Liquid Chromatography Chip with Low-Dispersion and Low-Pressure-Drop Turn Structure Utilizing a Distribution-Controlled Pillar Array

Muneki Isokawa, Katsuya Takatsuki, Yanting Song, Kailing Shih, Kanki Nakanishi, Zhimin Xie, Donghyun Yoon, Tetsushi Sekiguchi, Takashi Funatsu, Shuichi Shoji, Makoto Tsunoda

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

To realize efficient, fast separations on pillar array columns with turns, a novel turn with low-dispersion and low-pressure-drop properties was developed. This "pillar-distribution-controlled" (PDC) turn was designed as a constant-radius turn filled with octagonal pillars that were arranged to control the linear velocity of the mobile phase in the radial direction. After the pillar positions were adjusted by computational fluid dynamics analysis, 27 mm long pillar array columns with two turns were fabricated on a 20 × 20 mm2 silicon glass plate. The PDC turns suppressed the sample dispersion to a similar extent as the previously developed tapered turn, and the pressure drop of the newly designed turn was reduced to ∼1/6 that of the tapered turn. Moreover, the C18-modified pillar array column with the PDC turns showed good bioanalytical applicability; five fluorescently labeled amino acids were separated in only 24 s at a linear velocity of 7.5 mm/s. The developed turn structure offers the advantages of longer pillar array columns with more turns for the fast analysis of complex samples.

Original languageEnglish
Pages (from-to)6485-6491
Number of pages7
JournalAnalytical Chemistry
Volume88
Issue number12
DOIs
Publication statusPublished - 2016 Jun 21

Fingerprint

Liquid chromatography
Pressure drop
Silicon
Dynamic analysis
Computational fluid dynamics
Amino Acids
Glass
Direction compound

ASJC Scopus subject areas

  • Analytical Chemistry

Cite this

Liquid Chromatography Chip with Low-Dispersion and Low-Pressure-Drop Turn Structure Utilizing a Distribution-Controlled Pillar Array. / Isokawa, Muneki; Takatsuki, Katsuya; Song, Yanting; Shih, Kailing; Nakanishi, Kanki; Xie, Zhimin; Yoon, Donghyun; Sekiguchi, Tetsushi; Funatsu, Takashi; Shoji, Shuichi; Tsunoda, Makoto.

In: Analytical Chemistry, Vol. 88, No. 12, 21.06.2016, p. 6485-6491.

Research output: Contribution to journalArticle

Isokawa, Muneki ; Takatsuki, Katsuya ; Song, Yanting ; Shih, Kailing ; Nakanishi, Kanki ; Xie, Zhimin ; Yoon, Donghyun ; Sekiguchi, Tetsushi ; Funatsu, Takashi ; Shoji, Shuichi ; Tsunoda, Makoto. / Liquid Chromatography Chip with Low-Dispersion and Low-Pressure-Drop Turn Structure Utilizing a Distribution-Controlled Pillar Array. In: Analytical Chemistry. 2016 ; Vol. 88, No. 12. pp. 6485-6491.
@article{91d46f13df814b898f92bd207cb87974,
title = "Liquid Chromatography Chip with Low-Dispersion and Low-Pressure-Drop Turn Structure Utilizing a Distribution-Controlled Pillar Array",
abstract = "To realize efficient, fast separations on pillar array columns with turns, a novel turn with low-dispersion and low-pressure-drop properties was developed. This {"}pillar-distribution-controlled{"} (PDC) turn was designed as a constant-radius turn filled with octagonal pillars that were arranged to control the linear velocity of the mobile phase in the radial direction. After the pillar positions were adjusted by computational fluid dynamics analysis, 27 mm long pillar array columns with two turns were fabricated on a 20 × 20 mm2 silicon glass plate. The PDC turns suppressed the sample dispersion to a similar extent as the previously developed tapered turn, and the pressure drop of the newly designed turn was reduced to ∼1/6 that of the tapered turn. Moreover, the C18-modified pillar array column with the PDC turns showed good bioanalytical applicability; five fluorescently labeled amino acids were separated in only 24 s at a linear velocity of 7.5 mm/s. The developed turn structure offers the advantages of longer pillar array columns with more turns for the fast analysis of complex samples.",
author = "Muneki Isokawa and Katsuya Takatsuki and Yanting Song and Kailing Shih and Kanki Nakanishi and Zhimin Xie and Donghyun Yoon and Tetsushi Sekiguchi and Takashi Funatsu and Shuichi Shoji and Makoto Tsunoda",
year = "2016",
month = "6",
day = "21",
doi = "10.1021/acs.analchem.6b01201",
language = "English",
volume = "88",
pages = "6485--6491",
journal = "Analytical Chemistry",
issn = "0003-2700",
publisher = "American Chemical Society",
number = "12",

