Isolation of Magnetospirillum magneticum AMB-1 mutants defective in bacterial magnetic particle synthesis by transposon mutagenesis

Aris Tri Wahyudi, Haruko Takeyama, Tadashi Matsunaga

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Nonmagnetic mutants of Magnetospirillum magneticum AMB-1 were recovered following mini-Tn5 transposon mutagenesis. Transconjugants with kanamycin resistance were obtained at a frequency of 2.7 × 10-7 per recipient. Of 3327 transconjugants, 62 were defective for bacterial magnetic particle (BMP) synthesis. The frequency of independent transposition events for nonmagnetic mutants was about 1.4% in transconjugants. Further analysis of DNA sequences flanking transposon by inverted polymerase chain reaction allowed isolation of at least 10 genes or DNA sequences involved in BMP synthesis in M. magneticum AMB-1.

Original languageEnglish
Pages (from-to)147-154
Number of pages8
JournalApplied Biochemistry and Biotechnology - Part A Enzyme Engineering and Biotechnology
Volume91-93
DOIs
Publication statusPublished - 2001 May 23
Externally publishedYes

Keywords

  • Bacterial magnetic particles
  • Inverse polymerase chain reaction
  • Magnetospirillum magneticum AMB-1
  • Mini-Tn5
  • Sequence analysis
  • Transposon mutagenesis

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology

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