Abstract
Electrophoretic analysis of Chlamydomonas reinhardtii extract revealed at least 4 distinct superoxide dismutase (SOD) activity bands as well as several additional minor bands. Among them, one was deduced to be Fe-type and the other three Mn-type based on their susceptibility to KCN and H2O2. The Fe-SOD, which occupied about 40% of the total soluble activity, was purified to homogeneity using ammonium sulfate fractionation followed by DEAE-cellulose, hydroxyapatite, and Superdex 75 gel-permeation chromatography. The 40-kDa native enzyme was composed of two identical 20-kDa subunits with a low shoulder of absorption at ̃350 nm. The NH2-terminal amino acid sequence determined up to residue 29 showed a high homology to those of Fe-SOD from Arabidopsis thaliana, Glycine max, and Nicotiana plumbaginifolia.
| Original language | English |
|---|---|
| Pages (from-to) | 1133-1137 |
| Number of pages | 5 |
| Journal | Plant and Cell Physiology |
| Volume | 34 |
| Issue number | 7 |
| Publication status | Published - 1993 Oct |
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Keywords
- Amino acid sequence
- Chlamydomonas reinhardtii
- Fe-SOD
- Superoxide dismutase
ASJC Scopus subject areas
- Statistics, Probability and Uncertainty
- Applied Mathematics
- Ecology
- Cell Biology
- Physiology
- Plant Science
Cite this
Isozymes of superoxide dismutase in chlamydomonas and purification of one of the major isozymes containing fe. / Sakurai, Hidehiro; Kusumoto, Noriaki; Kitayama, Kaoru; Togasaki, Robert K.
In: Plant and Cell Physiology, Vol. 34, No. 7, 10.1993, p. 1133-1137.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Isozymes of superoxide dismutase in chlamydomonas and purification of one of the major isozymes containing fe
AU - Sakurai, Hidehiro
AU - Kusumoto, Noriaki
AU - Kitayama, Kaoru
AU - Togasaki, Robert K.
PY - 1993/10
Y1 - 1993/10
N2 - Electrophoretic analysis of Chlamydomonas reinhardtii extract revealed at least 4 distinct superoxide dismutase (SOD) activity bands as well as several additional minor bands. Among them, one was deduced to be Fe-type and the other three Mn-type based on their susceptibility to KCN and H2O2. The Fe-SOD, which occupied about 40% of the total soluble activity, was purified to homogeneity using ammonium sulfate fractionation followed by DEAE-cellulose, hydroxyapatite, and Superdex 75 gel-permeation chromatography. The 40-kDa native enzyme was composed of two identical 20-kDa subunits with a low shoulder of absorption at ̃350 nm. The NH2-terminal amino acid sequence determined up to residue 29 showed a high homology to those of Fe-SOD from Arabidopsis thaliana, Glycine max, and Nicotiana plumbaginifolia.
AB - Electrophoretic analysis of Chlamydomonas reinhardtii extract revealed at least 4 distinct superoxide dismutase (SOD) activity bands as well as several additional minor bands. Among them, one was deduced to be Fe-type and the other three Mn-type based on their susceptibility to KCN and H2O2. The Fe-SOD, which occupied about 40% of the total soluble activity, was purified to homogeneity using ammonium sulfate fractionation followed by DEAE-cellulose, hydroxyapatite, and Superdex 75 gel-permeation chromatography. The 40-kDa native enzyme was composed of two identical 20-kDa subunits with a low shoulder of absorption at ̃350 nm. The NH2-terminal amino acid sequence determined up to residue 29 showed a high homology to those of Fe-SOD from Arabidopsis thaliana, Glycine max, and Nicotiana plumbaginifolia.
KW - Amino acid sequence
KW - Chlamydomonas reinhardtii
KW - Fe-SOD
KW - Superoxide dismutase
UR - http://www.scopus.com/inward/record.url?scp=0027136468&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0027136468&partnerID=8YFLogxK
M3 - Article
AN - SCOPUS:0027136468
VL - 34
SP - 1133
EP - 1137
JO - Plant and Cell Physiology
JF - Plant and Cell Physiology
SN - 0032-0781
IS - 7
ER -