Iron feeding optimization and plasmid stability in production of recombinant bacterial magnetic particles by Magnetospirillum magneticum AMB-1 in fed-batch culture

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

The production of bacterial magnetic particles (BMPs) by recombinant Magnetospirillum magneticum AMB-1 harboring the plasmid pKML was enhanced in pH-regulated fed-batch culture. The addition of fresh nutrients was feedback-controlled as a function of the pH of the culture. The yield of BMPs was optimized by adjusting the rate of ferric iron addition. Feeding ferric quinate at 15.4 μg/min resulted in a BMP yield of 7.5 mg/l, which is the highest yield so far reported. Expression of a plasmid-encoded fusion protein and segregation of the plasmid during bacterial growth were both stable during fed-batch culture. More than 75% of the cells retained the plasmid for 130 h under antibiotic-free conditions. In addition, the fusion protein permitting the display of a specific protein on the BMP surface was also stably expressed.

Original languageEnglish
Pages (from-to)213-216
Number of pages4
JournalJournal of Bioscience and Bioengineering
Volume91
Issue number2
DOIs
Publication statusPublished - 2001
Externally publishedYes

Fingerprint

Magnetospirillum
Batch Cell Culture Techniques
Plasmids
Iron
Proteins
Fusion reactions
Quinic Acid
Antibiotics
Nutrients
Bacterial Proteins
Display devices
Feedback
Anti-Bacterial Agents
Food
Growth

Keywords

  • Bacterial magnetic particles (BMPs)
  • BMP production
  • Fed-batch culture
  • Iron source
  • Magnetospirillum magneticum AMB-1
  • Plasmid stability

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering

Cite this

@article{6f04bd9d49254baf87af016e6dbbef09,
title = "Iron feeding optimization and plasmid stability in production of recombinant bacterial magnetic particles by Magnetospirillum magneticum AMB-1 in fed-batch culture",
abstract = "The production of bacterial magnetic particles (BMPs) by recombinant Magnetospirillum magneticum AMB-1 harboring the plasmid pKML was enhanced in pH-regulated fed-batch culture. The addition of fresh nutrients was feedback-controlled as a function of the pH of the culture. The yield of BMPs was optimized by adjusting the rate of ferric iron addition. Feeding ferric quinate at 15.4 μg/min resulted in a BMP yield of 7.5 mg/l, which is the highest yield so far reported. Expression of a plasmid-encoded fusion protein and segregation of the plasmid during bacterial growth were both stable during fed-batch culture. More than 75{\%} of the cells retained the plasmid for 130 h under antibiotic-free conditions. In addition, the fusion protein permitting the display of a specific protein on the BMP surface was also stably expressed.",
keywords = "Bacterial magnetic particles (BMPs), BMP production, Fed-batch culture, Iron source, Magnetospirillum magneticum AMB-1, Plasmid stability",
author = "Chendong Yang and Haruko Takeyama and Tadashi Matsunaga",
year = "2001",
doi = "10.1016/S1389-1723(01)80068-2",
language = "English",
volume = "91",
pages = "213--216",
journal = "Journal of Bioscience and Bioengineering",
issn = "1389-1723",
publisher = "Elsevier",
number = "2",

}

TY - JOUR

T1 - Iron feeding optimization and plasmid stability in production of recombinant bacterial magnetic particles by Magnetospirillum magneticum AMB-1 in fed-batch culture

AU - Yang, Chendong

AU - Takeyama, Haruko

AU - Matsunaga, Tadashi

PY - 2001

Y1 - 2001

N2 - The production of bacterial magnetic particles (BMPs) by recombinant Magnetospirillum magneticum AMB-1 harboring the plasmid pKML was enhanced in pH-regulated fed-batch culture. The addition of fresh nutrients was feedback-controlled as a function of the pH of the culture. The yield of BMPs was optimized by adjusting the rate of ferric iron addition. Feeding ferric quinate at 15.4 μg/min resulted in a BMP yield of 7.5 mg/l, which is the highest yield so far reported. Expression of a plasmid-encoded fusion protein and segregation of the plasmid during bacterial growth were both stable during fed-batch culture. More than 75% of the cells retained the plasmid for 130 h under antibiotic-free conditions. In addition, the fusion protein permitting the display of a specific protein on the BMP surface was also stably expressed.

AB - The production of bacterial magnetic particles (BMPs) by recombinant Magnetospirillum magneticum AMB-1 harboring the plasmid pKML was enhanced in pH-regulated fed-batch culture. The addition of fresh nutrients was feedback-controlled as a function of the pH of the culture. The yield of BMPs was optimized by adjusting the rate of ferric iron addition. Feeding ferric quinate at 15.4 μg/min resulted in a BMP yield of 7.5 mg/l, which is the highest yield so far reported. Expression of a plasmid-encoded fusion protein and segregation of the plasmid during bacterial growth were both stable during fed-batch culture. More than 75% of the cells retained the plasmid for 130 h under antibiotic-free conditions. In addition, the fusion protein permitting the display of a specific protein on the BMP surface was also stably expressed.

KW - Bacterial magnetic particles (BMPs)

KW - BMP production

KW - Fed-batch culture

KW - Iron source

KW - Magnetospirillum magneticum AMB-1

KW - Plasmid stability

UR - http://www.scopus.com/inward/record.url?scp=0035101992&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0035101992&partnerID=8YFLogxK

U2 - 10.1016/S1389-1723(01)80068-2

DO - 10.1016/S1389-1723(01)80068-2

M3 - Article

VL - 91

SP - 213

EP - 216

JO - Journal of Bioscience and Bioengineering

JF - Journal of Bioscience and Bioengineering

SN - 1389-1723

IS - 2

ER -