Isolation of Magnetospirillum magneticum AMB-1 mutants defective in bacterial magnetic particle synthesis by transposon mutagenesis

Aris Tri Wahyudi, Haruko Takeyama, Tadashi Matsunaga

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Nonmagnetic mutants of Magnetospirillum magneticum AMB-1 were recovered following mini-Tn5 transposon mutagenesis. Transconjugants with kanamycin resistance were obtained at a frequency of 2.7 × 10-7 per recipient. Of 3327 transconjugants, 62 were defective for bacterial magnetic particle (BMP) synthesis. The frequency of independent transposition events for nonmagnetic mutants was about 1.4% in transconjugants. Further analysis of DNA sequences flanking transposon by inverted polymerase chain reaction allowed isolation of at least 10 genes or DNA sequences involved in BMP synthesis in M. magneticum AMB-1.

Original languageEnglish
Pages (from-to)147-154
Number of pages8
JournalApplied Biochemistry and Biotechnology - Part A Enzyme Engineering and Biotechnology
Volume91-93
DOIs
Publication statusPublished - 2001
Externally publishedYes

Fingerprint

Magnetospirillum
Kanamycin Resistance
Mutagenesis
DNA sequences
DNA Sequence Analysis
Polymerase Chain Reaction
Kanamycin
Polymerase chain reaction
Genes

Keywords

  • Bacterial magnetic particles
  • Inverse polymerase chain reaction
  • Magnetospirillum magneticum AMB-1
  • Mini-Tn5
  • Sequence analysis
  • Transposon mutagenesis

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Biotechnology
  • Bioengineering

Cite this

@article{93868c2b2d3748f09968fb7421a25d36,
title = "Isolation of Magnetospirillum magneticum AMB-1 mutants defective in bacterial magnetic particle synthesis by transposon mutagenesis",
abstract = "Nonmagnetic mutants of Magnetospirillum magneticum AMB-1 were recovered following mini-Tn5 transposon mutagenesis. Transconjugants with kanamycin resistance were obtained at a frequency of 2.7 × 10-7 per recipient. Of 3327 transconjugants, 62 were defective for bacterial magnetic particle (BMP) synthesis. The frequency of independent transposition events for nonmagnetic mutants was about 1.4{\%} in transconjugants. Further analysis of DNA sequences flanking transposon by inverted polymerase chain reaction allowed isolation of at least 10 genes or DNA sequences involved in BMP synthesis in M. magneticum AMB-1.",
keywords = "Bacterial magnetic particles, Inverse polymerase chain reaction, Magnetospirillum magneticum AMB-1, Mini-Tn5, Sequence analysis, Transposon mutagenesis",
author = "Wahyudi, {Aris Tri} and Haruko Takeyama and Tadashi Matsunaga",
year = "2001",
doi = "10.1385/ABAB:91-93:1-9:147",
language = "English",
volume = "91-93",
pages = "147--154",
journal = "Applied Biochemistry and Biotechnology",
issn = "0273-2289",
publisher = "Humana Press",

}

TY - JOUR

T1 - Isolation of Magnetospirillum magneticum AMB-1 mutants defective in bacterial magnetic particle synthesis by transposon mutagenesis

AU - Wahyudi, Aris Tri

AU - Takeyama, Haruko

AU - Matsunaga, Tadashi

PY - 2001

Y1 - 2001

N2 - Nonmagnetic mutants of Magnetospirillum magneticum AMB-1 were recovered following mini-Tn5 transposon mutagenesis. Transconjugants with kanamycin resistance were obtained at a frequency of 2.7 × 10-7 per recipient. Of 3327 transconjugants, 62 were defective for bacterial magnetic particle (BMP) synthesis. The frequency of independent transposition events for nonmagnetic mutants was about 1.4% in transconjugants. Further analysis of DNA sequences flanking transposon by inverted polymerase chain reaction allowed isolation of at least 10 genes or DNA sequences involved in BMP synthesis in M. magneticum AMB-1.

AB - Nonmagnetic mutants of Magnetospirillum magneticum AMB-1 were recovered following mini-Tn5 transposon mutagenesis. Transconjugants with kanamycin resistance were obtained at a frequency of 2.7 × 10-7 per recipient. Of 3327 transconjugants, 62 were defective for bacterial magnetic particle (BMP) synthesis. The frequency of independent transposition events for nonmagnetic mutants was about 1.4% in transconjugants. Further analysis of DNA sequences flanking transposon by inverted polymerase chain reaction allowed isolation of at least 10 genes or DNA sequences involved in BMP synthesis in M. magneticum AMB-1.

KW - Bacterial magnetic particles

KW - Inverse polymerase chain reaction

KW - Magnetospirillum magneticum AMB-1

KW - Mini-Tn5

KW - Sequence analysis

KW - Transposon mutagenesis

UR - http://www.scopus.com/inward/record.url?scp=0035026023&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0035026023&partnerID=8YFLogxK

U2 - 10.1385/ABAB:91-93:1-9:147

DO - 10.1385/ABAB:91-93:1-9:147

M3 - Article

C2 - 11963843

AN - SCOPUS:0035026023

VL - 91-93

SP - 147

EP - 154

JO - Applied Biochemistry and Biotechnology

JF - Applied Biochemistry and Biotechnology

SN - 0273-2289

ER -