Local spectrum measurement system for 5-aminolaevulinic acid induced protoporphyrin IX fluorescence

Takehiro Ando, Masafumi Noguchi, Etsuko Kobayashi, Hongen Liao, Takashi Maruyama, Yoshihiro Muragaki, Hiroshi Iseki, Ichiro Sakuma

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

We have developed a local spectrum measurement system for 5-Aminolaevulinic acid (5-ALA) induced Protoporphyrin IX (PpIX) fluorescence, which consists of a semiconductor LASER, a CCD spectrometer, a personal computer and a fluorescence measurement probe. Performance evaluation results showed that the probe has sufficient ability to measure PpIX fluorescence with a spatial resolution of about 0.8mm around the working-distance. We used this probe for measurement of resected brain tissues to evaluate discrimination ability of 5-ALA induced PpIX fluorescence. To compare spectrums with pathology, we used a tissue holder to freeze tissues keeping their shape. This procedure enables precise comparison each measured point with pathology. Thirteen samples of brain tissue were measured using the developed system, and the results showed that PpIX fluorescence could be detected in tumor lesion even less than 1mm in diameter. Although some samples shrank to about 80% of holder size, characterized shape enabled to compare spectrums with pathology.

Original languageEnglish
Title of host publicationIFMBE Proceedings
Pages375-378
Number of pages4
Volume25
Edition2
DOIs
Publication statusPublished - 2009
Externally publishedYes
EventWorld Congress on Medical Physics and Biomedical Engineering: Diagnostic Imaging - Munich
Duration: 2009 Sep 72009 Sep 12

Other

OtherWorld Congress on Medical Physics and Biomedical Engineering: Diagnostic Imaging
CityMunich
Period09/9/709/9/12

Fingerprint

Aminolevulinic Acid
Fluorescence
Pathology
Acids
Tissue
Brain
Charge coupled devices
Personal computers
Spectrometers
Tumors
protoporphyrin IX
Semiconductor materials

Keywords

  • Brain tumor
  • Fluorescence spectrum
  • Protoporphyrin IX

ASJC Scopus subject areas

  • Biomedical Engineering
  • Bioengineering

Cite this

Ando, T., Noguchi, M., Kobayashi, E., Liao, H., Maruyama, T., Muragaki, Y., ... Sakuma, I. (2009). Local spectrum measurement system for 5-aminolaevulinic acid induced protoporphyrin IX fluorescence. In IFMBE Proceedings (2 ed., Vol. 25, pp. 375-378) https://doi.org/10.1007/978-3-642-03879-2-106

Local spectrum measurement system for 5-aminolaevulinic acid induced protoporphyrin IX fluorescence. / Ando, Takehiro; Noguchi, Masafumi; Kobayashi, Etsuko; Liao, Hongen; Maruyama, Takashi; Muragaki, Yoshihiro; Iseki, Hiroshi; Sakuma, Ichiro.

IFMBE Proceedings. Vol. 25 2. ed. 2009. p. 375-378.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Ando, T, Noguchi, M, Kobayashi, E, Liao, H, Maruyama, T, Muragaki, Y, Iseki, H & Sakuma, I 2009, Local spectrum measurement system for 5-aminolaevulinic acid induced protoporphyrin IX fluorescence. in IFMBE Proceedings. 2 edn, vol. 25, pp. 375-378, World Congress on Medical Physics and Biomedical Engineering: Diagnostic Imaging, Munich, 09/9/7. https://doi.org/10.1007/978-3-642-03879-2-106
Ando T, Noguchi M, Kobayashi E, Liao H, Maruyama T, Muragaki Y et al. Local spectrum measurement system for 5-aminolaevulinic acid induced protoporphyrin IX fluorescence. In IFMBE Proceedings. 2 ed. Vol. 25. 2009. p. 375-378 https://doi.org/10.1007/978-3-642-03879-2-106
Ando, Takehiro ; Noguchi, Masafumi ; Kobayashi, Etsuko ; Liao, Hongen ; Maruyama, Takashi ; Muragaki, Yoshihiro ; Iseki, Hiroshi ; Sakuma, Ichiro. / Local spectrum measurement system for 5-aminolaevulinic acid induced protoporphyrin IX fluorescence. IFMBE Proceedings. Vol. 25 2. ed. 2009. pp. 375-378
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