Linear and rotary molecular motors

Kazuhiko Kinosita

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

A single molecule of F1-ATPase has been shown to be the smallest rotary motor ever found, with a central rotor of radius ~1 nm turning in a stator barrel of radius ~5 nm. Continuous rotation of the central γ subunit was revealed under an optical microscope by attaching to γ a huge marker, an actin filament. In a separate study, rotation of a sliding actin filament around its axis has been revealed by attaching a small probe, a single fluorescent dye molecule, to the actin filament and detecting the orientation of the fluorophore, and thus of the actin filament, through polarization imaging. The axial rotation was slow compared to the linear sliding, indicating that myosin does not 'walk' along the helical array of actin protomers but 'runs,' skipping many protomers. The two motors above, one rotary and the other linear, represent two extreme cases of the mode of motor operation: in the F1-ATPase the two partners, the rotor and stator, never detach from each other whereas myosin touches actin only occasionally. In considering the mechanisms of these and other molecular motors, distinction between bending and binding is important. The use of huge and small probes as described above should be useful in studies of protein machines in general, as a means of detecting conformational changes in a single protein molecule during function.

Original languageEnglish
Pages (from-to)5-14
Number of pages10
JournalAdvances in Experimental Medicine and Biology
Volume453
Publication statusPublished - 1998
Externally publishedYes

Fingerprint

Actin Cytoskeleton
Actins
Proton-Translocating ATPases
Protein Subunits
Myosins
Stators
Molecules
Touch
Rotors
Fluorescent Dyes
Proteins
Fluorophores
Microscopes
Polarization
Imaging techniques

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Linear and rotary molecular motors. / Kinosita, Kazuhiko.

In: Advances in Experimental Medicine and Biology, Vol. 453, 1998, p. 5-14.

Research output: Contribution to journalArticle

Kinosita, Kazuhiko. / Linear and rotary molecular motors. In: Advances in Experimental Medicine and Biology. 1998 ; Vol. 453. pp. 5-14.
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