TY - JOUR
T1 - Isozymes of superoxide dismutase in chlamydomonas and purification of one of the major isozymes containing fe
AU - Sakurai, Hidehiro
AU - Kusumoto, Noriaki
AU - Kitayama, Kaoru
AU - Togasaki, Robert K.
N1 - Funding Information:
This work was aided in part by Grant-in-Aid for Scientific Research on Priority Areas from the Ministry of Education, Science and Culture, Japan (04273216) to H.S. and a grant from Nippon Zeon Co. to R.K.T. We would like to thank Ms. Susan J. Carlson for critically reading the manuscript and to Mr. H. Imai for amino acid sequencing.
PY - 1993/10
Y1 - 1993/10
N2 - Electrophoretic analysis of Chlamydomonas reinhardtii extract revealed at least 4 distinct superoxide dismutase (SOD) activity bands as well as several additional minor bands. Among them, one was deduced to be Fe-type and the other three Mn-type based on their susceptibility to KCN and H2O2. The Fe-SOD, which occupied about 40% of the total soluble activity, was purified to homogeneity using ammonium sulfate fractionation followed by DEAE-cellulose, hydroxyapatite, and Superdex 75 gel-permeation chromatography. The 40-kDa native enzyme was composed of two identical 20-kDa subunits with a low shoulder of absorption at ̃350 nm. The NH2-terminal amino acid sequence determined up to residue 29 showed a high homology to those of Fe-SOD from Arabidopsis thaliana, Glycine max, and Nicotiana plumbaginifolia.
AB - Electrophoretic analysis of Chlamydomonas reinhardtii extract revealed at least 4 distinct superoxide dismutase (SOD) activity bands as well as several additional minor bands. Among them, one was deduced to be Fe-type and the other three Mn-type based on their susceptibility to KCN and H2O2. The Fe-SOD, which occupied about 40% of the total soluble activity, was purified to homogeneity using ammonium sulfate fractionation followed by DEAE-cellulose, hydroxyapatite, and Superdex 75 gel-permeation chromatography. The 40-kDa native enzyme was composed of two identical 20-kDa subunits with a low shoulder of absorption at ̃350 nm. The NH2-terminal amino acid sequence determined up to residue 29 showed a high homology to those of Fe-SOD from Arabidopsis thaliana, Glycine max, and Nicotiana plumbaginifolia.
KW - Amino acid sequence
KW - Chlamydomonas reinhardtii
KW - Fe-SOD
KW - Superoxide dismutase
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M3 - Article
AN - SCOPUS:0027136468
VL - 34
SP - 1133
EP - 1137
JO - Plant and Cell Physiology
JF - Plant and Cell Physiology
SN - 0032-0781
IS - 7
ER -