Layer-controlled hemoglobin vesicles by interaction of hemoglobin with a phospholipid assembly

Shinji Takeoka; Takeru Ohgushi; Kouichiro Terase; Taizen Ohmori; Eishun Tsuchida

Layer-controlled hemoglobin vesicles by interaction of hemoglobin with a phospholipid assembly

Shinji Takeoka, Takeru Ohgushi, Kouichiro Terase, Taizen Ohmori, Eishun Tsuchida^*

^*Corresponding author for this work

School of Advanced Science and Engineering

Research output: Contribution to journal › Article › peer-review

70 Citations (Scopus)

Abstract

Hemoglobin vesicles, which encapsulated concentrated hemoglobin (Hb) with a bilayer of dipalmitoylphosphatidylcholine/cholesterol/palmitic acid, were prepared under various preparation conditions in order to decrease the number of bilayers (n) constructing the vesicle and increase the Hb concentration in the interior of the vesicle ([Hb]_in). n decreased when the surface potential of the bilayer became more negative because of the electrostatic repulsion between the bilayers, while with a changing ζ-potential of Hb from positive to negative, [Hb]_in showed a precipitous fall because of the electrostatic repulsion between Hb and the surface of the bilayer. A temperature decrease leads to a quality increase in the Hb vesicles ([Hb]/[lipid]) in spite of the [Hb]_in decrease by the viscosity increase of the Hb solution. This is explained by the effective reduction of n due to the reduction in membrane fluidity and the protonation of Hb.

Original language	English
Pages (from-to)	1755-1759
Number of pages	5
Journal	Langmuir
Volume	12
Issue number	7
Publication status	Published - 1996 Apr 3

ASJC Scopus subject areas

Materials Science(all)
Condensed Matter Physics
Surfaces and Interfaces
Spectroscopy
Electrochemistry

Cite this

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abstract = "Hemoglobin vesicles, which encapsulated concentrated hemoglobin (Hb) with a bilayer of dipalmitoylphosphatidylcholine/cholesterol/palmitic acid, were prepared under various preparation conditions in order to decrease the number of bilayers (n) constructing the vesicle and increase the Hb concentration in the interior of the vesicle ([Hb]in). n decreased when the surface potential of the bilayer became more negative because of the electrostatic repulsion between the bilayers, while with a changing ζ-potential of Hb from positive to negative, [Hb]in showed a precipitous fall because of the electrostatic repulsion between Hb and the surface of the bilayer. A temperature decrease leads to a quality increase in the Hb vesicles ([Hb]/[lipid]) in spite of the [Hb]in decrease by the viscosity increase of the Hb solution. This is explained by the effective reduction of n due to the reduction in membrane fluidity and the protonation of Hb.",

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T1 - Layer-controlled hemoglobin vesicles by interaction of hemoglobin with a phospholipid assembly

AU - Takeoka, Shinji

AU - Ohgushi, Takeru

AU - Terase, Kouichiro

AU - Ohmori, Taizen

AU - Tsuchida, Eishun

PY - 1996/4/3

Y1 - 1996/4/3

N2 - Hemoglobin vesicles, which encapsulated concentrated hemoglobin (Hb) with a bilayer of dipalmitoylphosphatidylcholine/cholesterol/palmitic acid, were prepared under various preparation conditions in order to decrease the number of bilayers (n) constructing the vesicle and increase the Hb concentration in the interior of the vesicle ([Hb]in). n decreased when the surface potential of the bilayer became more negative because of the electrostatic repulsion between the bilayers, while with a changing ζ-potential of Hb from positive to negative, [Hb]in showed a precipitous fall because of the electrostatic repulsion between Hb and the surface of the bilayer. A temperature decrease leads to a quality increase in the Hb vesicles ([Hb]/[lipid]) in spite of the [Hb]in decrease by the viscosity increase of the Hb solution. This is explained by the effective reduction of n due to the reduction in membrane fluidity and the protonation of Hb.

AB - Hemoglobin vesicles, which encapsulated concentrated hemoglobin (Hb) with a bilayer of dipalmitoylphosphatidylcholine/cholesterol/palmitic acid, were prepared under various preparation conditions in order to decrease the number of bilayers (n) constructing the vesicle and increase the Hb concentration in the interior of the vesicle ([Hb]in). n decreased when the surface potential of the bilayer became more negative because of the electrostatic repulsion between the bilayers, while with a changing ζ-potential of Hb from positive to negative, [Hb]in showed a precipitous fall because of the electrostatic repulsion between Hb and the surface of the bilayer. A temperature decrease leads to a quality increase in the Hb vesicles ([Hb]/[lipid]) in spite of the [Hb]in decrease by the viscosity increase of the Hb solution. This is explained by the effective reduction of n due to the reduction in membrane fluidity and the protonation of Hb.

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Layer-controlled hemoglobin vesicles by interaction of hemoglobin with a phospholipid assembly

Abstract

ASJC Scopus subject areas

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