Isolation of cDNAs for DNA-binding proteins which specifically bind to a tax-responsive enhancer element in the long terminal repeat of human T-cell leukemia virus type I

Atsumi Tsujimoto, Hiroshi Nyunoya, Toshiro Morita, Takako Sato, Kunitada Shimotohno

Research output: Contribution to journalArticle

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Abstract

One of the gene products of human T-cell leukemia virus type I (HTLV-I), p40tax activates its own viral transcription in trans through tax-responsive enhancers in viral long terminal repeats. Five species of cDNA clones for proteins that bind to the tax-responsive enhancer element in HTLV-I were isolated front the Jurkat cell library. The β-galactosidase fusion protein prepared from the lysogen of a clone specifically recognized the cyclic AMP-responsive element in HTLV-I enhancer. The nucleotide sequence of a full-length cDNA clone (TAXREB67) had a coding capacity of 351 amino acids, which contained a basic motif followed by a leucine zipper structure near the carboxy terminus. Its mRNA was detected in human cell lines, including HTLV-I-infected or noninfected hematopoietic cell lines. The mRNA level in Jurkat cells was decreased temporarily by increasing cyclic AMP concentration but increased by increasing Ca2+ concentration. Polyclonal antibodies against the fusion protein specifically recognized a 52-kDa protein in Jurkat cells. Analyses of the function of this protein and its interactions with other cellular factors will be useful to help understand the regulatory mechanism through tax-responsive enhancers in HTLV-I.

Original languageEnglish
Pages (from-to)1420-1426
Number of pages7
JournalJournal of Virology
Volume65
Issue number3
Publication statusPublished - 1991
Externally publishedYes

Fingerprint

Primate T-lymphotropic virus 1
enhancer elements
Human T-lymphotropic virus 1
terminal repeat sequences
DNA-binding proteins
Terminal Repeat Sequences
DNA-Binding Proteins
taxes
Complementary DNA
Jurkat Cells
Clone Cells
cyclic AMP
clones
Proteins
proteins
Cyclic AMP
Galactosidases
galactosidases
Cell Line
Leucine Zippers

ASJC Scopus subject areas

  • Immunology

Cite this

Isolation of cDNAs for DNA-binding proteins which specifically bind to a tax-responsive enhancer element in the long terminal repeat of human T-cell leukemia virus type I. / Tsujimoto, Atsumi; Nyunoya, Hiroshi; Morita, Toshiro; Sato, Takako; Shimotohno, Kunitada.

In: Journal of Virology, Vol. 65, No. 3, 1991, p. 1420-1426.

Research output: Contribution to journalArticle

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