Localization of orexin-A-like immunoreactivity in prolactin cells in the bullfrog (Rana catesbeiana) pituitary

Toshiharu Yamamoto, Hirohumi Suzuki, Haruko Uemura, Kazutoshi Yamamoto, Sakaé Kikuyama

    Research output: Contribution to journalArticle

    23 Citations (Scopus)

    Abstract

    Immunohistochemical techniques were employed to investigate the distribution of orexin-A-like immunoreactivity in the bullfrog (Rana catesbeiana) pituitary. Orexin-A-immunoreactive cells were scattered throughout the pars distalis. We found that these cells corresponded to the cells immunostained with antiserum against bullfrog prolactin (fPRL). Immunoelectron microscopic analysis indicated that an orexin-A-like substance coexisted with fPRL within secretory granules. Western blot analysis of bullfrog pituitary extract revealed that anti-human orexin-A antiserum labeled two separate bands which were not labeled with anti-fPRL antiserum. The present study has, for the first time, provided evidence of the intragranular colocalization of orexin-A-like and PRL immunoreactivities in the bullfrog pituitary.

    Original languageEnglish
    Pages (from-to)186-192
    Number of pages7
    JournalGeneral and Comparative Endocrinology
    Volume135
    Issue number2
    DOIs
    Publication statusPublished - 2004 Jan 15

    Fingerprint

    Rana catesbeiana
    Lithobates catesbeianus
    prolactin
    Prolactin
    antiserum
    Immune Sera
    secretory granules
    cells
    Western blotting
    Secretory Vesicles
    extracts
    Western Blotting
    Orexins
    methodology

    Keywords

    • Colocalization
    • Frog
    • Immunocytochemistry
    • Orexin-A
    • Pituitary
    • Prolactin

    ASJC Scopus subject areas

    • Endocrinology

    Cite this

    Localization of orexin-A-like immunoreactivity in prolactin cells in the bullfrog (Rana catesbeiana) pituitary. / Yamamoto, Toshiharu; Suzuki, Hirohumi; Uemura, Haruko; Yamamoto, Kazutoshi; Kikuyama, Sakaé.

    In: General and Comparative Endocrinology, Vol. 135, No. 2, 15.01.2004, p. 186-192.

    Research output: Contribution to journalArticle

    Yamamoto, Toshiharu ; Suzuki, Hirohumi ; Uemura, Haruko ; Yamamoto, Kazutoshi ; Kikuyama, Sakaé. / Localization of orexin-A-like immunoreactivity in prolactin cells in the bullfrog (Rana catesbeiana) pituitary. In: General and Comparative Endocrinology. 2004 ; Vol. 135, No. 2. pp. 186-192.
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    abstract = "Immunohistochemical techniques were employed to investigate the distribution of orexin-A-like immunoreactivity in the bullfrog (Rana catesbeiana) pituitary. Orexin-A-immunoreactive cells were scattered throughout the pars distalis. We found that these cells corresponded to the cells immunostained with antiserum against bullfrog prolactin (fPRL). Immunoelectron microscopic analysis indicated that an orexin-A-like substance coexisted with fPRL within secretory granules. Western blot analysis of bullfrog pituitary extract revealed that anti-human orexin-A antiserum labeled two separate bands which were not labeled with anti-fPRL antiserum. The present study has, for the first time, provided evidence of the intragranular colocalization of orexin-A-like and PRL immunoreactivities in the bullfrog pituitary.",
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    AU - Yamamoto, Kazutoshi

    AU - Kikuyama, Sakaé

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