Kinetic equivalence of transmembrane ph and electrical potential differences in ATP synthesis

Naoki Soga, Kazuhiko Kinosita, Masasuke Yoshida, Toshiharu Suzuki

    Research output: Contribution to journalArticle

    21 Citations (Scopus)

    Abstract

    ATP synthase is the key player of Mitchell's chemiosmotic theory, converting the energy of transmembrane proton flow into the high energy bond between ADP and phosphate. The proton motive force that drives this reaction consists of two components, the pH difference (ΔpH) across the membrane and transmembrane electrical potential (Δψ). The two are considered thermodynamically equivalent, but kinetic equivalence in the actual ATP synthesis is not warranted, and previous experimental results vary. Here, we show that with the thermophilic Bacillus PS3 ATP synthase that lacks an inhibitory domain of the ε subunit, ΔpH imposed by acid-base transition and Δψ produced by valinomycin-mediated K + diffusion potential contribute equally to the rate of ATP synthesis within the experimental range examined (ΔpH -0.3 to 2.2, Δψ -30 to 140 mV, pH around the catalytic domain 8.0). Either ΔpH or Δψ alone can drive synthesis, even when the other slightly opposes. Δψ was estimated from the Nernst equation, which appeared valid down to 1 mM K + inside the proteoliposomes, due to careful removal of K + from the lipid..

    Original languageEnglish
    Pages (from-to)9633-9639
    Number of pages7
    JournalJournal of Biological Chemistry
    Volume287
    Issue number12
    DOIs
    Publication statusPublished - 2012 Mar 16

    Fingerprint

    Membrane Potentials
    Adenosine Triphosphate
    Kinetics
    Protons
    Valinomycin
    Bacilli
    Adenosine Diphosphate
    Proton-Motive Force
    Phosphates
    Membranes
    Lipids
    Bacillus
    Acids
    Catalytic Domain

    ASJC Scopus subject areas

    • Biochemistry
    • Cell Biology
    • Molecular Biology

    Cite this

    Kinetic equivalence of transmembrane ph and electrical potential differences in ATP synthesis. / Soga, Naoki; Kinosita, Kazuhiko; Yoshida, Masasuke; Suzuki, Toshiharu.

    In: Journal of Biological Chemistry, Vol. 287, No. 12, 16.03.2012, p. 9633-9639.

    Research output: Contribution to journalArticle

    Soga, Naoki ; Kinosita, Kazuhiko ; Yoshida, Masasuke ; Suzuki, Toshiharu. / Kinetic equivalence of transmembrane ph and electrical potential differences in ATP synthesis. In: Journal of Biological Chemistry. 2012 ; Vol. 287, No. 12. pp. 9633-9639.
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