Liposomal heme as oxygen carrier under semi-physiological conditions orientation study of heme embedded in a phospholipid bilayer by an electrooptical method

Eishun Tsuchida, Hiroyuki Nishide, Mikiya Sekine, Akihiko Yamagishi

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8 Citations (Scopus)

Abstract

The meso-tetra(α,α,α,α(o-pivalamidophenyl))porphinato iron-mono(1-lauryl-2-methylimidazole) complex embedded in the bilayer of dimyristoylphosphatidylcholine (liposomal heme) binds molecular oxygen reversibly at pH 7 and 37°C. Orientation of the iron porphyrin complex in the phospholipid bilayer was studied by electric birefringence and dichroism. It was observed that both the phospholipid bibilayer of liposome and the porphyrin plane are oriented nearly in parallel to the electric field. Therefore the angle between the porphyrin plane and the bilayer is considered to be practically small.

Original languageEnglish
Pages (from-to)274-278
Number of pages5
JournalBBA - Biomembranes
Volume734
Issue number2
DOIs
Publication statusPublished - 1983 Oct 12

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Keywords

  • Electric birefringence
  • Electric dichroism
  • Hemoglobin model
  • Oxygen carrier
  • Phospholipid bilayer
  • Porphyrin orientation

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology

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