Isolation of a cDNA for a nucleoside diphosphate kinase capable of phosphorylating the kinase domain of the self-incompatibility factor SRK of Brassica campestris

Yasuhiko Matsushita, Tatsuya Suzuki, Ryo Kubota, Masako Mori, Hiroko Shimosato, Masao Watanabe, Toshiaki Kayano, Takeshi Nishio, Hiroshi Nyunoya

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

SRK is a plant receptor kinase involved in the self-incompatibility system of Brassica species. During a cDNA screening for the phosphoproteins from a stigma expression library, a clone encoding the nucleoside diphosphate kinase III (Bc-NDPK III) was obtained. After in vitro phosphorylation assays with recombinant proteins, Bc-NDPK III contained mostly phosphoserine. By contrast, the kinase domain of SRK contained phosphoserine and phosphothreonine, both of which were significantly increased by the addition of Bc-NDPK III in the presence of an SRK inhibitor KN-62. The result suggested the possible involvement of Bc-NDPK III in the signal transduction pathway through SRK.

Original languageEnglish
Pages (from-to)765-767
Number of pages3
JournalJournal of Experimental Botany
Volume53
Issue number369
Publication statusPublished - 2002
Externally publishedYes

Fingerprint

Brassica rapa subsp. oleifera
Nucleoside-Diphosphate Kinase
nucleoside-diphosphate kinase
Phosphoserine
KN 62
Brassica
phosphotransferases (kinases)
Phosphotransferases
Complementary DNA
Phosphothreonine
phosphoproteins
Phosphoproteins
stigma
Recombinant Proteins
recombinant proteins
signal transduction
Signal Transduction
phosphorylation
Clone Cells
Phosphorylation

Keywords

  • Brassica campestris
  • Nucleoside diphosphate kinase
  • Self-incompatibility factor
  • SRK

ASJC Scopus subject areas

  • Physiology
  • Plant Science

Cite this

Isolation of a cDNA for a nucleoside diphosphate kinase capable of phosphorylating the kinase domain of the self-incompatibility factor SRK of Brassica campestris. / Matsushita, Yasuhiko; Suzuki, Tatsuya; Kubota, Ryo; Mori, Masako; Shimosato, Hiroko; Watanabe, Masao; Kayano, Toshiaki; Nishio, Takeshi; Nyunoya, Hiroshi.

In: Journal of Experimental Botany, Vol. 53, No. 369, 2002, p. 765-767.

Research output: Contribution to journalArticle

Matsushita, Yasuhiko ; Suzuki, Tatsuya ; Kubota, Ryo ; Mori, Masako ; Shimosato, Hiroko ; Watanabe, Masao ; Kayano, Toshiaki ; Nishio, Takeshi ; Nyunoya, Hiroshi. / Isolation of a cDNA for a nucleoside diphosphate kinase capable of phosphorylating the kinase domain of the self-incompatibility factor SRK of Brassica campestris. In: Journal of Experimental Botany. 2002 ; Vol. 53, No. 369. pp. 765-767.
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AU - Suzuki, Tatsuya

AU - Kubota, Ryo

AU - Mori, Masako

AU - Shimosato, Hiroko

AU - Watanabe, Masao

AU - Kayano, Toshiaki

AU - Nishio, Takeshi

AU - Nyunoya, Hiroshi

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