Layer-controlled hemoglobin vesicles by interaction of hemoglobin with a phospholipid assembly

Shinji Takeoka, Takeru Ohgushi, Kouichiro Terase, Taizen Ohmori, Eishun Tsuchida

    Research output: Contribution to journalArticle

    67 Citations (Scopus)

    Abstract

    Hemoglobin vesicles, which encapsulated concentrated hemoglobin (Hb) with a bilayer of dipalmitoylphosphatidylcholine/cholesterol/palmitic acid, were prepared under various preparation conditions in order to decrease the number of bilayers (n) constructing the vesicle and increase the Hb concentration in the interior of the vesicle ([Hb]in). n decreased when the surface potential of the bilayer became more negative because of the electrostatic repulsion between the bilayers, while with a changing ζ-potential of Hb from positive to negative, [Hb]in showed a precipitous fall because of the electrostatic repulsion between Hb and the surface of the bilayer. A temperature decrease leads to a quality increase in the Hb vesicles ([Hb]/[lipid]) in spite of the [Hb]in decrease by the viscosity increase of the Hb solution. This is explained by the effective reduction of n due to the reduction in membrane fluidity and the protonation of Hb.

    Original languageEnglish
    Pages (from-to)1755-1759
    Number of pages5
    JournalLangmuir
    Volume12
    Issue number7
    Publication statusPublished - 1996 Apr 3

    Fingerprint

    Hemoglobin
    Phospholipids
    hemoglobin
    Hemoglobins
    assembly
    interactions
    Electrostatics
    palmitic acid
    electrostatics
    Palmitic acid
    1,2-Dipalmitoylphosphatidylcholine
    Palmitic Acid
    Fluidity
    Protonation
    Cholesterol
    cholesterol
    Surface potential
    Lipids
    lipids
    Viscosity

    ASJC Scopus subject areas

    • Colloid and Surface Chemistry
    • Physical and Theoretical Chemistry

    Cite this

    Takeoka, S., Ohgushi, T., Terase, K., Ohmori, T., & Tsuchida, E. (1996). Layer-controlled hemoglobin vesicles by interaction of hemoglobin with a phospholipid assembly. Langmuir, 12(7), 1755-1759.

    Layer-controlled hemoglobin vesicles by interaction of hemoglobin with a phospholipid assembly. / Takeoka, Shinji; Ohgushi, Takeru; Terase, Kouichiro; Ohmori, Taizen; Tsuchida, Eishun.

    In: Langmuir, Vol. 12, No. 7, 03.04.1996, p. 1755-1759.

    Research output: Contribution to journalArticle

    Takeoka, S, Ohgushi, T, Terase, K, Ohmori, T & Tsuchida, E 1996, 'Layer-controlled hemoglobin vesicles by interaction of hemoglobin with a phospholipid assembly', Langmuir, vol. 12, no. 7, pp. 1755-1759.
    Takeoka S, Ohgushi T, Terase K, Ohmori T, Tsuchida E. Layer-controlled hemoglobin vesicles by interaction of hemoglobin with a phospholipid assembly. Langmuir. 1996 Apr 3;12(7):1755-1759.
    Takeoka, Shinji ; Ohgushi, Takeru ; Terase, Kouichiro ; Ohmori, Taizen ; Tsuchida, Eishun. / Layer-controlled hemoglobin vesicles by interaction of hemoglobin with a phospholipid assembly. In: Langmuir. 1996 ; Vol. 12, No. 7. pp. 1755-1759.
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