@article{52502a73281b4362a42094ede6b3d096,
title = "Lipidomic analysis of cells and extracellular vesicles from high-and low-metastatic triple-negative breast cancer",
abstract = "Extracellular vesicles (EVs) are lipid bilayer nanovesicles secreted from almost all cells including cancer. Cancer-derived EVs contribute to cancer progression and malignancy via educating the surrounding normal cells. In breast cancer, epidemiological and experimental observations indicated that lipids are associated with cancer malignancy. However, lipid compositions of breast cancer EVs and their contributions to cancer progression are unexplored. In this study, we performed a widely targeted quantitative lipidomic analysis in cells and EVs derived from high-and low-metastatic triple-negative breast cancer cell lines, using supercritical fluid chromatography fast-scanning triple-quadrupole mass spectrometry. We demonstrated the differential lipid compositions between EVs and cells of their origin, and between high-and low-metastatic cell lines. Further, we demonstrated EVs from highly metastatic breast cancer accumulated unsaturated diacylglycerols (DGs) compared with EVs from lower-metastatic cells, without increasing the amount in cells. The EVs enriched with DGs could activate the protein kinase D signaling pathway in endothelial cells, which can lead to stimulated angiogenesis. Our results indicate that lipids are selectively loaded into breast cancer EVs to support tumor progression.",
keywords = "Angiogenesis, Diacylglycerol, Endothelial cells, Exosomes, Extracellular vesicles, Lipidomics, Protein kinase D",
author = "Nao Nishida-Aoki and Yoshihiro Izumi and Hiroaki Takeda and Masatomo Takahashi and Takahiro Ochiya and Takeshi Bamba",
note = "Funding Information: This study was partly supported by the Japan Agency for Medical Research and Development AMED-CREST Programs 19gm0910010h0204, 19gm0910013h0003, and 19gm1010010s0202 (Y.I., T.B.); a grant from the Japan Science and Technology Agency JST-CREST Program JPMJCR15G4 of the (Y.I., T.B.); Japan Society for the Promotion of Science (JSPS) Grant-in-Aid for JSPS Fellows 269202 (N.N.-A.); JSPS Grant-in-Aid for Scientific Research on Innovative Areas 17H06304 (Y.I., T.B.); JSPS Grant-in-Aid for Scientific Research (B) 18H01800 (T.B.); and JSPS Grant-in-Aid for Scientific Research (C) 19K05167 (Y.I.). This work was also partly performed in the Cooperative Research Project Program of the Medical Institute of Bioregulation, Kyushu University. Funding Information: Funding: This study was partly supported by the Japan Agency for Medical Research and Development AMED-CREST Programs 19gm0910010h0204, 19gm0910013h0003, and 19gm1010010s0202 (Y.I., T.B.); a grant from the Japan Science and Technology Agency JST-CREST Program JPMJCR15G4 of the (Y.I., T.B.); Japan Society for the Promotion of Science (JSPS) Grant-in-Aid for JSPS Fellows 269202 (N.N.-A.); JSPS Grant-in-Aid for Scientific Research on Innovative Areas 17H06304 (Y.I., T.B.); JSPS Grant-in-?id for Scientific Research (B) 18H01800 (T.B.); and JSPS Grant-in-?id for Scientific Research (C) 19K05167 (Y.I.). This work was also partly performed in the Cooperative Research Project Program of the Medical Institute of Bioregulation, Kyushu University. Publisher Copyright: {\textcopyright} 2020 by the authors. Licensee MDPI, Basel, Switzerland.",
year = "2020",
month = feb,
doi = "10.3390/metabo10020067",
language = "English",
volume = "10",
journal = "Metabolites",
issn = "2218-1989",
publisher = "Multidisciplinary Digital Publishing Institute (MDPI)",
number = "2",
}