Involvement of the response regulator CtrA in the extracellular DNA production of the marine bacterium Rhodovulum sulfidophilum

Hiroyuki Komatsu, Junya Yamamoto, Hiromichi Suzuki, Nobuyoshi Nagao, Yuu Hirose, Takashi Ohyama, So Umekage, Yo Kikuchi

    Research output: Contribution to journalArticle

    1 Citation (Scopus)

    Abstract

    The marine bacterium Rhodovulum sulfidophilum is a nonsulfur phototrophic bacterium, which is known to produce extracellular nucleic acids in soluble form in culture medium. In the present paper, constructing the response regulator ctrAdeficient mutant of R. sulfidophilum, we found that this mutation causes a significant decrease in the extracellular DNA production. However, by the introduction of a plasmid containing the wild type ctrA gene into the mutant, the amount of extracellular DNA produced was recovered. This is the first and clear evidence that the extracellular DNA production is actively controlled by the CtrA in R. sulfidophilum.

    Original languageEnglish
    Pages (from-to)103-107
    Number of pages5
    JournalJournal of General and Applied Microbiology
    Volume64
    Issue number3
    DOIs
    Publication statusPublished - 2018 Jan 1

    Fingerprint

    Rhodovulum
    Bacteria
    DNA
    Nucleic Acids
    Culture Media
    Plasmids
    Mutation
    Genes

    Keywords

    • CtrA
    • Extracellular DNA
    • Gene transfer agent
    • Marine phototrophic bacterium
    • Rhodovulum sulfidophilum

    ASJC Scopus subject areas

    • Microbiology
    • Applied Microbiology and Biotechnology

    Cite this

    Involvement of the response regulator CtrA in the extracellular DNA production of the marine bacterium Rhodovulum sulfidophilum. / Komatsu, Hiroyuki; Yamamoto, Junya; Suzuki, Hiromichi; Nagao, Nobuyoshi; Hirose, Yuu; Ohyama, Takashi; Umekage, So; Kikuchi, Yo.

    In: Journal of General and Applied Microbiology, Vol. 64, No. 3, 01.01.2018, p. 103-107.

    Research output: Contribution to journalArticle

    Komatsu, Hiroyuki ; Yamamoto, Junya ; Suzuki, Hiromichi ; Nagao, Nobuyoshi ; Hirose, Yuu ; Ohyama, Takashi ; Umekage, So ; Kikuchi, Yo. / Involvement of the response regulator CtrA in the extracellular DNA production of the marine bacterium Rhodovulum sulfidophilum. In: Journal of General and Applied Microbiology. 2018 ; Vol. 64, No. 3. pp. 103-107.
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    AU - Hirose, Yuu

    AU - Ohyama, Takashi

    AU - Umekage, So

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