Abstract
Domestic ducks were chronically equipped with a device probing the third cerebral ventricle (VIII) for localized intracerebroventricular (i.c.v.) perfusion. In conscious animals made diuretic by intravenous water loading with 1.0 ml/min hypoosmotic glucose solution (200 mOsm/kg), hyperosmotic i.c.v. stimulations were tested for antidiuretic actions. Artificial cerebrospinal fluid made hypertonic (400 mOsm/kg) by adding sucrose, mannitol, NaCl, LiCl, choline chloride, NaI, NaNO3, LiNO3, CaCl2 or MgCl2 was perfused i.c.v. for 10-15 min at rates of 10-15 μl/min. Arterial pressure and heart rate were monitored continuously. Hyperosmotic stimulations with non-electrolytes did not induce antidiuresis. Approximately equivalent degrees of antidiuresis were elicited by Na+-, Li+- and choline salts with a tendency for moderate rises in arterial pressure. Compared to Cl-- and I--salts, the effects of NO3 --salts were attenuated. Divalent cations caused prolonged antidiuresis, sometimes preceded by initial diuresis, with circulatory side effects unrelated to the changes in renal fluid excretion. It is concluded that the observed antidiuretic effects were mediated by cation-sensitive, rather than osmosensitive neurons on the brain side of the blood-brain-barrier. Their transduction mechanism might consist of poorly selective membrane channels permeable to cations but not to anions.
| Original language | English |
|---|---|
| Pages (from-to) | 268-274 |
| Number of pages | 7 |
| Journal | Brain Research |
| Volume | 569 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - 1992 Jan 13 |
| Externally published | Yes |
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Keywords
- Anterior third ventricle
- Avian osmoregulation
- Cerebral osmoreception
- Sodium sensor
- Urine formation
ASJC Scopus subject areas
- Developmental Biology
- Molecular Biology
- Clinical Neurology
- Neuroscience(all)
Cite this
Ionic responsiveness in third ventricular hypertonic stimulation of antidiuresis in ducks. / Kanosue, Kazuyuki; Gerstberger, Rüdiger; Simon-Oppermann, Christa; Simon, Eckhart.
In: Brain Research, Vol. 569, No. 2, 13.01.1992, p. 268-274.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Ionic responsiveness in third ventricular hypertonic stimulation of antidiuresis in ducks
AU - Kanosue, Kazuyuki
AU - Gerstberger, Rüdiger
AU - Simon-Oppermann, Christa
AU - Simon, Eckhart
PY - 1992/1/13
Y1 - 1992/1/13
N2 - Domestic ducks were chronically equipped with a device probing the third cerebral ventricle (VIII) for localized intracerebroventricular (i.c.v.) perfusion. In conscious animals made diuretic by intravenous water loading with 1.0 ml/min hypoosmotic glucose solution (200 mOsm/kg), hyperosmotic i.c.v. stimulations were tested for antidiuretic actions. Artificial cerebrospinal fluid made hypertonic (400 mOsm/kg) by adding sucrose, mannitol, NaCl, LiCl, choline chloride, NaI, NaNO3, LiNO3, CaCl2 or MgCl2 was perfused i.c.v. for 10-15 min at rates of 10-15 μl/min. Arterial pressure and heart rate were monitored continuously. Hyperosmotic stimulations with non-electrolytes did not induce antidiuresis. Approximately equivalent degrees of antidiuresis were elicited by Na+-, Li+- and choline salts with a tendency for moderate rises in arterial pressure. Compared to Cl-- and I--salts, the effects of NO3 --salts were attenuated. Divalent cations caused prolonged antidiuresis, sometimes preceded by initial diuresis, with circulatory side effects unrelated to the changes in renal fluid excretion. It is concluded that the observed antidiuretic effects were mediated by cation-sensitive, rather than osmosensitive neurons on the brain side of the blood-brain-barrier. Their transduction mechanism might consist of poorly selective membrane channels permeable to cations but not to anions.
AB - Domestic ducks were chronically equipped with a device probing the third cerebral ventricle (VIII) for localized intracerebroventricular (i.c.v.) perfusion. In conscious animals made diuretic by intravenous water loading with 1.0 ml/min hypoosmotic glucose solution (200 mOsm/kg), hyperosmotic i.c.v. stimulations were tested for antidiuretic actions. Artificial cerebrospinal fluid made hypertonic (400 mOsm/kg) by adding sucrose, mannitol, NaCl, LiCl, choline chloride, NaI, NaNO3, LiNO3, CaCl2 or MgCl2 was perfused i.c.v. for 10-15 min at rates of 10-15 μl/min. Arterial pressure and heart rate were monitored continuously. Hyperosmotic stimulations with non-electrolytes did not induce antidiuresis. Approximately equivalent degrees of antidiuresis were elicited by Na+-, Li+- and choline salts with a tendency for moderate rises in arterial pressure. Compared to Cl-- and I--salts, the effects of NO3 --salts were attenuated. Divalent cations caused prolonged antidiuresis, sometimes preceded by initial diuresis, with circulatory side effects unrelated to the changes in renal fluid excretion. It is concluded that the observed antidiuretic effects were mediated by cation-sensitive, rather than osmosensitive neurons on the brain side of the blood-brain-barrier. Their transduction mechanism might consist of poorly selective membrane channels permeable to cations but not to anions.
KW - Anterior third ventricle
KW - Avian osmoregulation
KW - Cerebral osmoreception
KW - Sodium sensor
KW - Urine formation
UR - http://www.scopus.com/inward/record.url?scp=0026542601&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0026542601&partnerID=8YFLogxK
U2 - 10.1016/0006-8993(92)90638-P
DO - 10.1016/0006-8993(92)90638-P
M3 - Article
C2 - 1311617
AN - SCOPUS:0026542601
VL - 569
SP - 268
EP - 274
JO - Brain Research
JF - Brain Research
SN - 0006-8993
IS - 2
ER -