Live cell-based sensor cells

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Biosensors utilizing live cells have attracted a great deal of attention because of their high specificity and sensitivity to their targets. We established cytotoxic sensor cells that were transfected with GFP plasmids derived from HSP70B' and BTG2 promoters. These sensor cells have significant advantages in both speed and sensitivity for the detection of cytotoxicity. We created a live cell-based sensorchip fabricated with microfluidic channels. Fluorescence in the sensor cells increased in a CdCl2 dose-dependent manner in the microfluidic channels. With this system, cytotoxic reagents can be quantitatively detected in a quick, sensitive, and high-throughput manner. The combination of sensor cells and microfluidic systems will provide an important basis for the development of μ-TAS technology that can be applied to numerous fields such as biomaterials, nanotoxicology, environmental assessment, and drug screening.

Original languageEnglish
Pages (from-to)5911-5915
Number of pages5
JournalBiomaterials
Volume31
Issue number23
DOIs
Publication statusPublished - 2010 Aug 1
Externally publishedYes

Keywords

  • Cell signaling
  • Cell viability
  • Gene expression
  • Genetic engineering

ASJC Scopus subject areas

  • Bioengineering
  • Ceramics and Composites
  • Biophysics
  • Biomaterials
  • Mechanics of Materials

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