Local spectrum measurement system for 5-aminolaevulinic acid induced protoporphyrin IX fluorescence

Takehiro Ando, Masafumi Noguchi, Etsuko Kobayashi, Hongen Liao, Takashi Maruyama, Yoshihiro Muragaki, Hiroshi Iseki, Ichiro Sakuma

    Research output: Chapter in Book/Report/Conference proceedingConference contribution

    1 Citation (Scopus)

    Abstract

    We have developed a local spectrum measurement system for 5-Aminolaevulinic acid (5-ALA) induced Protoporphyrin IX (PpIX) fluorescence, which consists of a semiconductor LASER, a CCD spectrometer, a personal computer and a fluorescence measurement probe. Performance evaluation results showed that the probe has sufficient ability to measure PpIX fluorescence with a spatial resolution of about 0.8mm around the working-distance. We used this probe for measurement of resected brain tissues to evaluate discrimination ability of 5-ALA induced PpIX fluorescence. To compare spectrums with pathology, we used a tissue holder to freeze tissues keeping their shape. This procedure enables precise comparison each measured point with pathology. Thirteen samples of brain tissue were measured using the developed system, and the results showed that PpIX fluorescence could be detected in tumor lesion even less than 1mm in diameter. Although some samples shrank to about 80% of holder size, characterized shape enabled to compare spectrums with pathology.

    Original languageEnglish
    Title of host publicationWorld Congress on Medical Physics and Biomedical Engineering
    Subtitle of host publicationDiagnostic Imaging
    Pages375-378
    Number of pages4
    Edition2
    DOIs
    Publication statusPublished - 2009 Dec 1
    EventWorld Congress on Medical Physics and Biomedical Engineering: Diagnostic Imaging - Munich, Germany
    Duration: 2009 Sep 72009 Sep 12

    Publication series

    NameIFMBE Proceedings
    Number2
    Volume25
    ISSN (Print)1680-0737

    Conference

    ConferenceWorld Congress on Medical Physics and Biomedical Engineering: Diagnostic Imaging
    CountryGermany
    CityMunich
    Period09/9/709/9/12

    Keywords

    • Brain tumor
    • Fluorescence spectrum
    • Protoporphyrin IX

    ASJC Scopus subject areas

    • Bioengineering
    • Biomedical Engineering

    Fingerprint Dive into the research topics of 'Local spectrum measurement system for 5-aminolaevulinic acid induced protoporphyrin IX fluorescence'. Together they form a unique fingerprint.

  • Cite this

    Ando, T., Noguchi, M., Kobayashi, E., Liao, H., Maruyama, T., Muragaki, Y., Iseki, H., & Sakuma, I. (2009). Local spectrum measurement system for 5-aminolaevulinic acid induced protoporphyrin IX fluorescence. In World Congress on Medical Physics and Biomedical Engineering: Diagnostic Imaging (2 ed., pp. 375-378). (IFMBE Proceedings; Vol. 25, No. 2). https://doi.org/10.1007/978-3-642-03879-2-106