TY - JOUR
T1 - Kinetic analysis of ribosome binding process onto mRNA using a quartz-crystal microbalance.
AU - Takahashi, Shuntaro
AU - Akita, Ryoko
AU - Furusawa, Hiroyuki
AU - Shimizu, Yoshihiro
AU - Ueda, Takuya
AU - Okahata, Yoshio
N1 - Copyright:
This record is sourced from MEDLINE/PubMed, a database of the U.S. National Library of Medicine
PY - 2006
Y1 - 2006
N2 - Translation initiation is the most dynamic and important step along a series of protein synthesis processes. In bacteria, it is generally accepted that the 70S ribosome initially dissociates into the 30S and 50S subunit, and then, the 30S ribosomal subunit binds to the Shine-Dargalno (SD) sequence of mRNA. We analyzed binding kinetics of 70S, 50S and 30S ribosomes to the SD sequence by using a mRNA-immobilized 27 MHz quartz-crystal microbalance (QCM). The 70S ribosome was found to bind strongly to the SD sequence as a ratio of 1:1 without dissociation to each subunit from the lateral side, as well as the 30S subunit. The binding constant for 70S increased in the presence of the initiator tRNA, which suggests that the SD and initiator codon of AUG could be also recognized precisely with 70S.
AB - Translation initiation is the most dynamic and important step along a series of protein synthesis processes. In bacteria, it is generally accepted that the 70S ribosome initially dissociates into the 30S and 50S subunit, and then, the 30S ribosomal subunit binds to the Shine-Dargalno (SD) sequence of mRNA. We analyzed binding kinetics of 70S, 50S and 30S ribosomes to the SD sequence by using a mRNA-immobilized 27 MHz quartz-crystal microbalance (QCM). The 70S ribosome was found to bind strongly to the SD sequence as a ratio of 1:1 without dissociation to each subunit from the lateral side, as well as the 30S subunit. The binding constant for 70S increased in the presence of the initiator tRNA, which suggests that the SD and initiator codon of AUG could be also recognized precisely with 70S.
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U2 - 10.1093/nass/nrl025
DO - 10.1093/nass/nrl025
M3 - Article
C2 - 17150811
AN - SCOPUS:36048972537
SN - 1746-8272
SP - 49
EP - 50
JO - Nucleic acids symposium series (2004)
JF - Nucleic acids symposium series (2004)
IS - 50
ER -