Isolation and Characterization of Two Forms of Xenopus Prolactin

Kaoru Yamashita, Kouhei Matsuda, Hiroaki Hayashi, Yoichi Hanaoka, Shigeyasu Tanaka, Kazutoshi Yamamoto, Sakaé Kikuyama

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    9 Citations (Scopus)

    Abstract

    Two forms of highly purified prolactin (PRL) were obtained from pituitary glands of Xenopus laevis by extraction of acetone-dried powder with acid acetone and high-performance liquid chromatography on anion exchange, gel filtration, and reverse-phase columns. Purification was monitored by SDS-polyacrylamide gel electrophoresis (SDS-PAGE) and Western blot analysis employing antiserum against bullfrog PRL. The Xenopus prolactins (xPRL-I and xPRL-II) thus obtained were shown to have similar molecular weights of 23,000 as determined by SDS-PAGE. The isoelectric points of xPRL-I and xPRL-II determined by isoelectric focusing were 5.6 and 5.3, respectively. Both hormones blocked T4-induced shrinkage of Xenopus tadpole tail fin in vitro. The amino acid compositions of the xPRLs resembled that of bullfrog PRL. The partial amino acid sequences of xPRL-I and of xPRL-II showed 78 and 68% homology with the comparable portion of the sequence of bullfrog PRL, respectively. Homology between xPRL-I and xPRL-II was 90%.

    Original languageEnglish
    Pages (from-to)307-317
    Number of pages11
    JournalGeneral and Comparative Endocrinology
    Volume91
    Issue number3
    DOIs
    Publication statusPublished - 1993 Sep

    ASJC Scopus subject areas

    • Endocrinology

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  • Cite this

    Yamashita, K., Matsuda, K., Hayashi, H., Hanaoka, Y., Tanaka, S., Yamamoto, K., & Kikuyama, S. (1993). Isolation and Characterization of Two Forms of Xenopus Prolactin. General and Comparative Endocrinology, 91(3), 307-317. https://doi.org/10.1006/gcen.1993.1131