Iron-regulated expression and membrane localization of the maga protein in magnetospirillum sp. strain AMB-1

Chikashi Nakamura, Tomoko Kikuchi, J. Grant Burgess, Tadashi Matsunaga

Research output: Contribution to journalArticle

75 Citations (Scopus)

Abstract

The magA gene from Magnetospirillum sp. strain AMB-1 is required for the synthesis of bacterial magnetic particles (BMPs). This gene has been cloned, sequenced and found to encode a protein which is homologous to the Escherichia coli potassium efflux membrane-binding protein, KefC. By using the firefly luciferase gene (luc) cloned downstream of the magA promoter, the effect of iron on regulation of magA expression was investigated, and transcription of magA was found to be enhanced by low concentrations of iron. Intracellular localization of the MagA protein was studied using magA-luc fusion proteins. The luc gene was cloned downstream of the magA hydrophilic C-terminal domain. Detection of luciferase activity in the cytoplasm, cell membrane, and magnetic particle membrane subcellular fractions confirmed that the MagA fusion protein was localized in the cell membrane. The fusion protein was also detected on the surface of the lipid bilayer covering the magnetic particles. These results suggest that MagA is a membrane-bound protein, the expression of which is enhanced at low iron concentrations.

Original languageEnglish
Pages (from-to)23-27
Number of pages5
JournalJournal of Biochemistry
Volume118
Issue number1
Publication statusPublished - 1995 Jul
Externally publishedYes

Fingerprint

Iron
Membrane
Proteins
Membranes
Protein
Genes
Fusion reactions
Gene
Fusion
Cell membranes
Magnetospirillum
Membrane Proteins
Cell Membrane
Firefly Luciferases
Subcellular Fractions
Lipid Bilayers
Lipid bilayers
Luciferases
Lipid Bilayer
Transcription

Keywords

  • Biomineralization
  • Gene fusion
  • Magnetic particle
  • Magnetospirillum
  • Protein localization

ASJC Scopus subject areas

  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Physiology (medical)
  • Radiology Nuclear Medicine and imaging
  • Molecular Biology
  • Biochemistry

Cite this

Iron-regulated expression and membrane localization of the maga protein in magnetospirillum sp. strain AMB-1. / Nakamura, Chikashi; Kikuchi, Tomoko; Burgess, J. Grant; Matsunaga, Tadashi.

In: Journal of Biochemistry, Vol. 118, No. 1, 07.1995, p. 23-27.

Research output: Contribution to journalArticle

Nakamura, Chikashi ; Kikuchi, Tomoko ; Burgess, J. Grant ; Matsunaga, Tadashi. / Iron-regulated expression and membrane localization of the maga protein in magnetospirillum sp. strain AMB-1. In: Journal of Biochemistry. 1995 ; Vol. 118, No. 1. pp. 23-27.
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