Isolation of a cDNA Clone Encoding DNA-Binding Protein (TAXREB107) That Binds Specifically to Domain C of the tax-Responsive Enhancer Element in the Long Terminal Repeat of Human T-Cell Leukemia Virus Type I

Toshiro Morita, Takako Sato, Hiroshi Nyunoya, Atsumi Tsujimoto, Jiro Takahara, Shozo Irino, Kunitada Shimotohno

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Five cDNA clones for TAXREB proteins that bind to the tax-responsive enhancer element of human T-cell leukemia virus type I (HTLV-I) were isolated from a Jurkat cell cDNA library. The β-galactosidase fusion proteins of three of these clones specifically recognized the domain C within the enhancer. One of the three cDNAs, encoding TAXREB107, contained an open reading frame with 288 amino acid residues. RNA blot analysis showed that the level of mRNA for TAXREB107 increased transiently in Jurkat cells on treatment with TPA. Immunoblot analysis showed that polyclonal antibody against TAXREB107 specifically recognized a 34-kD protein in Jurkat cells. TAXREB107 may participate in tax-mediated trans-activation of transcription.

Original languageEnglish
Pages (from-to)115-121
Number of pages7
JournalAIDS Research and Human Retroviruses
Volume9
Issue number2
DOIs
Publication statusPublished - 1993
Externally publishedYes

Fingerprint

Human T-lymphotropic virus 1
Terminal Repeat Sequences
DNA-Binding Proteins
Jurkat Cells
Complementary DNA
Clone Cells
Galactosidases
Proteins
Gene Library
Transcriptional Activation
Open Reading Frames
RNA
Amino Acids
Messenger RNA
mouse tax-responsive-element-binding protein 107
Antibodies

ASJC Scopus subject areas

  • Immunology
  • Virology
  • Infectious Diseases

Cite this

Isolation of a cDNA Clone Encoding DNA-Binding Protein (TAXREB107) That Binds Specifically to Domain C of the tax-Responsive Enhancer Element in the Long Terminal Repeat of Human T-Cell Leukemia Virus Type I. / Morita, Toshiro; Sato, Takako; Nyunoya, Hiroshi; Tsujimoto, Atsumi; Takahara, Jiro; Irino, Shozo; Shimotohno, Kunitada.

In: AIDS Research and Human Retroviruses, Vol. 9, No. 2, 1993, p. 115-121.

Research output: Contribution to journalArticle

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abstract = "Five cDNA clones for TAXREB proteins that bind to the tax-responsive enhancer element of human T-cell leukemia virus type I (HTLV-I) were isolated from a Jurkat cell cDNA library. The β-galactosidase fusion proteins of three of these clones specifically recognized the domain C within the enhancer. One of the three cDNAs, encoding TAXREB107, contained an open reading frame with 288 amino acid residues. RNA blot analysis showed that the level of mRNA for TAXREB107 increased transiently in Jurkat cells on treatment with TPA. Immunoblot analysis showed that polyclonal antibody against TAXREB107 specifically recognized a 34-kD protein in Jurkat cells. TAXREB107 may participate in tax-mediated trans-activation of transcription.",
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AU - Morita, Toshiro

AU - Sato, Takako

AU - Nyunoya, Hiroshi

AU - Tsujimoto, Atsumi

AU - Takahara, Jiro

AU - Irino, Shozo

AU - Shimotohno, Kunitada

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