Large scale microfluidic systems for sequential trapping, labeling and content extraction of single cells

Masao Noguchi, Takahiro Arakawa, Keiko Sumitomo, Yoshinori Yamaguchi, Shuichi Shoji

    Research output: Chapter in Book/Report/Conference proceedingConference contribution

    Abstract

    Parallel and sequential manipulation of trapping, labeling and content extraction of single cell was realized using a 2-D simple flow channels. The proposed system consists of main channels, cell trapping pockets, drain channels and single cell content collection channels. We fabricated prototypes having 16 cell trapping pockets and 512 cell trapping pockets. Sequential all single cell handling for COS-1 cell was confirmed in 16 pockets type [1]. The sequential trapping and labeling were achieved in 512 pockets type. Capture rate for COS-1 cell was 83.2% and that for BAT cell was 70.3%. These devices are useful for high throughput single cell analysis.

    Original languageEnglish
    Title of host publicationTRANSDUCERS 2009 - 15th International Conference on Solid-State Sensors, Actuators and Microsystems
    Pages628-631
    Number of pages4
    DOIs
    Publication statusPublished - 2009
    EventTRANSDUCERS 2009 - 15th International Conference on Solid-State Sensors, Actuators and Microsystems - Denver, CO
    Duration: 2009 Jun 212009 Jun 25

    Other

    OtherTRANSDUCERS 2009 - 15th International Conference on Solid-State Sensors, Actuators and Microsystems
    CityDenver, CO
    Period09/6/2109/6/25

    Fingerprint

    Microfluidics
    Labeling
    Channel flow
    Throughput

    Keywords

    • Single cell content extraction
    • Single cell labeling
    • Single cell manipulation
    • Single cell trapping

    ASJC Scopus subject areas

    • Hardware and Architecture
    • Electrical and Electronic Engineering

    Cite this

    Noguchi, M., Arakawa, T., Sumitomo, K., Yamaguchi, Y., & Shoji, S. (2009). Large scale microfluidic systems for sequential trapping, labeling and content extraction of single cells. In TRANSDUCERS 2009 - 15th International Conference on Solid-State Sensors, Actuators and Microsystems (pp. 628-631). [5285403] https://doi.org/10.1109/SENSOR.2009.5285403

    Large scale microfluidic systems for sequential trapping, labeling and content extraction of single cells. / Noguchi, Masao; Arakawa, Takahiro; Sumitomo, Keiko; Yamaguchi, Yoshinori; Shoji, Shuichi.

    TRANSDUCERS 2009 - 15th International Conference on Solid-State Sensors, Actuators and Microsystems. 2009. p. 628-631 5285403.

    Research output: Chapter in Book/Report/Conference proceedingConference contribution

    Noguchi, M, Arakawa, T, Sumitomo, K, Yamaguchi, Y & Shoji, S 2009, Large scale microfluidic systems for sequential trapping, labeling and content extraction of single cells. in TRANSDUCERS 2009 - 15th International Conference on Solid-State Sensors, Actuators and Microsystems., 5285403, pp. 628-631, TRANSDUCERS 2009 - 15th International Conference on Solid-State Sensors, Actuators and Microsystems, Denver, CO, 09/6/21. https://doi.org/10.1109/SENSOR.2009.5285403
    Noguchi M, Arakawa T, Sumitomo K, Yamaguchi Y, Shoji S. Large scale microfluidic systems for sequential trapping, labeling and content extraction of single cells. In TRANSDUCERS 2009 - 15th International Conference on Solid-State Sensors, Actuators and Microsystems. 2009. p. 628-631. 5285403 https://doi.org/10.1109/SENSOR.2009.5285403
    Noguchi, Masao ; Arakawa, Takahiro ; Sumitomo, Keiko ; Yamaguchi, Yoshinori ; Shoji, Shuichi. / Large scale microfluidic systems for sequential trapping, labeling and content extraction of single cells. TRANSDUCERS 2009 - 15th International Conference on Solid-State Sensors, Actuators and Microsystems. 2009. pp. 628-631
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