extracellular DNA production. However, by the introduction of a plasmid containing the wild type ctrA gene into the mutant, the amount of extracellular DNA produced was recovered. This is the first and clear evidence that the extracellular DNA production is actively controlled by the CtrA in R. sulfidophilum.
AB - The marine bacterium Rhodovulum sulfidophilum is a nonsulfur phototrophic bacterium, which is known to produce extracellular nucleic acids in soluble form in culture medium. In the present paper, constructing the response regulator ctrAdeficient mutant of R. sulfidophilum, we found that this mutation causes a significant decrease in the extracellular DNA production. However, by the introduction of a plasmid containing the wild type ctrA gene into the mutant, the amount of extracellular DNA produced was recovered. This is the first and clear evidence that the extracellular DNA production is actively controlled by the CtrA in R. sulfidophilum.
KW - CtrA
KW - Extracellular DNA
KW - Gene transfer agent
KW - Marine phototrophic bacterium
KW - Rhodovulum sulfidophilum
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U2 - 10.2323/jgam.2017.08.003
DO - 10.2323/jgam.2017.08.003
M3 - Article
C2 - 29526925
AN - SCOPUS:85050927753
SN - 0022-1260
VL - 64
SP - 103
EP - 107
JO - Journal of General and Applied Microbiology
JF - Journal of General and Applied Microbiology
IS - 3
ER -
