Label-free detection of Cu(II) in a human serum sample by using a prion protein-immobilized FET sensor

Shofarul Wustoni, Sho Hideshima, Shigeki Kuroiwa, Takuya Nakanishi, Yasuro Mori, Tetsuya Osaka

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

We have developed a field effect transistor (FET) sensor to sensitively detect copper ions (Cu<sup>2+</sup>) in a human serum (HS) sample for promising health-care diagnosis. By utilizing a Cu<sup>2+</sup>-binding prion protein that was immobilized on the FET gate surface, such an FET sensor can provide a simple, label free and highly selective performance, even in HS samples. We demonstrated the sensitivity of the sensor at the nanomolar level, 0-100 nM, which is very useful for the detection range of Cu<sup>2+</sup> deficiency in practical applications.

Original languageEnglish
Pages (from-to)6485-6488
Number of pages4
JournalAnalyst
Volume140
Issue number19
DOIs
Publication statusPublished - 2015 Oct 7

Fingerprint

Immobilized Proteins
Field effect transistors
Labels
serum
sensor
protein
Sensors
Serum
Gates (transistor)
Copper
Carrier Proteins
Ions
Delivery of Health Care
Health care
health care
copper
ion
Prions
effect
detection

ASJC Scopus subject areas

  • Analytical Chemistry
  • Spectroscopy
  • Electrochemistry
  • Biochemistry
  • Environmental Chemistry

Cite this

Label-free detection of Cu(II) in a human serum sample by using a prion protein-immobilized FET sensor. / Wustoni, Shofarul; Hideshima, Sho; Kuroiwa, Shigeki; Nakanishi, Takuya; Mori, Yasuro; Osaka, Tetsuya.

In: Analyst, Vol. 140, No. 19, 07.10.2015, p. 6485-6488.

Research output: Contribution to journalArticle

Wustoni, Shofarul ; Hideshima, Sho ; Kuroiwa, Shigeki ; Nakanishi, Takuya ; Mori, Yasuro ; Osaka, Tetsuya. / Label-free detection of Cu(II) in a human serum sample by using a prion protein-immobilized FET sensor. In: Analyst. 2015 ; Vol. 140, No. 19. pp. 6485-6488.
@article{02daf5c2fd364bb9be5fc97aa09f2e88,
title = "Label-free detection of Cu(II) in a human serum sample by using a prion protein-immobilized FET sensor",
abstract = "We have developed a field effect transistor (FET) sensor to sensitively detect copper ions (Cu2+) in a human serum (HS) sample for promising health-care diagnosis. By utilizing a Cu2+-binding prion protein that was immobilized on the FET gate surface, such an FET sensor can provide a simple, label free and highly selective performance, even in HS samples. We demonstrated the sensitivity of the sensor at the nanomolar level, 0-100 nM, which is very useful for the detection range of Cu2+ deficiency in practical applications.",
author = "Shofarul Wustoni and Sho Hideshima and Shigeki Kuroiwa and Takuya Nakanishi and Yasuro Mori and Tetsuya Osaka",
year = "2015",
month = "10",
day = "7",
doi = "10.1039/c5an01115a",
language = "English",
volume = "140",
pages = "6485--6488",
journal = "The Analyst",
issn = "0003-2654",
publisher = "Royal Society of Chemistry",
number = "19",

}

TY - JOUR

T1 - Label-free detection of Cu(II) in a human serum sample by using a prion protein-immobilized FET sensor

AU - Wustoni, Shofarul

AU - Hideshima, Sho

AU - Kuroiwa, Shigeki

AU - Nakanishi, Takuya

AU - Mori, Yasuro

AU - Osaka, Tetsuya

PY - 2015/10/7

Y1 - 2015/10/7

N2 - We have developed a field effect transistor (FET) sensor to sensitively detect copper ions (Cu2+) in a human serum (HS) sample for promising health-care diagnosis. By utilizing a Cu2+-binding prion protein that was immobilized on the FET gate surface, such an FET sensor can provide a simple, label free and highly selective performance, even in HS samples. We demonstrated the sensitivity of the sensor at the nanomolar level, 0-100 nM, which is very useful for the detection range of Cu2+ deficiency in practical applications.

AB - We have developed a field effect transistor (FET) sensor to sensitively detect copper ions (Cu2+) in a human serum (HS) sample for promising health-care diagnosis. By utilizing a Cu2+-binding prion protein that was immobilized on the FET gate surface, such an FET sensor can provide a simple, label free and highly selective performance, even in HS samples. We demonstrated the sensitivity of the sensor at the nanomolar level, 0-100 nM, which is very useful for the detection range of Cu2+ deficiency in practical applications.

UR - http://www.scopus.com/inward/record.url?scp=84941654110&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84941654110&partnerID=8YFLogxK

U2 - 10.1039/c5an01115a

DO - 10.1039/c5an01115a

M3 - Article

VL - 140

SP - 6485

EP - 6488

JO - The Analyst

JF - The Analyst

SN - 0003-2654

IS - 19

ER -