Isolation of a cDNA for a nucleoside diphosphate kinase capable of phosphorylating the kinase domain of the self-incompatibility factor SRK of Brassica campestris

Yasuhiko Matsushita, Tatsuya Suzuki, Ryo Kubota, Masako Mori, Hiroko Shimosato, Masao Watanabe, Toshiaki Kayano, Takeshi Nishio, Hiroshi Nyunoya

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

SRK is a plant receptor kinase involved in the self-incompatibility system of Brassica species. During a cDNA screening for the phosphoproteins from a stigma expression library, a clone encoding the nucleoside diphosphate kinase III (Bc-NDPK III) was obtained. After in vitro phosphorylation assays with recombinant proteins, Bc-NDPK III contained mostly phosphoserine. By contrast, the kinase domain of SRK contained phosphoserine and phosphothreonine, both of which were significantly increased by the addition of Bc-NDPK III in the presence of an SRK inhibitor KN-62. The result suggested the possible involvement of Bc-NDPK III in the signal transduction pathway through SRK.

Original languageEnglish
Pages (from-to)765-767
Number of pages3
JournalJournal of Experimental Botany
Volume53
Issue number369
DOIs
Publication statusPublished - 2002 Jan 1
Externally publishedYes

Keywords

  • Brassica campestris
  • Nucleoside diphosphate kinase
  • SRK
  • Self-incompatibility factor

ASJC Scopus subject areas

  • Physiology
  • Plant Science

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