Isolation of replicational cue elements from a library of bent DNAs of Aspergillus oryzae

Takahiro Kusakabe, Yasushi Sugimoto, Yoshiko Hirota, Shigenobu Toné, Yutaka Kawaguchi, Katsumi Koga, Takashi Ohyama

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Two fragments that could function as replicational cue elements were isolated from a genomic DNA digest of Aspergillus oryzae on the basis of abnormal behavior in polyacrylamide gel electrophoresis. The vector used in this study contained a scaffold-associated region of the Drosophila melanogaster ftz gene to provide nuclear retention. Neither fragment contained a yeast ARS consensus sequence or an eukaryotic topoisomerase II binding sequence. One of the fragments showed sequence homology with the mitochondrial replication origin of Candida utilis and a portion of mitochondrial DNA of Aspergillus nidulans. This plasmid carrying the cue fragment could also replicate in HeLa and NIH3T3 cells.

Original languageEnglish
Pages (from-to)13-19
Number of pages7
JournalMolecular Biology Reports
Volume27
Issue number1
DOIs
Publication statusPublished - 2000 Jan 1
Externally publishedYes

Keywords

  • Autonomously replicating sequence
  • Fungal genome
  • Scaffold-associated region
  • Unusual DNA structure

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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