Isolation of sublineage I Nitrospira by a novel cultivation strategy

Hirotsugu Fujitani, Norisuke Ushiki, Satoshi Tsuneda, Yoshiteru Aoi

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Nitrification is an important process in the biogeochemical nitrogen cycle and is widely exploited in biological wastewater treatment. Recently, Nitrospira has been recognized as the numerically dominant nitrite-oxidizing bacterial genus and is primarily responsible for the second step of aerobic nitrification. Nevertheless, the physiological properties of Nitrospira remain poorly understood because the organisms are difficult to isolate and culture. Here, we report a novel cultivation strategy for obtaining members of the Nitrospira sublineage I in pure culture. The method combines: (i) selective enrichment of Nitrospira using a continuous feeding reactor and (ii) purification followed by sub-cultivation via a cell sorting system by focusing on the unique characteristics of Nitrospira forming spherical micro-colonies. This strategy is potentially applicable to other uncultured or unisolated Nitrospira and could accelerate the physiological and biochemical understandings of this important group of organisms.

Original languageEnglish
Pages (from-to)3030-3040
Number of pages11
JournalEnvironmental Microbiology
Volume16
Issue number10
DOIs
Publication statusPublished - 2014 Oct 1

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Nitrification
nitrification
Nitrogen Cycle
nitrogen cycle
organisms
Nitrites
Waste Water
wastewater treatment
sorting
nitrites
nitrite
purification
cells
organism
methodology
reactor
biological wastewater treatment
method

ASJC Scopus subject areas

  • Microbiology
  • Ecology, Evolution, Behavior and Systematics

Cite this

Isolation of sublineage I Nitrospira by a novel cultivation strategy. / Fujitani, Hirotsugu; Ushiki, Norisuke; Tsuneda, Satoshi; Aoi, Yoshiteru.

In: Environmental Microbiology, Vol. 16, No. 10, 01.10.2014, p. 3030-3040.

Research output: Contribution to journalArticle

Fujitani, Hirotsugu ; Ushiki, Norisuke ; Tsuneda, Satoshi ; Aoi, Yoshiteru. / Isolation of sublineage I Nitrospira by a novel cultivation strategy. In: Environmental Microbiology. 2014 ; Vol. 16, No. 10. pp. 3030-3040.
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