Live cells-based cytotoxic sensor chip fabricated in a microfluidic system

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We report here a live cells-based sensorchip fabricated in microfluidic channels in which several laminar flows were achieved. In addition, we established a cytotoxic sensor cell line, which was transfected with a green fluorescence protein (GFP) plasmid derived from the heat shock protein 70B' (HSP70B') promoter, which is induced by cytotoxic reagents [1-4] (Fig. 1,2). The fluorescence in the sensor cells increased in a CdCl2 dose-dependent manner in the microfluidic channels (Fig.3) [5]. In this system, cytotoxic reagents can be quantitatively detected in a quick, sensitive and high- throughput manner. The combination of sensor cells and microfluidic systems will provide an important basis for the development of micro-total analysis systems (μ-TAS) technology, and can be applied to toxicology, environmental assessment and drug screening.

Original languageEnglish
Title of host publicationDigest of Papers - Microprocesses and Nanotechnology 2007; 20th International Microprocesses and Nanotechnology Conference, MNC
Pages508-509
Number of pages2
DOIs
Publication statusPublished - 2007 Dec 1
Externally publishedYes
Events20th International Microprocesses and Nanotechnology Conference, MNC 2007 - Kyoto, Japan
Duration: 2007 Nov 52007 Nov 8

Publication series

NameDigest of Papers - Microprocesses and Nanotechnology 2007; 20th International Microprocesses and Nanotechnology Conference, MNC

Other

Others20th International Microprocesses and Nanotechnology Conference, MNC 2007
CountryJapan
CityKyoto
Period07/11/507/11/8

ASJC Scopus subject areas

  • Hardware and Architecture
  • Electrical and Electronic Engineering

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  • Cite this

    Taniguchi, A. (2007). Live cells-based cytotoxic sensor chip fabricated in a microfluidic system. In Digest of Papers - Microprocesses and Nanotechnology 2007; 20th International Microprocesses and Nanotechnology Conference, MNC (pp. 508-509). [4456327] (Digest of Papers - Microprocesses and Nanotechnology 2007; 20th International Microprocesses and Nanotechnology Conference, MNC). https://doi.org/10.1109/IMNC.2007.4456327