Kinetic analysis of the effects of translation enhancers in translation initiation.

Shuntaro Takahashi, Hiroyuki Furusawa, Yoshihiro Shimizu, Takuya Ueda, Yoshio Okahata

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

Translation initiation is the most important step within a series of protein biosynthesis processes because the incorporation of ribosomes to a mRNA mainly determines efficiencies of translation. In bacteria, translation enhancers located on the 5' upstream of the Shine-Dargalno (SD) sequence on mRNAs are known to accelerate the efficiency of protein biosynthesis. To investigate the role of translation enhancers in translation initiation, we analyzed binding kinetics of a 30S ribosomal subunit to a mRNA immobilized on a 27 MHz quartz-crystal microbalance (QCM). The association constant (Ka) was rather low for the mRNA including a translation enhancer sequence compared with that for the mRNA without translation enhancers. These kinetic parameters suggest that translation enhancers destabilize the ribosome-mRNA complex on an SD sequence to move on the next step of decoding its mRNA.

Original languageEnglish
Pages (from-to)45-46
Number of pages2
JournalNucleic acids symposium series (2004)
Issue number51
DOIs
Publication statusPublished - 2007
Externally publishedYes

ASJC Scopus subject areas

  • Medicine(all)

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