Isolation of two members of the rat MAP kinase kinase gene family

Masayuki Otsu, Yasuhiko Terada, Hiroto Okayama

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Mitogen-activated protein (MAP) kinase kinase (MAPKK) is a recently characterized activator of MAP kinase (MAPK), and is considered to be regulated by a protooncogene product c-Raf-1. It is, however, unclear whether the signals originating from c-Raf-1 utilize this phosphorylation cascade to lead to oncogenesis. To clarify this point, we isolated rat MAPKK cDNAs, and identified two distinct cDNAs encoding MAPKK and a highly related kinase, both with molecular weights of 4̃5 kDa (MEK1 and MEK2). Genomic Southern blot analyses suggested that MAPKK. may form a large gene family.

Original languageEnglish
Pages (from-to)246-250
Number of pages5
JournalFEBS Letters
Volume320
Issue number3
DOIs
Publication statusPublished - 1993 Apr 12
Externally publishedYes

Fingerprint

Mitogen-Activated Protein Kinase Kinases
Rats
Genes
Complementary DNA
Phosphorylation
Southern Blotting
Mitogen-Activated Protein Kinases
Carcinogenesis
Phosphotransferases
Molecular Weight
Molecular weight

Keywords

  • MAP kinase kinase
  • Molecular cloning
  • Signal transduction

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Isolation of two members of the rat MAP kinase kinase gene family. / Otsu, Masayuki; Terada, Yasuhiko; Okayama, Hiroto.

In: FEBS Letters, Vol. 320, No. 3, 12.04.1993, p. 246-250.

Research output: Contribution to journalArticle

Otsu, Masayuki ; Terada, Yasuhiko ; Okayama, Hiroto. / Isolation of two members of the rat MAP kinase kinase gene family. In: FEBS Letters. 1993 ; Vol. 320, No. 3. pp. 246-250.
@article{3cd5b34f019f4231ba925a5fac33d61f,
title = "Isolation of two members of the rat MAP kinase kinase gene family",
abstract = "Mitogen-activated protein (MAP) kinase kinase (MAPKK) is a recently characterized activator of MAP kinase (MAPK), and is considered to be regulated by a protooncogene product c-Raf-1. It is, however, unclear whether the signals originating from c-Raf-1 utilize this phosphorylation cascade to lead to oncogenesis. To clarify this point, we isolated rat MAPKK cDNAs, and identified two distinct cDNAs encoding MAPKK and a highly related kinase, both with molecular weights of 4̃5 kDa (MEK1 and MEK2). Genomic Southern blot analyses suggested that MAPKK. may form a large gene family.",
keywords = "MAP kinase kinase, Molecular cloning, Signal transduction",
author = "Masayuki Otsu and Yasuhiko Terada and Hiroto Okayama",
year = "1993",
month = "4",
day = "12",
doi = "10.1016/0014-5793(93)80596-M",
language = "English",
volume = "320",
pages = "246--250",
journal = "FEBS Letters",
issn = "0014-5793",
publisher = "Elsevier",
number = "3",

}

TY - JOUR

T1 - Isolation of two members of the rat MAP kinase kinase gene family

AU - Otsu, Masayuki

AU - Terada, Yasuhiko

AU - Okayama, Hiroto

PY - 1993/4/12

Y1 - 1993/4/12

N2 - Mitogen-activated protein (MAP) kinase kinase (MAPKK) is a recently characterized activator of MAP kinase (MAPK), and is considered to be regulated by a protooncogene product c-Raf-1. It is, however, unclear whether the signals originating from c-Raf-1 utilize this phosphorylation cascade to lead to oncogenesis. To clarify this point, we isolated rat MAPKK cDNAs, and identified two distinct cDNAs encoding MAPKK and a highly related kinase, both with molecular weights of 4̃5 kDa (MEK1 and MEK2). Genomic Southern blot analyses suggested that MAPKK. may form a large gene family.

AB - Mitogen-activated protein (MAP) kinase kinase (MAPKK) is a recently characterized activator of MAP kinase (MAPK), and is considered to be regulated by a protooncogene product c-Raf-1. It is, however, unclear whether the signals originating from c-Raf-1 utilize this phosphorylation cascade to lead to oncogenesis. To clarify this point, we isolated rat MAPKK cDNAs, and identified two distinct cDNAs encoding MAPKK and a highly related kinase, both with molecular weights of 4̃5 kDa (MEK1 and MEK2). Genomic Southern blot analyses suggested that MAPKK. may form a large gene family.

KW - MAP kinase kinase

KW - Molecular cloning

KW - Signal transduction

UR - http://www.scopus.com/inward/record.url?scp=0027408258&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027408258&partnerID=8YFLogxK

U2 - 10.1016/0014-5793(93)80596-M

DO - 10.1016/0014-5793(93)80596-M

M3 - Article

C2 - 8462694

AN - SCOPUS:0027408258

VL - 320

SP - 246

EP - 250

JO - FEBS Letters

JF - FEBS Letters

SN - 0014-5793

IS - 3

ER -