Label-free detection of tumor markers using field effect transistor (FET)-based biosensors for lung cancer diagnosis

Shanshan Cheng, Sho Hideshima, Shigeki Kuroiwa, Takuya Nakanishi, Tetsuya Osaka

Research output: Contribution to journalArticlepeer-review

65 Citations (Scopus)

Abstract

Detection of tumor markers is important for cancer diagnosis. Field effect transistor (FET) has been recognized as a powerful technique for label-free, sensitive, real-time, and multifunctional biosensing. Here, we developed FET biosensors that allow the label-free detection of cytokeratin fragment 21-1 (CYFRA 21-1) and neuron-specific enolase (NSE), useful tumor markers for lung cancer type differentiation. It was found that the FET biosensor was capable of quantitatively detecting these tumor markers in both phosphate-buffered saline and human serum. Additionally, we developed a multianalyte FET biosensor for the selective multiplexed detection of CYFRA 21-1 and NSE at the same time, by integrating two antibody types on the same chip, providing a step towards the realization of sensor arrays. The multianalyte FET biosensor, as described herein, will help for lung cancer differential diagnosis with advantages of simple and rapid detection procedures, low sample consumption, and low cost.

Original languageEnglish
Pages (from-to)329-334
Number of pages6
JournalSensors and Actuators, B: Chemical
Volume212
DOIs
Publication statusPublished - 2015 Jun

Keywords

  • Field effect transistor
  • Lung cancer differential diagnosis
  • Multianalyte biosensor
  • Tumor marker

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Instrumentation
  • Condensed Matter Physics
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Electrical and Electronic Engineering
  • Materials Chemistry

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