Isolation of replicational cue elements from a library of bent DNAs of Aspergillus oryzae

Takahiro Kusakabe, Yasushi Sugimoto, Yoshiko Hirota, Shigenobu Toné, Yutaka Kawaguchi, Katsumi Koga, Takashi Ohyama

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Two fragments that could function as replicational cue elements were isolated from a genomic DNA digest of Aspergillus oryzae on the basis of abnormal behavior in polyacrylamide gel electrophoresis. The vector used in this study contained a scaffold-associated region of the Drosophila melanogaster ftz gene to provide nuclear retention. Neither fragment contained a yeast ARS consensus sequence or an eukaryotic topoisomerase II binding sequence. One of the fragments showed sequence homology with the mitochondrial replication origin of Candida utilis and a portion of mitochondrial DNA of Aspergillus nidulans. This plasmid carrying the cue fragment could also replicate in HeLa and NIH3T3 cells.

Original languageEnglish
Pages (from-to)13-19
Number of pages7
JournalMolecular Biology Reports
Volume27
Issue number1
Publication statusPublished - 2000
Externally publishedYes

Fingerprint

Aspergillus oryzae
Aspergillus
Gene Library
Cues
Aspergillus nidulans
Type II DNA Topoisomerase
Replication Origin
Candida
DNA
Consensus Sequence
Sequence Homology
Electrophoresis
Drosophila melanogaster
Mitochondrial DNA
HeLa Cells
Scaffolds
Yeast
Polyacrylamide Gel Electrophoresis
Plasmids
Genes

Keywords

  • Autonomously replicating sequence
  • Fungal genome
  • Scaffold-associated region
  • Unusual DNA structure

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Genetics

Cite this

Kusakabe, T., Sugimoto, Y., Hirota, Y., Toné, S., Kawaguchi, Y., Koga, K., & Ohyama, T. (2000). Isolation of replicational cue elements from a library of bent DNAs of Aspergillus oryzae. Molecular Biology Reports, 27(1), 13-19.

Isolation of replicational cue elements from a library of bent DNAs of Aspergillus oryzae. / Kusakabe, Takahiro; Sugimoto, Yasushi; Hirota, Yoshiko; Toné, Shigenobu; Kawaguchi, Yutaka; Koga, Katsumi; Ohyama, Takashi.

In: Molecular Biology Reports, Vol. 27, No. 1, 2000, p. 13-19.

Research output: Contribution to journalArticle

Kusakabe, T, Sugimoto, Y, Hirota, Y, Toné, S, Kawaguchi, Y, Koga, K & Ohyama, T 2000, 'Isolation of replicational cue elements from a library of bent DNAs of Aspergillus oryzae', Molecular Biology Reports, vol. 27, no. 1, pp. 13-19.
Kusakabe T, Sugimoto Y, Hirota Y, Toné S, Kawaguchi Y, Koga K et al. Isolation of replicational cue elements from a library of bent DNAs of Aspergillus oryzae. Molecular Biology Reports. 2000;27(1):13-19.
Kusakabe, Takahiro ; Sugimoto, Yasushi ; Hirota, Yoshiko ; Toné, Shigenobu ; Kawaguchi, Yutaka ; Koga, Katsumi ; Ohyama, Takashi. / Isolation of replicational cue elements from a library of bent DNAs of Aspergillus oryzae. In: Molecular Biology Reports. 2000 ; Vol. 27, No. 1. pp. 13-19.
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