Key residues on microtubule responsible for activation of kinesin ATPase

Seiichi Uchimura, Yusuke Oguchi, You Hachikubo, Shin'Ichi Ishiwata, Etsuko Muto

    Research output: Contribution to journalArticle

    33 Citations (Scopus)

    Abstract

    Microtubule (MT) binding accelerates the rate of ATP hydrolysis in kinesin. To understand the underlying mechanism, using charged-to-alanine mutational analysis, we identified two independent sites in tubulin, which are critical for kinesin motility, namely, a cluster of negatively charged residues spanning the helix 11-12 (H11-12) loop and H12 of α-tubulin, and the negatively charged residues in H12 of Β-tubulin. Mutation in the α-tubulin- binding site results in a deceleration of ATP hydrolysis (k cat), whereas mutation in the Β-tubulin-binding site lowers the affinity for MTs (K 0.5 MT). The residue E415 in α-tubulin seems to be important for coupling MT binding and ATPase activation, because the mutation at this site results in a drastic reduction in the overall rate of ATP hydrolysis, largely due to a deceleration in the reaction of ADP release. Our results suggest that kinesin binding at a region containing α-E415 could transmit a signal to the kinesin nucleotide pocket, triggering its conformational change and leading to the release of ADP.

    Original languageEnglish
    Pages (from-to)1167-1175
    Number of pages9
    JournalEMBO Journal
    Volume29
    Issue number7
    DOIs
    Publication statusPublished - 2010 Apr

    Fingerprint

    Kinesin
    Tubulin
    Microtubules
    Adenosine Triphosphatases
    Chemical activation
    Hydrolysis
    Deceleration
    Adenosine Triphosphate
    Adenosine Diphosphate
    Mutation
    Binding Sites
    Alanine
    Cats
    Nucleotides

    Keywords

    • ATPase kinetics
    • Kinesin
    • Microtubule
    • Motility
    • Mutant analysis

    ASJC Scopus subject areas

    • Molecular Biology
    • Biochemistry, Genetics and Molecular Biology(all)
    • Immunology and Microbiology(all)
    • Neuroscience(all)

    Cite this

    Uchimura, S., Oguchi, Y., Hachikubo, Y., Ishiwata, SI., & Muto, E. (2010). Key residues on microtubule responsible for activation of kinesin ATPase. EMBO Journal, 29(7), 1167-1175. https://doi.org/10.1038/emboj.2010.25

    Key residues on microtubule responsible for activation of kinesin ATPase. / Uchimura, Seiichi; Oguchi, Yusuke; Hachikubo, You; Ishiwata, Shin'Ichi; Muto, Etsuko.

    In: EMBO Journal, Vol. 29, No. 7, 04.2010, p. 1167-1175.

    Research output: Contribution to journalArticle

    Uchimura, S, Oguchi, Y, Hachikubo, Y, Ishiwata, SI & Muto, E 2010, 'Key residues on microtubule responsible for activation of kinesin ATPase', EMBO Journal, vol. 29, no. 7, pp. 1167-1175. https://doi.org/10.1038/emboj.2010.25
    Uchimura S, Oguchi Y, Hachikubo Y, Ishiwata SI, Muto E. Key residues on microtubule responsible for activation of kinesin ATPase. EMBO Journal. 2010 Apr;29(7):1167-1175. https://doi.org/10.1038/emboj.2010.25
    Uchimura, Seiichi ; Oguchi, Yusuke ; Hachikubo, You ; Ishiwata, Shin'Ichi ; Muto, Etsuko. / Key residues on microtubule responsible for activation of kinesin ATPase. In: EMBO Journal. 2010 ; Vol. 29, No. 7. pp. 1167-1175.
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