Light-beating study of the effect of β-actinin on the interaction between F-actin and heavy meromyosin

Tadakazu Maeda, Shin'ichi Ishiwata, Satoru Fujime

Research output: Contribution to journalArticle

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Abstract

The effects of β-actinin on the interaction between F-actin and heavy meromyosin were studied by quasi-elastic scattering of laser light. The main findings are as follows: 1. 1. Molecular flexing of F-actin in solution has been known to be promoted by bound heavy meromyosin. However, this effect of heavy meromyosin disappeared on the addition of β-actinin (10% by wt to F-actin) in the absence of ATP. 2. 2. When β-actinin was added in the presence of suitable amounts of ATP, the above mentioned effect of β-actinin could not be observed after the ATP was hydrolyzed. However, the effect of β-actinin was observable after application of sheer force to the solution. Subsequent addition of ATP did not eliminate the effect of β-actinin again. 3. 3. Even when β-actinin was added in the presence of ATP, if the concentration of ATP was higher than about 4 mM, the effect of β-actinin was observed. Experiments suggested that the system was not sensitive to the final concentration of ADP, but to the initial concentration of Mg-ATP.

Original languageEnglish
Pages (from-to)445-452
Number of pages8
JournalBBA - Protein Structure
Volume336
Issue number2
DOIs
Publication statusPublished - 1974 Feb 14
Externally publishedYes

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Myosin Subfragments
Actinin
Actins
Adenosine Triphosphate
Light
Elastic scattering
Adenosine Diphosphate
Lasers

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Light-beating study of the effect of β-actinin on the interaction between F-actin and heavy meromyosin. / Maeda, Tadakazu; Ishiwata, Shin'ichi; Fujime, Satoru.

In: BBA - Protein Structure, Vol. 336, No. 2, 14.02.1974, p. 445-452.

Research output: Contribution to journalArticle

Maeda, Tadakazu ; Ishiwata, Shin'ichi ; Fujime, Satoru. / Light-beating study of the effect of β-actinin on the interaction between F-actin and heavy meromyosin. In: BBA - Protein Structure. 1974 ; Vol. 336, No. 2. pp. 445-452.
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