Lactobacillus equicursoris sp. nov., isolated from the faeces of a thoroughbred racehorse

Hidetoshi Morita, Mitsuharu Shimazu, Hiroshi Shiono, Hidehiro Toh, Fumihiko Nakajima, Hiroaki Akita, Misako Takagi, Hideto Takami, Masaru Murakami, Toshio Masaoka, Soichi Tanabe, Masahira Hattori

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

We previously isolated five strains of putative lactobacilli from the faeces of a thoroughbred horse (a 4-year-old male). Of the five strains, four were identified as members of existing Lactobacillus species; however, sequence analysis of the 16S rRNA gene revealed that the fifth isolate, DI70T, showed approximately 97% identity (1325/1366 bp) with the type strain of Lactobacillus delbrueckii. Therefore, we considered the possibility that DI70T represents a novel species of the genus Lactobacillus. Cells of strain DI70T were Gram-stain-positive, catalase-negative, non-spore forming, non-motile rods. In phylogenetic trees constructed on the basis of 16S rRNA gene sequences, strain DI70T formed a subcluster in the L. delbrueckii phylogenetic group and was closely related to L. delbrueckii, Lactobacillus crispatus and Lactobacillus jensenii. However, analysis of DNA-DNA relatedness showed that DI70T was genetically distinct from its phylogenetic relatives. The isolate also exhibited distinct biochemical and physiological characteristics when compared with its phylogenetic relatives. It required anaerobic conditions for growth on agar medium. The results indicate that isolate DI70T indeed represents a novel species of the genus Lactobacillus, for which we propose the name Lactobacillus equicursoris sp. nov. The type strain is DI70T (=JCM 14600T =DSM 19284T).

Original languageEnglish
Pages (from-to)109-112
Number of pages4
JournalInternational Journal of Systematic and Evolutionary Microbiology
Volume60
Issue number1
DOIs
Publication statusPublished - 2010
Externally publishedYes

Fingerprint

racehorses
Lactobacillus
Feces
feces
Lactobacillus delbrueckii
phylogenetics
new species
phylogeny
rRNA Genes
DNA
gene
Lactobacillus jensenii
Lactobacillus crispatus
horse
relatedness
ribosomal RNA
agar
anoxic conditions
anaerobic conditions
Catalase

ASJC Scopus subject areas

  • Microbiology
  • Ecology, Evolution, Behavior and Systematics

Cite this

Lactobacillus equicursoris sp. nov., isolated from the faeces of a thoroughbred racehorse. / Morita, Hidetoshi; Shimazu, Mitsuharu; Shiono, Hiroshi; Toh, Hidehiro; Nakajima, Fumihiko; Akita, Hiroaki; Takagi, Misako; Takami, Hideto; Murakami, Masaru; Masaoka, Toshio; Tanabe, Soichi; Hattori, Masahira.

In: International Journal of Systematic and Evolutionary Microbiology, Vol. 60, No. 1, 2010, p. 109-112.

Research output: Contribution to journalArticle

Morita, H, Shimazu, M, Shiono, H, Toh, H, Nakajima, F, Akita, H, Takagi, M, Takami, H, Murakami, M, Masaoka, T, Tanabe, S & Hattori, M 2010, 'Lactobacillus equicursoris sp. nov., isolated from the faeces of a thoroughbred racehorse', International Journal of Systematic and Evolutionary Microbiology, vol. 60, no. 1, pp. 109-112. https://doi.org/10.1099/ijs.0.009290-0
Morita, Hidetoshi ; Shimazu, Mitsuharu ; Shiono, Hiroshi ; Toh, Hidehiro ; Nakajima, Fumihiko ; Akita, Hiroaki ; Takagi, Misako ; Takami, Hideto ; Murakami, Masaru ; Masaoka, Toshio ; Tanabe, Soichi ; Hattori, Masahira. / Lactobacillus equicursoris sp. nov., isolated from the faeces of a thoroughbred racehorse. In: International Journal of Systematic and Evolutionary Microbiology. 2010 ; Vol. 60, No. 1. pp. 109-112.
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