Isolation of two members of the rat MAP kinase kinase gene family

Masayuki Otsu*, Yasuhiko Terada, Hiroto Okayama

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

19 Citations (Scopus)


Mitogen-activated protein (MAP) kinase kinase (MAPKK) is a recently characterized activator of MAP kinase (MAPK), and is considered to be regulated by a protooncogene product c-Raf-1. It is, however, unclear whether the signals originating from c-Raf-1 utilize this phosphorylation cascade to lead to oncogenesis. To clarify this point, we isolated rat MAPKK cDNAs, and identified two distinct cDNAs encoding MAPKK and a highly related kinase, both with molecular weights of 4̃5 kDa (MEK1 and MEK2). Genomic Southern blot analyses suggested that MAPKK. may form a large gene family.

Original languageEnglish
Pages (from-to)246-250
Number of pages5
JournalFEBS Letters
Issue number3
Publication statusPublished - 1993 Apr 12
Externally publishedYes


  • MAP kinase kinase
  • Molecular cloning
  • Signal transduction

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology


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