Kinetic analysis of the effects of translation enhancers in translation initiation.

Shuntaro Takahashi; Hiroyuki Furusawa; Yoshihiro Shimizu; Takuya Ueda; Yoshio Okahata

doi:10.1093/nass/nrm023

Kinetic analysis of the effects of translation enhancers in translation initiation.

Shuntaro Takahashi, Hiroyuki Furusawa, Yoshihiro Shimizu, Takuya Ueda, Yoshio Okahata

Research output: Contribution to journal › Article › peer-review

1 Citation (Scopus)

Abstract

Translation initiation is the most important step within a series of protein biosynthesis processes because the incorporation of ribosomes to a mRNA mainly determines efficiencies of translation. In bacteria, translation enhancers located on the 5' upstream of the Shine-Dargalno (SD) sequence on mRNAs are known to accelerate the efficiency of protein biosynthesis. To investigate the role of translation enhancers in translation initiation, we analyzed binding kinetics of a 30S ribosomal subunit to a mRNA immobilized on a 27 MHz quartz-crystal microbalance (QCM). The association constant (Ka) was rather low for the mRNA including a translation enhancer sequence compared with that for the mRNA without translation enhancers. These kinetic parameters suggest that translation enhancers destabilize the ribosome-mRNA complex on an SD sequence to move on the next step of decoding its mRNA.

Original language	English
Pages (from-to)	45-46
Number of pages	2
Journal	Nucleic acids symposium series (2004)
Issue number	51
DOIs	https://doi.org/10.1093/nass/nrm023
Publication status	Published - 2007
Externally published	Yes

ASJC Scopus subject areas

Medicine(all)

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title = "Kinetic analysis of the effects of translation enhancers in translation initiation.",

abstract = "Translation initiation is the most important step within a series of protein biosynthesis processes because the incorporation of ribosomes to a mRNA mainly determines efficiencies of translation. In bacteria, translation enhancers located on the 5' upstream of the Shine-Dargalno (SD) sequence on mRNAs are known to accelerate the efficiency of protein biosynthesis. To investigate the role of translation enhancers in translation initiation, we analyzed binding kinetics of a 30S ribosomal subunit to a mRNA immobilized on a 27 MHz quartz-crystal microbalance (QCM). The association constant (Ka) was rather low for the mRNA including a translation enhancer sequence compared with that for the mRNA without translation enhancers. These kinetic parameters suggest that translation enhancers destabilize the ribosome-mRNA complex on an SD sequence to move on the next step of decoding its mRNA.",

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AU - Furusawa, Hiroyuki

AU - Shimizu, Yoshihiro

AU - Ueda, Takuya

AU - Okahata, Yoshio

N1 - Copyright: This record is sourced from MEDLINE/PubMed, a database of the U.S. National Library of Medicine

PY - 2007

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Kinetic analysis of the effects of translation enhancers in translation initiation.

Abstract

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