Live cells-based cytotoxic sensorchip fabricated in a microfluidic system

Ken Ichi Wada, Akiyoshi Taniguchi, Jun Kobayashi, Masayuki Yamato, Teruo Okano

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

We report here a live cells-based sensorchip fabricated in microfluidic channels in which several laminar flows were achieved. In addition, we established a cytotoxic sensor cell line, which was transfected with a green fluorescence protein (GFP) plasmid derived from the heat shock protein 70B′ (HSP70B′) promoter, which is induced by cytotoxic reagents. The fluorescence in the sensor cells increased in a CdCl2 dose-dependent manner in the microfluidic channels. In this system, cytotoxic reagents can be quantitatively detected in a quick, sensitive and high-throughput manner. The combination of sensor cells and microfluidic systems will provide an important basis for the development of micro-total analysis systems (μ-TAS) technology, and can be applied to toxicology, environmental assessment and drug screening.

Original languageEnglish
Pages (from-to)1513-1517
Number of pages5
JournalBiotechnology and Bioengineering
Volume99
Issue number6
DOIs
Publication statusPublished - 2008 Apr 15
Externally publishedYes

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Microfluidics
Sensors
Fluorescence
Ecotoxicology
Proteins
Cadmium Chloride
Preclinical Drug Evaluations
Heat-Shock Proteins
Laminar flow
Screening
Plasmids
Cells
Throughput
Technology
Cell Line
Pharmaceutical Preparations

Keywords

  • GFP
  • HSP70
  • Microfluidics
  • Promoter
  • Sensorchip

ASJC Scopus subject areas

  • Biotechnology
  • Microbiology

Cite this

Live cells-based cytotoxic sensorchip fabricated in a microfluidic system. / Wada, Ken Ichi; Taniguchi, Akiyoshi; Kobayashi, Jun; Yamato, Masayuki; Okano, Teruo.

In: Biotechnology and Bioengineering, Vol. 99, No. 6, 15.04.2008, p. 1513-1517.

Research output: Contribution to journalArticle

Wada, Ken Ichi ; Taniguchi, Akiyoshi ; Kobayashi, Jun ; Yamato, Masayuki ; Okano, Teruo. / Live cells-based cytotoxic sensorchip fabricated in a microfluidic system. In: Biotechnology and Bioengineering. 2008 ; Vol. 99, No. 6. pp. 1513-1517.
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