Label-free detection of Cu(II) in a human serum sample by using a prion protein-immobilized FET sensor

Shofarul Wustoni; Sho Hideshima; Shigeki Kuroiwa; Takuya Nakanishi; Yasuro Mori; Tetsuya Osaka

doi:10.1039/c5an01115a

Label-free detection of Cu(II) in a human serum sample by using a prion protein-immobilized FET sensor

Shofarul Wustoni, Sho Hideshima, Shigeki Kuroiwa, Takuya Nakanishi, Yasuro Mori, Tetsuya Osaka^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

16 Citations (Scopus)

Abstract

We have developed a field effect transistor (FET) sensor to sensitively detect copper ions (Cu²⁺) in a human serum (HS) sample for promising health-care diagnosis. By utilizing a Cu²⁺-binding prion protein that was immobilized on the FET gate surface, such an FET sensor can provide a simple, label free and highly selective performance, even in HS samples. We demonstrated the sensitivity of the sensor at the nanomolar level, 0-100 nM, which is very useful for the detection range of Cu²⁺ deficiency in practical applications.

Original language	English
Pages (from-to)	6485-6488
Number of pages	4
Journal	Analyst
Volume	140
Issue number	19
DOIs	https://doi.org/10.1039/c5an01115a
Publication status	Published - 2015 Oct 7

ASJC Scopus subject areas

Analytical Chemistry
Biochemistry
Environmental Chemistry
Spectroscopy
Electrochemistry

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10.1039/c5an01115a

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abstract = "We have developed a field effect transistor (FET) sensor to sensitively detect copper ions (Cu2+) in a human serum (HS) sample for promising health-care diagnosis. By utilizing a Cu2+-binding prion protein that was immobilized on the FET gate surface, such an FET sensor can provide a simple, label free and highly selective performance, even in HS samples. We demonstrated the sensitivity of the sensor at the nanomolar level, 0-100 nM, which is very useful for the detection range of Cu2+ deficiency in practical applications.",

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AU - Kuroiwa, Shigeki

AU - Nakanishi, Takuya

AU - Mori, Yasuro

AU - Osaka, Tetsuya

PY - 2015/10/7

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AB - We have developed a field effect transistor (FET) sensor to sensitively detect copper ions (Cu2+) in a human serum (HS) sample for promising health-care diagnosis. By utilizing a Cu2+-binding prion protein that was immobilized on the FET gate surface, such an FET sensor can provide a simple, label free and highly selective performance, even in HS samples. We demonstrated the sensitivity of the sensor at the nanomolar level, 0-100 nM, which is very useful for the detection range of Cu2+ deficiency in practical applications.

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Label-free detection of Cu(II) in a human serum sample by using a prion protein-immobilized FET sensor

Abstract

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