Kinetics of rouleaux formation using TV image analyzer. II. Rat erythrocytes

T. Shiga, K. Imaizumi, N. Maeda, K. Kon

Research output: Contribution to journalArticle

Abstract

With the use of a rheoscope combined with a TV image analyzer, the kinetics of specific pathogen-free rat erythrocyte aggregation was studied. Under certain conditions (γ 7.5 s-1, hematocrit 0.36%, in own plasma, at 25°C) one-dimensional aggregates (rouleaux) were formed without the development of three-dimensional aggregates, perhaps because of very low concentration of γ-globulin. The observed phenomena could be explained by 1) the erythrocyte sedimentation and 2) the rouleaux formation. The time courses, of the biphasic change in erythrocyte count and of the increments in total area and in the area/count, were successfully simulated by a kinetic model of linear polymerization, assuming a sedimentation rate constant and an association rate constant. Further, a Poissonlike distribution of the length of rouleaux was shown, as predicted theoretically on the basis of the same kinetic model.

Original languageEnglish
JournalAmerican Journal of Physiology - Heart and Circulatory Physiology
Volume14
Issue number2
Publication statusPublished - 1983
Externally publishedYes

Fingerprint

Erythrocytes
Erythrocyte Aggregation
Specific Pathogen-Free Organisms
Erythrocyte Count
Blood Sedimentation
Globulins
Hematocrit
Polymerization
Linear Models

ASJC Scopus subject areas

  • Physiology

Cite this

Kinetics of rouleaux formation using TV image analyzer. II. Rat erythrocytes. / Shiga, T.; Imaizumi, K.; Maeda, N.; Kon, K.

In: American Journal of Physiology - Heart and Circulatory Physiology, Vol. 14, No. 2, 1983.

Research output: Contribution to journalArticle

@article{9fd7133caa994b7d9abc9c09b783bc55,
title = "Kinetics of rouleaux formation using TV image analyzer. II. Rat erythrocytes",
abstract = "With the use of a rheoscope combined with a TV image analyzer, the kinetics of specific pathogen-free rat erythrocyte aggregation was studied. Under certain conditions (γ 7.5 s-1, hematocrit 0.36{\%}, in own plasma, at 25°C) one-dimensional aggregates (rouleaux) were formed without the development of three-dimensional aggregates, perhaps because of very low concentration of γ-globulin. The observed phenomena could be explained by 1) the erythrocyte sedimentation and 2) the rouleaux formation. The time courses, of the biphasic change in erythrocyte count and of the increments in total area and in the area/count, were successfully simulated by a kinetic model of linear polymerization, assuming a sedimentation rate constant and an association rate constant. Further, a Poissonlike distribution of the length of rouleaux was shown, as predicted theoretically on the basis of the same kinetic model.",
author = "T. Shiga and K. Imaizumi and N. Maeda and K. Kon",
year = "1983",
language = "English",
volume = "14",
journal = "American Journal of Physiology - Heart and Circulatory Physiology",
issn = "0363-6135",
publisher = "American Physiological Society",
number = "2",

}

TY - JOUR

T1 - Kinetics of rouleaux formation using TV image analyzer. II. Rat erythrocytes

AU - Shiga, T.

AU - Imaizumi, K.

AU - Maeda, N.

AU - Kon, K.

PY - 1983

Y1 - 1983

N2 - With the use of a rheoscope combined with a TV image analyzer, the kinetics of specific pathogen-free rat erythrocyte aggregation was studied. Under certain conditions (γ 7.5 s-1, hematocrit 0.36%, in own plasma, at 25°C) one-dimensional aggregates (rouleaux) were formed without the development of three-dimensional aggregates, perhaps because of very low concentration of γ-globulin. The observed phenomena could be explained by 1) the erythrocyte sedimentation and 2) the rouleaux formation. The time courses, of the biphasic change in erythrocyte count and of the increments in total area and in the area/count, were successfully simulated by a kinetic model of linear polymerization, assuming a sedimentation rate constant and an association rate constant. Further, a Poissonlike distribution of the length of rouleaux was shown, as predicted theoretically on the basis of the same kinetic model.

AB - With the use of a rheoscope combined with a TV image analyzer, the kinetics of specific pathogen-free rat erythrocyte aggregation was studied. Under certain conditions (γ 7.5 s-1, hematocrit 0.36%, in own plasma, at 25°C) one-dimensional aggregates (rouleaux) were formed without the development of three-dimensional aggregates, perhaps because of very low concentration of γ-globulin. The observed phenomena could be explained by 1) the erythrocyte sedimentation and 2) the rouleaux formation. The time courses, of the biphasic change in erythrocyte count and of the increments in total area and in the area/count, were successfully simulated by a kinetic model of linear polymerization, assuming a sedimentation rate constant and an association rate constant. Further, a Poissonlike distribution of the length of rouleaux was shown, as predicted theoretically on the basis of the same kinetic model.

UR - http://www.scopus.com/inward/record.url?scp=0020563908&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0020563908&partnerID=8YFLogxK

M3 - Article

VL - 14

JO - American Journal of Physiology - Heart and Circulatory Physiology

JF - American Journal of Physiology - Heart and Circulatory Physiology

SN - 0363-6135

IS - 2

ER -