Linkers of Cell Polarity and Cell Cycle Regulation in the Fission Yeast Protein Interaction Network

Federico Vaggi, James Dodgson, Archana Bajpai, Anatole Chessel, Ferenc Jordán, Masamitsu Sato, Rafael Edgardo Carazo-Salas, Attila Csikász-Nagy

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

The study of gene and protein interaction networks has improved our understanding of the multiple, systemic levels of regulation found in eukaryotic and prokaryotic organisms. Here we carry out a large-scale analysis of the protein-protein interaction (PPI) network of fission yeast (Schizosaccharomyces pombe) and establish a method to identify 'linker' proteins that bridge diverse cellular processes - integrating Gene Ontology and PPI data with network theory measures. We test the method on a highly characterized subset of the genome consisting of proteins controlling the cell cycle, cell polarity and cytokinesis and identify proteins likely to play a key role in controlling the temporal changes in the localization of the polarity machinery. Experimental inspection of one such factor, the polarity-regulating RNB protein Sts5, confirms the prediction that it has a cell cycle dependent regulation. Detailed bibliographic inspection of other predicted 'linkers' also confirms the predictive power of the method. As the method is robust to network perturbations and can successfully predict linker proteins, it provides a powerful tool to study the interplay between different cellular processes.

Original languageEnglish
Article numbere1002732
JournalPLoS Computational Biology
Volume8
Issue number10
DOIs
Publication statusPublished - 2012 Oct
Externally publishedYes

Fingerprint

Schizosaccharomyces pombe Proteins
Protein Interaction Maps
Cell Polarity
Protein Interaction Networks
Schizosaccharomyces pombe
Cell Cycle
Polarity
Yeast
yeast
cell cycle
Cells
Proteins
Protein
protein
Cell
Protein-protein Interaction
proteins
protein-protein interactions
Inspection
Schizosaccharomyces

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience
  • Ecology
  • Molecular Biology
  • Genetics
  • Ecology, Evolution, Behavior and Systematics
  • Modelling and Simulation
  • Computational Theory and Mathematics

Cite this

Linkers of Cell Polarity and Cell Cycle Regulation in the Fission Yeast Protein Interaction Network. / Vaggi, Federico; Dodgson, James; Bajpai, Archana; Chessel, Anatole; Jordán, Ferenc; Sato, Masamitsu; Carazo-Salas, Rafael Edgardo; Csikász-Nagy, Attila.

In: PLoS Computational Biology, Vol. 8, No. 10, e1002732, 10.2012.

Research output: Contribution to journalArticle

Vaggi, F, Dodgson, J, Bajpai, A, Chessel, A, Jordán, F, Sato, M, Carazo-Salas, RE & Csikász-Nagy, A 2012, 'Linkers of Cell Polarity and Cell Cycle Regulation in the Fission Yeast Protein Interaction Network', PLoS Computational Biology, vol. 8, no. 10, e1002732. https://doi.org/10.1371/journal.pcbi.1002732
Vaggi, Federico ; Dodgson, James ; Bajpai, Archana ; Chessel, Anatole ; Jordán, Ferenc ; Sato, Masamitsu ; Carazo-Salas, Rafael Edgardo ; Csikász-Nagy, Attila. / Linkers of Cell Polarity and Cell Cycle Regulation in the Fission Yeast Protein Interaction Network. In: PLoS Computational Biology. 2012 ; Vol. 8, No. 10.
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