Isolation and sequencing of cDNA clones homologous to the v-fgr oncogene from a human B lymphocyte cell line, IM-9

K. Inoue, S. Ikawa, Kentaro Senba, J. Sukegawa, T. Yamamoto, K. Toyoshima

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Two c-fgr cDNA clones were isolated from a cDNA library derived from a human B lymphocyte cell line, IM-9. Sequence analysis of the clones showed that they contained inserts corresponding to nearly full-length human c-fgr mRNA, which could encode a polypeptide of 529 amino acids with a calculated molecular weight of 59478. Although the amino acid sequence between Gly-78 and the carboxy-terminus of the c-fgr is highly homologous to the corresponding sequence of the c-yes protein, the homology between the two proteins is low in the amino-terminal proximal region. Northern blot hybridization analysis using the c-fgr specific sequence showed that the c-fgr mRNA was expressed at higher level in the liver than in the brain, lung, or kidney of a human fetus.

Original languageEnglish
Pages (from-to)301-304
Number of pages4
JournalOncogene
Volume1
Issue number3
Publication statusPublished - 1987
Externally publishedYes

Fingerprint

Oncogenes
B-Lymphocytes
Complementary DNA
Clone Cells
Proto-Oncogene Proteins c-yes
Cell Line
Messenger RNA
Gene Library
Northern Blotting
Sequence Analysis
Amino Acid Sequence
Fetus
Molecular Weight
Kidney
Amino Acids
Lung
Peptides
Liver
Brain
Proteins

ASJC Scopus subject areas

  • Cancer Research
  • Genetics
  • Molecular Biology

Cite this

Inoue, K., Ikawa, S., Senba, K., Sukegawa, J., Yamamoto, T., & Toyoshima, K. (1987). Isolation and sequencing of cDNA clones homologous to the v-fgr oncogene from a human B lymphocyte cell line, IM-9. Oncogene, 1(3), 301-304.

Isolation and sequencing of cDNA clones homologous to the v-fgr oncogene from a human B lymphocyte cell line, IM-9. / Inoue, K.; Ikawa, S.; Senba, Kentaro; Sukegawa, J.; Yamamoto, T.; Toyoshima, K.

In: Oncogene, Vol. 1, No. 3, 1987, p. 301-304.

Research output: Contribution to journalArticle

Inoue, K, Ikawa, S, Senba, K, Sukegawa, J, Yamamoto, T & Toyoshima, K 1987, 'Isolation and sequencing of cDNA clones homologous to the v-fgr oncogene from a human B lymphocyte cell line, IM-9', Oncogene, vol. 1, no. 3, pp. 301-304.
Inoue, K. ; Ikawa, S. ; Senba, Kentaro ; Sukegawa, J. ; Yamamoto, T. ; Toyoshima, K. / Isolation and sequencing of cDNA clones homologous to the v-fgr oncogene from a human B lymphocyte cell line, IM-9. In: Oncogene. 1987 ; Vol. 1, No. 3. pp. 301-304.
@article{85adb9b5be3949d38feaf131602800d2,
title = "Isolation and sequencing of cDNA clones homologous to the v-fgr oncogene from a human B lymphocyte cell line, IM-9",
abstract = "Two c-fgr cDNA clones were isolated from a cDNA library derived from a human B lymphocyte cell line, IM-9. Sequence analysis of the clones showed that they contained inserts corresponding to nearly full-length human c-fgr mRNA, which could encode a polypeptide of 529 amino acids with a calculated molecular weight of 59478. Although the amino acid sequence between Gly-78 and the carboxy-terminus of the c-fgr is highly homologous to the corresponding sequence of the c-yes protein, the homology between the two proteins is low in the amino-terminal proximal region. Northern blot hybridization analysis using the c-fgr specific sequence showed that the c-fgr mRNA was expressed at higher level in the liver than in the brain, lung, or kidney of a human fetus.",
author = "K. Inoue and S. Ikawa and Kentaro Senba and J. Sukegawa and T. Yamamoto and K. Toyoshima",
year = "1987",
language = "English",
volume = "1",
pages = "301--304",
journal = "Oncogene",
issn = "0950-9232",
publisher = "Nature Publishing Group",
number = "3",

}

TY - JOUR

T1 - Isolation and sequencing of cDNA clones homologous to the v-fgr oncogene from a human B lymphocyte cell line, IM-9

AU - Inoue, K.

AU - Ikawa, S.

AU - Senba, Kentaro

AU - Sukegawa, J.

AU - Yamamoto, T.

AU - Toyoshima, K.

PY - 1987

Y1 - 1987

N2 - Two c-fgr cDNA clones were isolated from a cDNA library derived from a human B lymphocyte cell line, IM-9. Sequence analysis of the clones showed that they contained inserts corresponding to nearly full-length human c-fgr mRNA, which could encode a polypeptide of 529 amino acids with a calculated molecular weight of 59478. Although the amino acid sequence between Gly-78 and the carboxy-terminus of the c-fgr is highly homologous to the corresponding sequence of the c-yes protein, the homology between the two proteins is low in the amino-terminal proximal region. Northern blot hybridization analysis using the c-fgr specific sequence showed that the c-fgr mRNA was expressed at higher level in the liver than in the brain, lung, or kidney of a human fetus.

AB - Two c-fgr cDNA clones were isolated from a cDNA library derived from a human B lymphocyte cell line, IM-9. Sequence analysis of the clones showed that they contained inserts corresponding to nearly full-length human c-fgr mRNA, which could encode a polypeptide of 529 amino acids with a calculated molecular weight of 59478. Although the amino acid sequence between Gly-78 and the carboxy-terminus of the c-fgr is highly homologous to the corresponding sequence of the c-yes protein, the homology between the two proteins is low in the amino-terminal proximal region. Northern blot hybridization analysis using the c-fgr specific sequence showed that the c-fgr mRNA was expressed at higher level in the liver than in the brain, lung, or kidney of a human fetus.

UR - http://www.scopus.com/inward/record.url?scp=0023619371&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0023619371&partnerID=8YFLogxK

M3 - Article

C2 - 3330776

AN - SCOPUS:0023619371

VL - 1

SP - 301

EP - 304

JO - Oncogene

JF - Oncogene

SN - 0950-9232

IS - 3

ER -