Iron oxide crystal formation on a substrate modified with the Mms6 protein from magnetotactic bacteria

Atsushi Arakaki, Fukashi Masuda, Tadashi Matsunaga

Research output: Chapter in Book/Report/Conference proceedingConference contribution

5 Citations (Scopus)

Abstract

Mms6 is a small acidic protein which is tightly bound to magnetite in the bacterium Magnetospirillum magneticum AMB-1. Mms6 has been previously shown to promote iron-binding capacity as well as modulate the size and morphology of magnetic iron oxide crystals in vitro. In this study, we synthesized iron oxide crystals by using a monolayer-modified substrate. A self-assembled monolayer of octadecyltrimethoxysilane was modified on a silicon substrate. Recombinant Mms6 protein was attached to the substrate through the hydrophobic interactions between the protein molecules and the monolayer. The immobilization of protein molecules on the substrate surface was confirmed by fluorescent labeling of these molecules and subsequent fluorescence microscopy. This protein-modified substrate was then used as a template for iron oxide crystal formation in a ferrous solution. Scanning electron microscopy revealed site-specific formation of iron oxide crystals in substrate regions with immobilized proteins. This use of proteins might provide an alternative method for the bottom-up fabrication of nano-sized magnetic particles.

Original languageEnglish
Title of host publicationStructure-Property Relationships in Biomineralized and Biomimetic Composites
Pages49-53
Number of pages5
Volume1187
Publication statusPublished - 2009
Externally publishedYes
Event2009 MRS Spring Meeting - San Francisco, CA, United States
Duration: 2009 Apr 142009 Apr 17

Other

Other2009 MRS Spring Meeting
CountryUnited States
CitySan Francisco, CA
Period09/4/1409/4/17

Fingerprint

Iron oxides
iron oxides
bacteria
Bacteria
proteins
Proteins
Crystals
Substrates
crystals
Molecules
Monolayers
Immobilized Proteins
Ferrosoferric Oxide
Recombinant proteins
Fluorescence microscopy
molecules
Self assembled monolayers
Magnetite
Silicon
Recombinant Proteins

ASJC Scopus subject areas

  • Materials Science(all)
  • Condensed Matter Physics
  • Mechanics of Materials
  • Mechanical Engineering

Cite this

Arakaki, A., Masuda, F., & Matsunaga, T. (2009). Iron oxide crystal formation on a substrate modified with the Mms6 protein from magnetotactic bacteria. In Structure-Property Relationships in Biomineralized and Biomimetic Composites (Vol. 1187, pp. 49-53)

Iron oxide crystal formation on a substrate modified with the Mms6 protein from magnetotactic bacteria. / Arakaki, Atsushi; Masuda, Fukashi; Matsunaga, Tadashi.

Structure-Property Relationships in Biomineralized and Biomimetic Composites. Vol. 1187 2009. p. 49-53.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Arakaki, A, Masuda, F & Matsunaga, T 2009, Iron oxide crystal formation on a substrate modified with the Mms6 protein from magnetotactic bacteria. in Structure-Property Relationships in Biomineralized and Biomimetic Composites. vol. 1187, pp. 49-53, 2009 MRS Spring Meeting, San Francisco, CA, United States, 09/4/14.
Arakaki A, Masuda F, Matsunaga T. Iron oxide crystal formation on a substrate modified with the Mms6 protein from magnetotactic bacteria. In Structure-Property Relationships in Biomineralized and Biomimetic Composites. Vol. 1187. 2009. p. 49-53
Arakaki, Atsushi ; Masuda, Fukashi ; Matsunaga, Tadashi. / Iron oxide crystal formation on a substrate modified with the Mms6 protein from magnetotactic bacteria. Structure-Property Relationships in Biomineralized and Biomimetic Composites. Vol. 1187 2009. pp. 49-53
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