Live cells-based cytotoxic sensor chip fabricated in a microfluidic system

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We report here a live cells-based sensorchip fabricated in microfluidic channels in which several laminar flows were achieved. In addition, we established a cytotoxic sensor cell line, which was transfected with a green fluorescence protein (GFP) plasmid derived from the heat shock protein 70B' (HSP70B') promoter, which is induced by cytotoxic reagents [1-4] (Fig. 1,2). The fluorescence in the sensor cells increased in a CdCl2 dose-dependent manner in the microfluidic channels (Fig.3) [5]. In this system, cytotoxic reagents can be quantitatively detected in a quick, sensitive and high- throughput manner. The combination of sensor cells and microfluidic systems will provide an important basis for the development of micro-total analysis systems (μ-TAS) technology, and can be applied to toxicology, environmental assessment and drug screening.

Original languageEnglish
Title of host publicationDigest of Papers - Microprocesses and Nanotechnology 2007; 20th International Microprocesses and Nanotechnology Conference, MNC
Pages508-509
Number of pages2
DOIs
Publication statusPublished - 2007
Externally publishedYes
Events20th International Microprocesses and Nanotechnology Conference, MNC 2007 - Kyoto, Japan
Duration: 2007 Nov 52007 Nov 8

Other

Others20th International Microprocesses and Nanotechnology Conference, MNC 2007
CountryJapan
CityKyoto
Period07/11/507/11/8

Fingerprint

Microfluidics
Sensors
Fluorescence
Proteins
Laminar flow
Screening
Cells
Throughput

ASJC Scopus subject areas

  • Hardware and Architecture
  • Electrical and Electronic Engineering

Cite this

Taniguchi, A. (2007). Live cells-based cytotoxic sensor chip fabricated in a microfluidic system. In Digest of Papers - Microprocesses and Nanotechnology 2007; 20th International Microprocesses and Nanotechnology Conference, MNC (pp. 508-509). [4456327] https://doi.org/10.1109/IMNC.2007.4456327

Live cells-based cytotoxic sensor chip fabricated in a microfluidic system. / Taniguchi, Akiyoshi.

Digest of Papers - Microprocesses and Nanotechnology 2007; 20th International Microprocesses and Nanotechnology Conference, MNC. 2007. p. 508-509 4456327.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Taniguchi, A 2007, Live cells-based cytotoxic sensor chip fabricated in a microfluidic system. in Digest of Papers - Microprocesses and Nanotechnology 2007; 20th International Microprocesses and Nanotechnology Conference, MNC., 4456327, pp. 508-509, s20th International Microprocesses and Nanotechnology Conference, MNC 2007, Kyoto, Japan, 07/11/5. https://doi.org/10.1109/IMNC.2007.4456327
Taniguchi A. Live cells-based cytotoxic sensor chip fabricated in a microfluidic system. In Digest of Papers - Microprocesses and Nanotechnology 2007; 20th International Microprocesses and Nanotechnology Conference, MNC. 2007. p. 508-509. 4456327 https://doi.org/10.1109/IMNC.2007.4456327
Taniguchi, Akiyoshi. / Live cells-based cytotoxic sensor chip fabricated in a microfluidic system. Digest of Papers - Microprocesses and Nanotechnology 2007; 20th International Microprocesses and Nanotechnology Conference, MNC. 2007. pp. 508-509
@inproceedings{ba8a0b7f3bca411eb8f80c85e513b3aa,
title = "Live cells-based cytotoxic sensor chip fabricated in a microfluidic system",
abstract = "We report here a live cells-based sensorchip fabricated in microfluidic channels in which several laminar flows were achieved. In addition, we established a cytotoxic sensor cell line, which was transfected with a green fluorescence protein (GFP) plasmid derived from the heat shock protein 70B' (HSP70B') promoter, which is induced by cytotoxic reagents [1-4] (Fig. 1,2). The fluorescence in the sensor cells increased in a CdCl2 dose-dependent manner in the microfluidic channels (Fig.3) [5]. In this system, cytotoxic reagents can be quantitatively detected in a quick, sensitive and high- throughput manner. The combination of sensor cells and microfluidic systems will provide an important basis for the development of micro-total analysis systems (μ-TAS) technology, and can be applied to toxicology, environmental assessment and drug screening.",
author = "Akiyoshi Taniguchi",
year = "2007",
doi = "10.1109/IMNC.2007.4456327",
language = "English",
isbn = "4990247248",
pages = "508--509",
booktitle = "Digest of Papers - Microprocesses and Nanotechnology 2007; 20th International Microprocesses and Nanotechnology Conference, MNC",

}

TY - GEN

T1 - Live cells-based cytotoxic sensor chip fabricated in a microfluidic system

AU - Taniguchi, Akiyoshi

PY - 2007

Y1 - 2007

N2 - We report here a live cells-based sensorchip fabricated in microfluidic channels in which several laminar flows were achieved. In addition, we established a cytotoxic sensor cell line, which was transfected with a green fluorescence protein (GFP) plasmid derived from the heat shock protein 70B' (HSP70B') promoter, which is induced by cytotoxic reagents [1-4] (Fig. 1,2). The fluorescence in the sensor cells increased in a CdCl2 dose-dependent manner in the microfluidic channels (Fig.3) [5]. In this system, cytotoxic reagents can be quantitatively detected in a quick, sensitive and high- throughput manner. The combination of sensor cells and microfluidic systems will provide an important basis for the development of micro-total analysis systems (μ-TAS) technology, and can be applied to toxicology, environmental assessment and drug screening.

AB - We report here a live cells-based sensorchip fabricated in microfluidic channels in which several laminar flows were achieved. In addition, we established a cytotoxic sensor cell line, which was transfected with a green fluorescence protein (GFP) plasmid derived from the heat shock protein 70B' (HSP70B') promoter, which is induced by cytotoxic reagents [1-4] (Fig. 1,2). The fluorescence in the sensor cells increased in a CdCl2 dose-dependent manner in the microfluidic channels (Fig.3) [5]. In this system, cytotoxic reagents can be quantitatively detected in a quick, sensitive and high- throughput manner. The combination of sensor cells and microfluidic systems will provide an important basis for the development of micro-total analysis systems (μ-TAS) technology, and can be applied to toxicology, environmental assessment and drug screening.

UR - http://www.scopus.com/inward/record.url?scp=47349085939&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=47349085939&partnerID=8YFLogxK

U2 - 10.1109/IMNC.2007.4456327

DO - 10.1109/IMNC.2007.4456327

M3 - Conference contribution

SN - 4990247248

SN - 9784990247249

SP - 508

EP - 509

BT - Digest of Papers - Microprocesses and Nanotechnology 2007; 20th International Microprocesses and Nanotechnology Conference, MNC

ER -