Isolation of Nitrospira belonging to sublineage II from a wastewater treatment plant

Norisuke Ushiki, Hirotsugu Fujitani, Yoshiteru Aoi, Satoshi Tsuneda

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

Nitrite oxidation is a key step in nitrogen removal in biological wastewater treatment plants. Recently, two phylogenetically different Nitrospira (sublineages I and II) have been recognized as the numerically dominant nitriteoxidizing bacteria in wastewater treatment plants. However, Nitrospira sublineage II inhabiting activated sludge was not isolated and its detailed properties were unclear. In this study, we developed a new method for the isolation of Nitrospira forming micro-colonies using a cell sorter. We obtained a novel pure strain "Nitrospira japonica" from the activated sludge. Subsequently, phylogenetic and physiological analyses revealed that Nitrospira japonica belongs to sublineage II and grew in medium containing formate. This method has the potential to isolate other uncultured microorganisms forming micro-colonies.

Original languageEnglish
Pages (from-to)346-353
Number of pages8
JournalMicrobes and Environments
Volume28
Issue number3
DOIs
Publication statusPublished - 2013

Fingerprint

activated sludge
wastewater treatment
formates
nitrites
nitrite
microorganism
oxidation
phylogenetics
microorganisms
bacterium
nitrogen
phylogeny
bacteria
methodology
cells
wastewater treatment plant
method
biological wastewater treatment
removal

Keywords

  • Cell sorter
  • Formate
  • Micro-colony
  • Nitrification
  • Uncultured bacteria

ASJC Scopus subject areas

  • Plant Science
  • Soil Science
  • Ecology, Evolution, Behavior and Systematics

Cite this

Isolation of Nitrospira belonging to sublineage II from a wastewater treatment plant. / Ushiki, Norisuke; Fujitani, Hirotsugu; Aoi, Yoshiteru; Tsuneda, Satoshi.

In: Microbes and Environments, Vol. 28, No. 3, 2013, p. 346-353.

Research output: Contribution to journalArticle

Ushiki, Norisuke ; Fujitani, Hirotsugu ; Aoi, Yoshiteru ; Tsuneda, Satoshi. / Isolation of Nitrospira belonging to sublineage II from a wastewater treatment plant. In: Microbes and Environments. 2013 ; Vol. 28, No. 3. pp. 346-353.
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