Lipase production by Trichosporon fermentans WU-C12, a newly isolated yeast

Jiacong Chen, Takashi Ishii, Susumu Shimura, Kotaro Kirimura, Shoji Usami

    Research output: Contribution to journalArticle

    26 Citations (Scopus)

    Abstract

    From the soil samples of various locations, 245 strains of microorganisms were isolated by the enrichment culture method using olive oil as a carbon source. Of these microorganisms one deuteromycotinous yeast was the best producer of extracellular lipase, and the strain WU-C12 was identified as Trichosporon fermentans from the morphological and taxonomical properties. When cultivated at 30°C for 4 d in the medium containing 8% (w/v) corn steep and 3% (v/v) olive oil as sources of nitrogen and carbon, T. fermentans WU-C12 produced 126 U/ml of extracellular lipase. When 3% (v/v) tung oil was used instead of 3% (v/v) olive oil, 146 U/ml of the lipase was produced. Although lipase production decreased to 40 U/ml by the addition of 2% (w/v) glucose to the corn steep-olive oil medium, the strain WU-C12 produced 34 U/ml of lipase in the medium containing 2% (w/v) glucose instead of 3% (v/v) olive oil. On the other hand, T. fermentans WU-C12 could grow and produce lipase in the medium containing n-paraffin as a carbon source.

    Original languageEnglish
    Pages (from-to)412-414
    Number of pages3
    JournalJournal of Fermentation and Bioengineering
    Volume73
    Issue number5
    DOIs
    Publication statusPublished - 1992

    Fingerprint

    Trichosporon
    Lipases
    Olive oil
    Lipase
    Yeast
    Yeasts
    Carbon
    Microorganisms
    Zea mays
    Glucose
    Paraffin
    Paraffins
    Nitrogen
    Soil
    Olive Oil
    Soils

    ASJC Scopus subject areas

    • Biotechnology
    • Applied Microbiology and Biotechnology

    Cite this

    Lipase production by Trichosporon fermentans WU-C12, a newly isolated yeast. / Chen, Jiacong; Ishii, Takashi; Shimura, Susumu; Kirimura, Kotaro; Usami, Shoji.

    In: Journal of Fermentation and Bioengineering, Vol. 73, No. 5, 1992, p. 412-414.

    Research output: Contribution to journalArticle

    Chen, Jiacong ; Ishii, Takashi ; Shimura, Susumu ; Kirimura, Kotaro ; Usami, Shoji. / Lipase production by Trichosporon fermentans WU-C12, a newly isolated yeast. In: Journal of Fermentation and Bioengineering. 1992 ; Vol. 73, No. 5. pp. 412-414.
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    AU - Usami, Shoji

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