Leisure-time physical activity and DNA damage among Japanese workers

Ryoko Kawakami, Ikuko Kashino, Hiroshi Kasai, Kazuaki Kawai, Yun Shan Li, Akiko Nanri, Mitsuru Higuchi, Tetsuya Mizoue

Research output: Contribution to journalArticle

Abstract

Background It remains unclear whether daily physical activity is associated with DNA damage. This cross-sectional study examined the association between leisure-time physical activity and urinary 8-hydroxydeoxyguanosine (8-OH-dG), a biomarker of oxidative DNA damage, or urinary 7-methylguanine (m 7 Gua), a biomarker of methylating DNA damage. Methods Participants included 501 workers (294 men and 207 women), aged 20–65 years, from municipal offices in Japan. Urinary 8-OH-dG and m 7 Gua were measured using column-switching HPLC. Physical activity was evaluated using a self-reported questionnaire. The associations between leisure-time physical activity and urinary DNA damage markers were assessed by multiple linear regression analysis, with stratification by occupational physical activity. Results After adjusting for covariates, leisure-time physical activity showed a suggestive inverse correlation with urinary 8-OH-dG levels (P for trend = 0.06), and a significant inverse association with urinary m 7 Gua levels (P for trend = 0.03). In analysis stratified by occupation, inverse correlations were observed in sedentary workers (walking < 30 min/day at work: P for trend = 0.06 and = 0.03 for urinary 8-OH-dG and m 7 Gua, respectively), but not in physically active workers (walking 30 min/day at work). In analysis for each intensity of leisure-time physical activity, light-intensity exercise was associated with lower levels of urinary 8-OH-dG (P for trend = 0.03), whereas moderate-to-high-intensity exercise was associated with lower levels of urinary m 7 Gua (P for trend = 0.02). Conclusions Our results suggest that high levels of leisure-time physical activity are associated with decreased levels of DNA damage in individuals with low physical activity at work.

Original languageEnglish
Article numbere0212499
JournalPLoS ONE
Volume14
Issue number2
DOIs
Publication statusPublished - 2019 Feb 1

Fingerprint

Leisure Activities
physical activity
DNA damage
DNA Damage
Exercise
DNA
Biomarkers
Association reactions
walking
biomarkers
exercise
Walking
Linear regression
Regression analysis
8-oxo-7-hydrodeoxyguanosine
hydroxide ion
cross-sectional studies
light intensity
Genetic Markers
Occupations

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Kawakami, R., Kashino, I., Kasai, H., Kawai, K., Li, Y. S., Nanri, A., ... Mizoue, T. (2019). Leisure-time physical activity and DNA damage among Japanese workers. PLoS ONE, 14(2), [e0212499]. https://doi.org/10.1371/journal.pone.0212499

Leisure-time physical activity and DNA damage among Japanese workers. / Kawakami, Ryoko; Kashino, Ikuko; Kasai, Hiroshi; Kawai, Kazuaki; Li, Yun Shan; Nanri, Akiko; Higuchi, Mitsuru; Mizoue, Tetsuya.

In: PLoS ONE, Vol. 14, No. 2, e0212499, 01.02.2019.

Research output: Contribution to journalArticle

Kawakami, R, Kashino, I, Kasai, H, Kawai, K, Li, YS, Nanri, A, Higuchi, M & Mizoue, T 2019, 'Leisure-time physical activity and DNA damage among Japanese workers' PLoS ONE, vol. 14, no. 2, e0212499. https://doi.org/10.1371/journal.pone.0212499
Kawakami, Ryoko ; Kashino, Ikuko ; Kasai, Hiroshi ; Kawai, Kazuaki ; Li, Yun Shan ; Nanri, Akiko ; Higuchi, Mitsuru ; Mizoue, Tetsuya. / Leisure-time physical activity and DNA damage among Japanese workers. In: PLoS ONE. 2019 ; Vol. 14, No. 2.
@article{4f6dc7e9291e4de5abd758f3b2bdbf00,
title = "Leisure-time physical activity and DNA damage among Japanese workers",
abstract = "Background It remains unclear whether daily physical activity is associated with DNA damage. This cross-sectional study examined the association between leisure-time physical activity and urinary 8-hydroxydeoxyguanosine (8-OH-dG), a biomarker of oxidative DNA damage, or urinary 7-methylguanine (m 7 Gua), a biomarker of methylating DNA damage. Methods Participants included 501 workers (294 men and 207 women), aged 20–65 years, from municipal offices in Japan. Urinary 8-OH-dG and m 7 Gua were measured using column-switching HPLC. Physical activity was evaluated using a self-reported questionnaire. The associations between leisure-time physical activity and urinary DNA damage markers were assessed by multiple linear regression analysis, with stratification by occupational physical activity. Results After adjusting for covariates, leisure-time physical activity showed a suggestive inverse correlation with urinary 8-OH-dG levels (P for trend = 0.06), and a significant inverse association with urinary m 7 Gua levels (P for trend = 0.03). In analysis stratified by occupation, inverse correlations were observed in sedentary workers (walking < 30 min/day at work: P for trend = 0.06 and = 0.03 for urinary 8-OH-dG and m 7 Gua, respectively), but not in physically active workers (walking 30 min/day at work). In analysis for each intensity of leisure-time physical activity, light-intensity exercise was associated with lower levels of urinary 8-OH-dG (P for trend = 0.03), whereas moderate-to-high-intensity exercise was associated with lower levels of urinary m 7 Gua (P for trend = 0.02). Conclusions Our results suggest that high levels of leisure-time physical activity are associated with decreased levels of DNA damage in individuals with low physical activity at work.",
author = "Ryoko Kawakami and Ikuko Kashino and Hiroshi Kasai and Kazuaki Kawai and Li, {Yun Shan} and Akiko Nanri and Mitsuru Higuchi and Tetsuya Mizoue",
year = "2019",
month = "2",
day = "1",
doi = "10.1371/journal.pone.0212499",
language = "English",
volume = "14",
journal = "PLoS One",
issn = "1932-6203",
publisher = "Public Library of Science",
number = "2",

