Isolation of a novel human gene from the Down syndrome critical region of chromosome 21q22.2

Akiko Nakamura, Masahira Hattori, Yoshiyuki Sakaki

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Down syndrome is the most common birth defect, and is caused by trisomy 21. We identified a novel gene in the so-called Down syndrome critical region by means of computer-aided exon prediction and subsequent cDNA cloning. The gene, designated as DCRA (Down syndrome Critical Region gene A), consists of eight exons of 3252 bp in total and encodes a large open reading frame of 297 amino acid residues. The open reading frame shows significant homology to Hβ58, a mouse gene essential for embryogenesis, PEP8, a yeast homologue of Hβ58, and an expressed sequence tag of Arabidopsis thariana, suggesting that DCRA has some important function that has been conserved during the course of evolution. DCRA is expressed in most tissues examined, including fetal and adult brain, heart, lung, liver, and kidney. The cDNA of the DCRA mouse homologue, Dcra, was also cloned. It is 2157 bp long and has an open reading frame of 297 amino acid residues, which shows 92% identity to human DCRA. Dcra is expressed in all the embryo and adult tissues examined.

Original languageEnglish
Pages (from-to)872-877
Number of pages6
JournalJournal of Biochemistry
Volume122
Issue number4
Publication statusPublished - 1997 Oct
Externally publishedYes

Fingerprint

Chromosomes
Genes
Open Reading Frames
Down Syndrome
Exons
Complementary DNA
Amino Acids
Tissue
Essential Genes
Expressed Sequence Tags
Down Syndrome Critical Region
Cloning
Arabidopsis
Embryonic Development
Organism Cloning
Liver
Yeast
Embryonic Structures
Yeasts
Brain

Keywords

  • cDNA cloning
  • Chromosome 21
  • Computer-aided exon prediction
  • Down syndrome

ASJC Scopus subject areas

  • Biochemistry

Cite this

Isolation of a novel human gene from the Down syndrome critical region of chromosome 21q22.2. / Nakamura, Akiko; Hattori, Masahira; Sakaki, Yoshiyuki.

In: Journal of Biochemistry, Vol. 122, No. 4, 10.1997, p. 872-877.

Research output: Contribution to journalArticle

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AB - Down syndrome is the most common birth defect, and is caused by trisomy 21. We identified a novel gene in the so-called Down syndrome critical region by means of computer-aided exon prediction and subsequent cDNA cloning. The gene, designated as DCRA (Down syndrome Critical Region gene A), consists of eight exons of 3252 bp in total and encodes a large open reading frame of 297 amino acid residues. The open reading frame shows significant homology to Hβ58, a mouse gene essential for embryogenesis, PEP8, a yeast homologue of Hβ58, and an expressed sequence tag of Arabidopsis thariana, suggesting that DCRA has some important function that has been conserved during the course of evolution. DCRA is expressed in most tissues examined, including fetal and adult brain, heart, lung, liver, and kidney. The cDNA of the DCRA mouse homologue, Dcra, was also cloned. It is 2157 bp long and has an open reading frame of 297 amino acid residues, which shows 92% identity to human DCRA. Dcra is expressed in all the embryo and adult tissues examined.

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