Lcg is a light-inducible and clock-controlled gene expressed in the chicken pineal gland

Megumi Hatori, Toshiyuki Okano, Yoshito Nakajima, Masao Doi, Yoshitaka Fukada

    Research output: Contribution to journalArticle

    5 Citations (Scopus)

    Abstract

    The circadian clock is an autonomous biological clock that is entrainable to environmental 24-h cycles by receiving time cues such as light. Generally, light given at early and late subjective night, respectively, delays and advances the phase of the circadian oscillator. We previously searched for the chicken pineal genes that are induced by light in a phase-dependent manner. The present study undertook cDNA cloning and characterization of a gene whose expression was remarkably up-regulated by light at late subjective night. The mRNA level of this gene exhibited robust diurnal change in the pineal gland, with a peak in the early (subjective) day under light-dark cycles and constant dark condition, and hence it was designated Lcg (Light-inducible and Clock-controlled Gene). Chicken Lcg encodes a coiled-coil protein composed of 560 amino acid residues. Among chicken tissues, the pineal gland and the retina exhibited relatively high expression levels of LCG. LCG was colocalized with γ-tubulin, a centrosomal protein, when expressed in COS7 cells, and LCG is the first example of a clock-related molecule being accumulated at the centrosome. Coimmunoprecipitation of LCG with γ-tubulin in the chicken pineal lysate suggests a link between the circadian oscillator and the centrosomal function.

    Original languageEnglish
    Pages (from-to)1790-1800
    Number of pages11
    JournalJournal of Neurochemistry
    Volume96
    Issue number6
    DOIs
    Publication statusPublished - 2006 Mar

    Fingerprint

    Pineal Gland
    Clocks
    Chickens
    Genes
    Light
    Tubulin
    Biological Clocks
    Centrosome
    Circadian Clocks
    Photoperiod
    Cloning
    Cues
    Retina
    Organism Cloning
    Gene expression
    Proteins
    Complementary DNA
    Gene Expression
    Amino Acids
    Messenger RNA

    Keywords

    • Centrosome
    • Circadian rhythm
    • Light-inducible and clock-controlled gene (Lcg)
    • Photic pathway
    • Pineal gland
    • Retina

    ASJC Scopus subject areas

    • Biochemistry
    • Cellular and Molecular Neuroscience

    Cite this

    Lcg is a light-inducible and clock-controlled gene expressed in the chicken pineal gland. / Hatori, Megumi; Okano, Toshiyuki; Nakajima, Yoshito; Doi, Masao; Fukada, Yoshitaka.

    In: Journal of Neurochemistry, Vol. 96, No. 6, 03.2006, p. 1790-1800.

    Research output: Contribution to journalArticle

    Hatori, Megumi ; Okano, Toshiyuki ; Nakajima, Yoshito ; Doi, Masao ; Fukada, Yoshitaka. / Lcg is a light-inducible and clock-controlled gene expressed in the chicken pineal gland. In: Journal of Neurochemistry. 2006 ; Vol. 96, No. 6. pp. 1790-1800.
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