}

TY - JOUR

T1 - Leisure-time physical activity and DNA damage among Japanese workers

AU - Kawakami, Ryoko

AU - Kashino, Ikuko

AU - Kasai, Hiroshi

AU - Kawai, Kazuaki

AU - Li, Yun Shan

AU - Nanri, Akiko

AU - Higuchi, Mitsuru

AU - Mizoue, Tetsuya

PY - 2019/2/1

Y1 - 2019/2/1

N2 - Background It remains unclear whether daily physical activity is associated with DNA damage. This cross-sectional study examined the association between leisure-time physical activity and urinary 8-hydroxydeoxyguanosine (8-OH-dG), a biomarker of oxidative DNA damage, or urinary 7-methylguanine (m 7 Gua), a biomarker of methylating DNA damage. Methods Participants included 501 workers (294 men and 207 women), aged 20–65 years, from municipal offices in Japan. Urinary 8-OH-dG and m 7 Gua were measured using column-switching HPLC. Physical activity was evaluated using a self-reported questionnaire. The associations between leisure-time physical activity and urinary DNA damage markers were assessed by multiple linear regression analysis, with stratification by occupational physical activity. Results After adjusting for covariates, leisure-time physical activity showed a suggestive inverse correlation with urinary 8-OH-dG levels (P for trend = 0.06), and a significant inverse association with urinary m 7 Gua levels (P for trend = 0.03). In analysis stratified by occupation, inverse correlations were observed in sedentary workers (walking < 30 min/day at work: P for trend = 0.06 and = 0.03 for urinary 8-OH-dG and m 7 Gua, respectively), but not in physically active workers (walking 30 min/day at work). In analysis for each intensity of leisure-time physical activity, light-intensity exercise was associated with lower levels of urinary 8-OH-dG (P for trend = 0.03), whereas moderate-to-high-intensity exercise was associated with lower levels of urinary m 7 Gua (P for trend = 0.02). Conclusions Our results suggest that high levels of leisure-time physical activity are associated with decreased levels of DNA damage in individuals with low physical activity at work.

AB - Background It remains unclear whether daily physical activity is associated with DNA damage. This cross-sectional study examined the association between leisure-time physical activity and urinary 8-hydroxydeoxyguanosine (8-OH-dG), a biomarker of oxidative DNA damage, or urinary 7-methylguanine (m 7 Gua), a biomarker of methylating DNA damage. Methods Participants included 501 workers (294 men and 207 women), aged 20–65 years, from municipal offices in Japan. Urinary 8-OH-dG and m 7 Gua were measured using column-switching HPLC. Physical activity was evaluated using a self-reported questionnaire. The associations between leisure-time physical activity and urinary DNA damage markers were assessed by multiple linear regression analysis, with stratification by occupational physical activity. Results After adjusting for covariates, leisure-time physical activity showed a suggestive inverse correlation with urinary 8-OH-dG levels (P for trend = 0.06), and a significant inverse association with urinary m 7 Gua levels (P for trend = 0.03). In analysis stratified by occupation, inverse correlations were observed in sedentary workers (walking < 30 min/day at work: P for trend = 0.06 and = 0.03 for urinary 8-OH-dG and m 7 Gua, respectively), but not in physically active workers (walking 30 min/day at work). In analysis for each intensity of leisure-time physical activity, light-intensity exercise was associated with lower levels of urinary 8-OH-dG (P for trend = 0.03), whereas moderate-to-high-intensity exercise was associated with lower levels of urinary m 7 Gua (P for trend = 0.02). Conclusions Our results suggest that high levels of leisure-time physical activity are associated with decreased levels of DNA damage in individuals with low physical activity at work.

UR - http://www.scopus.com/inward/record.url?scp=85061542539&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85061542539&partnerID=8YFLogxK

U2 - 10.1371/journal.pone.0212499

DO - 10.1371/journal.pone.0212499

M3 - Article

VL - 14

JO - PLoS One

JF - PLoS One

SN - 1932-6203

IS - 2

M1 - e0212499

ER -