Abstract
Electrophoretic analysis of Chlamydomonas reinhardtii extract revealed at least 4 distinct superoxide dismutase (SOD) activity bands as well as several additional minor bands. Among them, one was deduced to be Fe-type and the other three Mn-type based on their susceptibility to KCN and H2O2. The Fe-SOD, which occupied about 40% of the total soluble activity, was purified to homogeneity using ammonium sulfate fractionation followed by DEAE-cellulose, hydroxyapatite, and Superdex 75 gel-permeation chromatography. The 40-kDa native enzyme was composed of two identical 20-kDa subunits with a low shoulder of absorption at ̃350 nm. The NH2-terminal amino acid sequence determined up to residue 29 showed a high homology to those of Fe-SOD from Arabidopsis thaliana, Glycine max, and Nicotiana plumbaginifolia.
Original language | English |
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Pages (from-to) | 1133-1137 |
Number of pages | 5 |
Journal | Plant and Cell Physiology |
Volume | 34 |
Issue number | 7 |
Publication status | Published - 1993 Oct 1 |
Keywords
- Amino acid sequence
- Chlamydomonas reinhardtii
- Fe-SOD
- Superoxide dismutase
ASJC Scopus subject areas
- Physiology
- Plant Science
- Cell Biology