}

TY - JOUR

T1 - Liquid Chromatography Chip with Low-Dispersion and Low-Pressure-Drop Turn Structure Utilizing a Distribution-Controlled Pillar Array

AU - Isokawa, Muneki

AU - Takatsuki, Katsuya

AU - Song, Yanting

AU - Shih, Kailing

AU - Nakanishi, Kanki

AU - Xie, Zhimin

AU - Yoon, Donghyun

AU - Sekiguchi, Tetsushi

AU - Funatsu, Takashi

AU - Shoji, Shuichi

AU - Tsunoda, Makoto

PY - 2016/6/21

Y1 - 2016/6/21

N2 - To realize efficient, fast separations on pillar array columns with turns, a novel turn with low-dispersion and low-pressure-drop properties was developed. This "pillar-distribution-controlled" (PDC) turn was designed as a constant-radius turn filled with octagonal pillars that were arranged to control the linear velocity of the mobile phase in the radial direction. After the pillar positions were adjusted by computational fluid dynamics analysis, 27 mm long pillar array columns with two turns were fabricated on a 20 × 20 mm2 silicon glass plate. The PDC turns suppressed the sample dispersion to a similar extent as the previously developed tapered turn, and the pressure drop of the newly designed turn was reduced to ∼1/6 that of the tapered turn. Moreover, the C18-modified pillar array column with the PDC turns showed good bioanalytical applicability; five fluorescently labeled amino acids were separated in only 24 s at a linear velocity of 7.5 mm/s. The developed turn structure offers the advantages of longer pillar array columns with more turns for the fast analysis of complex samples.

AB - To realize efficient, fast separations on pillar array columns with turns, a novel turn with low-dispersion and low-pressure-drop properties was developed. This "pillar-distribution-controlled" (PDC) turn was designed as a constant-radius turn filled with octagonal pillars that were arranged to control the linear velocity of the mobile phase in the radial direction. After the pillar positions were adjusted by computational fluid dynamics analysis, 27 mm long pillar array columns with two turns were fabricated on a 20 × 20 mm2 silicon glass plate. The PDC turns suppressed the sample dispersion to a similar extent as the previously developed tapered turn, and the pressure drop of the newly designed turn was reduced to ∼1/6 that of the tapered turn. Moreover, the C18-modified pillar array column with the PDC turns showed good bioanalytical applicability; five fluorescently labeled amino acids were separated in only 24 s at a linear velocity of 7.5 mm/s. The developed turn structure offers the advantages of longer pillar array columns with more turns for the fast analysis of complex samples.

UR - http://www.scopus.com/inward/record.url?scp=84975794199&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84975794199&partnerID=8YFLogxK

U2 - 10.1021/acs.analchem.6b01201

DO - 10.1021/acs.analchem.6b01201

M3 - Article

VL - 88

SP - 6485

EP - 6491

JO - Analytical Chemistry

JF - Analytical Chemistry

SN - 0003-2700

IS - 12

ER -