Kinetic analysis of ribosome binding process onto mRNA using a quartz-crystal microbalance.

Shuntaro Takahashi, Ryoko Akita, Hiroyuki Furusawa, Yoshihiro Shimizu, Takuya Ueda, Yoshio Okahata

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Translation initiation is the most dynamic and important step along a series of protein synthesis processes. In bacteria, it is generally accepted that the 70S ribosome initially dissociates into the 30S and 50S subunit, and then, the 30S ribosomal subunit binds to the Shine-Dargalno (SD) sequence of mRNA. We analyzed binding kinetics of 70S, 50S and 30S ribosomes to the SD sequence by using a mRNA-immobilized 27 MHz quartz-crystal microbalance (QCM). The 70S ribosome was found to bind strongly to the SD sequence as a ratio of 1:1 without dissociation to each subunit from the lateral side, as well as the 30S subunit. The binding constant for 70S increased in the presence of the initiator tRNA, which suggests that the SD and initiator codon of AUG could be also recognized precisely with 70S.

Original languageEnglish
Pages (from-to)49-50
Number of pages2
JournalNucleic acids symposium series (2004)
Issue number50
Publication statusPublished - 2006 Jan 1
Externally publishedYes

Fingerprint

Quartz Crystal Microbalance Techniques
Ribosomes
Messenger RNA
RNA, Transfer, Met
Ribosome Subunits
Initiator Codon
Bacteria
Proteins

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Takahashi, S., Akita, R., Furusawa, H., Shimizu, Y., Ueda, T., & Okahata, Y. (2006). Kinetic analysis of ribosome binding process onto mRNA using a quartz-crystal microbalance. Nucleic acids symposium series (2004), (50), 49-50.

Kinetic analysis of ribosome binding process onto mRNA using a quartz-crystal microbalance. / Takahashi, Shuntaro; Akita, Ryoko; Furusawa, Hiroyuki; Shimizu, Yoshihiro; Ueda, Takuya; Okahata, Yoshio.

In: Nucleic acids symposium series (2004), No. 50, 01.01.2006, p. 49-50.

Research output: Contribution to journalArticle

Takahashi, S, Akita, R, Furusawa, H, Shimizu, Y, Ueda, T & Okahata, Y 2006, 'Kinetic analysis of ribosome binding process onto mRNA using a quartz-crystal microbalance.', Nucleic acids symposium series (2004), no. 50, pp. 49-50.
Takahashi S, Akita R, Furusawa H, Shimizu Y, Ueda T, Okahata Y. Kinetic analysis of ribosome binding process onto mRNA using a quartz-crystal microbalance. Nucleic acids symposium series (2004). 2006 Jan 1;(50):49-50.
Takahashi, Shuntaro ; Akita, Ryoko ; Furusawa, Hiroyuki ; Shimizu, Yoshihiro ; Ueda, Takuya ; Okahata, Yoshio. / Kinetic analysis of ribosome binding process onto mRNA using a quartz-crystal microbalance. In: Nucleic acids symposium series (2004). 2006 ; No. 50. pp. 49-50.
@article{7eb737c5126743fe95440c1dc9223413,
title = "Kinetic analysis of ribosome binding process onto mRNA using a quartz-crystal microbalance.",
abstract = "Translation initiation is the most dynamic and important step along a series of protein synthesis processes. In bacteria, it is generally accepted that the 70S ribosome initially dissociates into the 30S and 50S subunit, and then, the 30S ribosomal subunit binds to the Shine-Dargalno (SD) sequence of mRNA. We analyzed binding kinetics of 70S, 50S and 30S ribosomes to the SD sequence by using a mRNA-immobilized 27 MHz quartz-crystal microbalance (QCM). The 70S ribosome was found to bind strongly to the SD sequence as a ratio of 1:1 without dissociation to each subunit from the lateral side, as well as the 30S subunit. The binding constant for 70S increased in the presence of the initiator tRNA, which suggests that the SD and initiator codon of AUG could be also recognized precisely with 70S.",
author = "Shuntaro Takahashi and Ryoko Akita and Hiroyuki Furusawa and Yoshihiro Shimizu and Takuya Ueda and Yoshio Okahata",
year = "2006",
month = "1",
day = "1",
language = "English",
pages = "49--50",
journal = "Nucleic acids symposium series (2004)",
issn = "1746-8272",
publisher = "Oxford University Press",
number = "50",

}

TY - JOUR

T1 - Kinetic analysis of ribosome binding process onto mRNA using a quartz-crystal microbalance.

AU - Takahashi, Shuntaro

AU - Akita, Ryoko

AU - Furusawa, Hiroyuki

AU - Shimizu, Yoshihiro

AU - Ueda, Takuya

AU - Okahata, Yoshio

PY - 2006/1/1

Y1 - 2006/1/1

N2 - Translation initiation is the most dynamic and important step along a series of protein synthesis processes. In bacteria, it is generally accepted that the 70S ribosome initially dissociates into the 30S and 50S subunit, and then, the 30S ribosomal subunit binds to the Shine-Dargalno (SD) sequence of mRNA. We analyzed binding kinetics of 70S, 50S and 30S ribosomes to the SD sequence by using a mRNA-immobilized 27 MHz quartz-crystal microbalance (QCM). The 70S ribosome was found to bind strongly to the SD sequence as a ratio of 1:1 without dissociation to each subunit from the lateral side, as well as the 30S subunit. The binding constant for 70S increased in the presence of the initiator tRNA, which suggests that the SD and initiator codon of AUG could be also recognized precisely with 70S.

AB - Translation initiation is the most dynamic and important step along a series of protein synthesis processes. In bacteria, it is generally accepted that the 70S ribosome initially dissociates into the 30S and 50S subunit, and then, the 30S ribosomal subunit binds to the Shine-Dargalno (SD) sequence of mRNA. We analyzed binding kinetics of 70S, 50S and 30S ribosomes to the SD sequence by using a mRNA-immobilized 27 MHz quartz-crystal microbalance (QCM). The 70S ribosome was found to bind strongly to the SD sequence as a ratio of 1:1 without dissociation to each subunit from the lateral side, as well as the 30S subunit. The binding constant for 70S increased in the presence of the initiator tRNA, which suggests that the SD and initiator codon of AUG could be also recognized precisely with 70S.

UR - http://www.scopus.com/inward/record.url?scp=36048972537&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=36048972537&partnerID=8YFLogxK

M3 - Article

C2 - 17150811

AN - SCOPUS:36048972537

SP - 49

EP - 50

JO - Nucleic acids symposium series (2004)

JF - Nucleic acids symposium series (2004)

SN - 1746-8272

IS - 50

ER -