Ion exchange of lysozyme during permeation across a microporous sulfopropyl-group-containing hollow fiber

Hironori Shinano, Satoshi Tsuneda, Kyoichi Saito, Shintaro Furusaki, Takanobu Sugo

Research output: Contribution to journalArticle

48 Citations (Scopus)

Abstract

A microporous hollow fiber containing a sulfopropyl (SP) group as a strongly acidic cation-exchange group was prepared by radiation-induced graft polymerization of glycidyl methacrylate, followed by hydrolysis of the resulting epoxide group into a diol, and then conversion of the diol into the SP group. The SP group density of the resulting hollow fiber ranged from 0.21 to 0.84 mol/kg of dry fiber with a pure water flux of 2.7 m/h at a filtration pressure of 0.1 MPa. Lysozyme adsorption was examined during permeation of the lysozyme solution (pH 6) through the pores across a microporous cation-exchange hollow fiber. The lysozyme concentration of the effluent penetrating the outside of the hollow fiber did not change irrespective of the residence time of the solution across the hollow fiber, which was indicative of the negligible diffusional resistance of lysozyme to the SP group. The binding capacity of lysozyme to the fiber was constant in this range of SP group density. For comparison, the adsorption characteristics of a cupric chloride solution during permeation were also determined. The binding capacity of Cu to the fiber increased linearly with increasing SP group density, because cupric ions of a smaller size than lysozyme can invade the depths of the grafted polymer branches formed in the amorphous domain of the polymer matrix.

Original languageEnglish
Pages (from-to)193-198
Number of pages6
JournalBiotechnology Progress
Volume9
Issue number2
Publication statusPublished - 1993 Mar
Externally publishedYes

Fingerprint

Ion Exchange
ion exchange
Muramidase
lysozyme
glycols
cation exchange
binding capacity
Adsorption
Cations
polymers
Polymers
adsorption
Epoxy Compounds
epoxides
Polymerization
polymerization
effluents
chlorides
Hydrolysis
hydrolysis

ASJC Scopus subject areas

  • Food Science
  • Biotechnology
  • Microbiology

Cite this

Ion exchange of lysozyme during permeation across a microporous sulfopropyl-group-containing hollow fiber. / Shinano, Hironori; Tsuneda, Satoshi; Saito, Kyoichi; Furusaki, Shintaro; Sugo, Takanobu.

In: Biotechnology Progress, Vol. 9, No. 2, 03.1993, p. 193-198.

Research output: Contribution to journalArticle

Shinano, Hironori ; Tsuneda, Satoshi ; Saito, Kyoichi ; Furusaki, Shintaro ; Sugo, Takanobu. / Ion exchange of lysozyme during permeation across a microporous sulfopropyl-group-containing hollow fiber. In: Biotechnology Progress. 1993 ; Vol. 9, No. 2. pp. 193-198.
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