Islet1 selectively promotes peripheral axon outgrowth in Rohon-Beard primary sensory neurons

Hideomi Tanaka, Yasuhiro Nojima, Wataru Shoji, Miki Sato, Ryoko Nakayama, Toshio Ohshima, Hitoshi Okamoto

    Research output: Contribution to journalArticle

    19 Citations (Scopus)

    Abstract

    We isolated a novel zebrafish mutant, lullaby (llb), and showed that the llb locus encodes the zebrafish orthologue of isl1. Rohon-Beard (RB) primary sensory neurons are multipolar neurons that extend their central axons longitudinally within the spinal cord and also extend their peripheral axons under the skin. In llb embryos, the outgrowth of the peripheral axons of RB neurons was selectively impaired, which correlated with down-regulation of the expression of dihydropyrimidinase-like 3 (dpysl3, also known as collapsin response mediator protein 4, crmp4). Antisense morpholino oligonucleotide (AMO)-mediated knockdown of dpysl3 inhibited the outgrowth of the peripheral axons of RB neurons, and semaphorin 3d (sema3d) AMO enhanced this effect. These data indicate that Dpysl3 is cooperating with Sema3d in the peripheral axon outgrowth, and Isl1 is required for the selective outgrowth of the peripheral axons of RB neurons by maintaining the expression of dpysl3.

    Original languageEnglish
    Pages (from-to)9-22
    Number of pages14
    JournalDevelopmental Dynamics
    Volume240
    Issue number1
    DOIs
    Publication statusPublished - 2011 Jan

    Fingerprint

    Sensory Receptor Cells
    Neurons
    Morpholinos
    Antisense Oligonucleotides
    Zebrafish
    dihydropyrimidinase
    Axons
    Semaphorin-3A
    Semaphorins
    Spinal Cord
    Down-Regulation
    Embryonic Structures
    Skin
    Neuronal Outgrowth
    Proteins

    Keywords

    • Axon guidance
    • CRMP4
    • Dpysl3
    • Islet1
    • Rohon-Beard neuron
    • Semaphorin
    • Zebrafish

    ASJC Scopus subject areas

    • Developmental Biology

    Cite this

    Islet1 selectively promotes peripheral axon outgrowth in Rohon-Beard primary sensory neurons. / Tanaka, Hideomi; Nojima, Yasuhiro; Shoji, Wataru; Sato, Miki; Nakayama, Ryoko; Ohshima, Toshio; Okamoto, Hitoshi.

    In: Developmental Dynamics, Vol. 240, No. 1, 01.2011, p. 9-22.

    Research output: Contribution to journalArticle

    Tanaka, Hideomi ; Nojima, Yasuhiro ; Shoji, Wataru ; Sato, Miki ; Nakayama, Ryoko ; Ohshima, Toshio ; Okamoto, Hitoshi. / Islet1 selectively promotes peripheral axon outgrowth in Rohon-Beard primary sensory neurons. In: Developmental Dynamics. 2011 ; Vol. 240, No. 1. pp. 9-22.
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