Lateral diffusion of inositol 1,4,5-trisphosphate receptor type 1 in Purkinje cells is regulated by calcium and actin filaments

Kazumi Fukatsu, Hiroko Bannai, Takafumi Inoue, Katsuhiko Mikoshiba

    Research output: Contribution to journalArticle

    7 Citations (Scopus)

    Abstract

    Inositol 1,4,5-trisphosphate receptor type 1 (IP3R1) is an intracellular Ca2+ release channel that plays crucial roles in the functions of Purkinje cells. The dynamics of IP3R1 on the endoplasmic reticulum membrane and the distribution of IP3R1 in neurons are thought to be important for the spatial regulation of Ca2+ release. In this study, we analyzed the lateral diffusion of IP3R1 in Purkinje cells in cerebellar slice cultures using fluorescence recovery after photobleaching. In the dendrites of Purkinje cells, IP3R1 showed lateral diffusion with an effective diffusion constant of approximately 0.30 μm2/s, and the diffusion of IP3R1 was negatively regulated by actin filaments. We found that actin filaments were also involved in the regulation of IP3R1 diffusion in the spine of Purkinje cells. Glutamate or quisqualic acid stimulation, which activates glutamate receptors and leads to a Ca2+ transient in Purkinje cells, decreased the diffusion of IP3R1 and increased the density of actin in spines. These findings indicate that the neuronal activity-dependent augmentation of actin contributes to the stabilization of IP3R1 in spines.

    Original languageEnglish
    Pages (from-to)1720-1733
    Number of pages14
    JournalJournal of Neurochemistry
    Volume114
    Issue number6
    DOIs
    Publication statusPublished - 2010 Sep

    Fingerprint

    Inositol 1,4,5-Trisphosphate Receptors
    Purkinje Cells
    Actin Cytoskeleton
    Actins
    Calcium
    Spine
    Fluorescence Recovery After Photobleaching
    Quisqualic Acid
    Photobleaching
    Glutamate Receptors
    Dendrites
    Endoplasmic Reticulum
    Neurons
    Glutamic Acid
    Stabilization
    Fluorescence
    Membranes
    Recovery

    Keywords

    • actin filaments
    • dendrite
    • FRAP
    • IPR1
    • Purkinje cells
    • spine

    ASJC Scopus subject areas

    • Biochemistry
    • Cellular and Molecular Neuroscience

    Cite this

    Lateral diffusion of inositol 1,4,5-trisphosphate receptor type 1 in Purkinje cells is regulated by calcium and actin filaments. / Fukatsu, Kazumi; Bannai, Hiroko; Inoue, Takafumi; Mikoshiba, Katsuhiko.

    In: Journal of Neurochemistry, Vol. 114, No. 6, 09.2010, p. 1720-1733.

    Research output: Contribution to journalArticle

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