Live imaging of cell apoptosis using FRET-based bioprobes in MEMS fabricated microculture dish

R. Kajiyama, Yoshikuni Edagawa, M. Suzuki, A. Nakahara, Donghyun Yoon, Tetsushi Sekiguchi, Shuichi Shoji

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We proposed a novel approach for monitoring cellular caspase-3 activities and controlling morphology of a cell under real time condition using fluorescence resonance energy transfer (FRET) based-bioprobes and the MEMS fabricated microculture dish. The FRET-based bioprobes monitoring caspase-3 activities were successfully introduced into the PC12 cells. In addition, the morphology of the bioprobes introduced cell was controlled along the patterned micro grooves. We observed the activation of the protease and the morphology change of the cell simultaneously when the cells were exposed to an apoptosis inducer.

Original languageEnglish
Title of host publication2013 Transducers and Eurosensors XXVII: The 17th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS and EUROSENSORS 2013
Pages2560-2563
Number of pages4
DOIs
Publication statusPublished - 2013
Event2013 17th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS and EUROSENSORS 2013 - Barcelona
Duration: 2013 Jun 162013 Jun 20

Other

Other2013 17th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS and EUROSENSORS 2013
CityBarcelona
Period13/6/1613/6/20

Fingerprint

Cell death
MEMS
Imaging techniques
Monitoring
Chemical activation
Fluorescence Resonance Energy Transfer

Keywords

  • Bioprobe
  • Cell apoptosis
  • FRET
  • Micro pharmacology
  • Neurodegenerative disease

ASJC Scopus subject areas

  • Hardware and Architecture
  • Electrical and Electronic Engineering

Cite this

Kajiyama, R., Edagawa, Y., Suzuki, M., Nakahara, A., Yoon, D., Sekiguchi, T., & Shoji, S. (2013). Live imaging of cell apoptosis using FRET-based bioprobes in MEMS fabricated microculture dish. In 2013 Transducers and Eurosensors XXVII: The 17th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS and EUROSENSORS 2013 (pp. 2560-2563). [6627328] https://doi.org/10.1109/Transducers.2013.6627328

Live imaging of cell apoptosis using FRET-based bioprobes in MEMS fabricated microculture dish. / Kajiyama, R.; Edagawa, Yoshikuni; Suzuki, M.; Nakahara, A.; Yoon, Donghyun; Sekiguchi, Tetsushi; Shoji, Shuichi.

2013 Transducers and Eurosensors XXVII: The 17th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS and EUROSENSORS 2013. 2013. p. 2560-2563 6627328.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Kajiyama, R, Edagawa, Y, Suzuki, M, Nakahara, A, Yoon, D, Sekiguchi, T & Shoji, S 2013, Live imaging of cell apoptosis using FRET-based bioprobes in MEMS fabricated microculture dish. in 2013 Transducers and Eurosensors XXVII: The 17th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS and EUROSENSORS 2013., 6627328, pp. 2560-2563, 2013 17th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS and EUROSENSORS 2013, Barcelona, 13/6/16. https://doi.org/10.1109/Transducers.2013.6627328
Kajiyama R, Edagawa Y, Suzuki M, Nakahara A, Yoon D, Sekiguchi T et al. Live imaging of cell apoptosis using FRET-based bioprobes in MEMS fabricated microculture dish. In 2013 Transducers and Eurosensors XXVII: The 17th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS and EUROSENSORS 2013. 2013. p. 2560-2563. 6627328 https://doi.org/10.1109/Transducers.2013.6627328
Kajiyama, R. ; Edagawa, Yoshikuni ; Suzuki, M. ; Nakahara, A. ; Yoon, Donghyun ; Sekiguchi, Tetsushi ; Shoji, Shuichi. / Live imaging of cell apoptosis using FRET-based bioprobes in MEMS fabricated microculture dish. 2013 Transducers and Eurosensors XXVII: The 17th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS and EUROSENSORS 2013. 2013. pp. 2560-2563
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KW - Micro pharmacology

KW - Neurodegenerative disease

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