Kinesin dependent, rapid, bi-directional transport of ER sub-compartment in dendrites of hippocampal neurons

Hiroko Bannai, Takafumi Inoue, Tomohiro Nakayama, Mitsuharu Hattori, Katsuhiko Mikoshiba

Research output: Contribution to journalArticle

78 Citations (Scopus)

Abstract

Although spatially restricted Ca2+ release from the endoplasmic reticulum (ER) through intracellular Ca2+ channels plays important roles in various neuronal activities, the accurate distribution and dynamics of ER in the dendrite of living neurons still remain unknown. To elucidate these, we expressed fluorescent protein-tagged ER proteins in cultured mouse hippocampal neurons, and monitored their movements using time-lapse microscopy. We report here that a sub-compartment of ER forms in relatively large vesicles that are capable, similarly to the reticular ER, of taking up and releasing Ca2+. The vesicular sub-compartment of ER moved rapidly along the dendrites in both anterograde and retrograde directions at a velocity of 0.2-0.3 μm/second. Depletion of microtubules, overexpression of dominant-negative kinesin and kinesin depletion by antisense DNA reduced the number and velocity of the moving vesicles, suggesting that kinesin may drive the transport of the vesicular sub-compartment of ER along microtubules in the dendrite. Rapid transport of the Ca2+-releasable sub-compartment of ER might contribute to rapid supply of fresh ER proteins to the distal part of the dendrite, or to the spatial regulation of intracellular Ca2+ signaling.

Original languageEnglish
Pages (from-to)163-175
Number of pages13
JournalJournal of Cell Science
Volume117
Issue number2
DOIs
Publication statusPublished - 2004 Jan 15
Externally publishedYes

Fingerprint

Kinesin
Dendrites
Endoplasmic Reticulum
Neurons
Microtubules
Antisense DNA
Proteins
Microscopy

Keywords

  • Dendrite
  • Endoplasmic reticulum
  • Ins(1,4,5)
  • Kinesin
  • Microtubule
  • Neuron
  • PR

ASJC Scopus subject areas

  • Cell Biology

Cite this

Kinesin dependent, rapid, bi-directional transport of ER sub-compartment in dendrites of hippocampal neurons. / Bannai, Hiroko; Inoue, Takafumi; Nakayama, Tomohiro; Hattori, Mitsuharu; Mikoshiba, Katsuhiko.

In: Journal of Cell Science, Vol. 117, No. 2, 15.01.2004, p. 163-175.

Research output: Contribution to journalArticle

Bannai, Hiroko ; Inoue, Takafumi ; Nakayama, Tomohiro ; Hattori, Mitsuharu ; Mikoshiba, Katsuhiko. / Kinesin dependent, rapid, bi-directional transport of ER sub-compartment in dendrites of hippocampal neurons. In: Journal of Cell Science. 2004 ; Vol. 117, No. 2. pp. 163-175.
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