Islet1 selectively promotes peripheral axon outgrowth in Rohon-Beard primary sensory neurons

Hideomi Tanaka, Yasuhiro Nojima, Wataru Shoji, Miki Sato, Ryoko Nakayama, Toshio Ohshima, Hitoshi Okamoto

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

We isolated a novel zebrafish mutant, lullaby (llb), and showed that the llb locus encodes the zebrafish orthologue of isl1. Rohon-Beard (RB) primary sensory neurons are multipolar neurons that extend their central axons longitudinally within the spinal cord and also extend their peripheral axons under the skin. In llb embryos, the outgrowth of the peripheral axons of RB neurons was selectively impaired, which correlated with down-regulation of the expression of dihydropyrimidinase-like 3 (dpysl3, also known as collapsin response mediator protein 4, crmp4). Antisense morpholino oligonucleotide (AMO)-mediated knockdown of dpysl3 inhibited the outgrowth of the peripheral axons of RB neurons, and semaphorin 3d (sema3d) AMO enhanced this effect. These data indicate that Dpysl3 is cooperating with Sema3d in the peripheral axon outgrowth, and Isl1 is required for the selective outgrowth of the peripheral axons of RB neurons by maintaining the expression of dpysl3.

Original languageEnglish
Pages (from-to)9-22
Number of pages14
JournalDevelopmental Dynamics
Volume240
Issue number1
DOIs
Publication statusPublished - 2011 Jan 1

Keywords

  • Axon guidance
  • CRMP4
  • Dpysl3
  • Islet1
  • Rohon-Beard neuron
  • Semaphorin
  • Zebrafish

ASJC Scopus subject areas

  • Developmental Biology

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