Localization of prolactin receptor in the newt brain

Itaru Hasunuma, Fumiyo Toyoda, Kazutoshi Yamamoto, Masayuki Yamashita, Sakae Kikuyama

    Research output: Contribution to journalArticle

    15 Citations (Scopus)

    Abstract

    In the male newt Cynops pyrrhogaster, prolactin (PRL) acts directly on the central nervous system and induces courtship behavior. As a step to elucidate the localization of neurons on which PRL acts, we developed a polyclonal antibody against an oligopeptide having a sequence completely identical with a part of the sequence of PRL receptors (PRLRs) of two species of newts, C. pyrrhogaster and C. ensicauda, and performed an immunohistochemical study with this antibody. PRLR-immunoreactive cells were observed in the medial amygdala, anterior preoptic area, magnocellular preoptic nucleus, suprachiasmatic nucleus, nucleus of the periventricular organ, ventral hypothalamic nucleus, and choroid plexus. We also performed in situ hybridization with a 35S-labeled newt PRLR antisense RNA probe and detected signals in the preoptic area and choroid plexus. Colocalization of both PRLR-like immunoreactivity and arginine vasotocin-like or mesotocin-like immunoreactivity was demonstrated in the magnocellular preoptic nucleus. This is the first report of PRLR localization in the amphibian brain.

    Original languageEnglish
    Pages (from-to)477-485
    Number of pages9
    JournalCell and Tissue Research
    Volume320
    Issue number3
    DOIs
    Publication statusPublished - 2005 Jun

    Fingerprint

    Prolactin Receptors
    Salamandridae
    Preoptic Area
    Brain
    Choroid Plexus
    Prolactin
    Vasotocin
    RNA Probes
    Courtship
    Antisense RNA
    Oligopeptides
    Suprachiasmatic Nucleus
    Antibodies
    Neurology
    Amphibians
    Amygdala
    Neurons
    In Situ Hybridization
    Central Nervous System

    Keywords

    • Arginine vasotocin
    • Brain
    • C. ensicauda (Urodela)
    • Mesotocin
    • Newts, Cynops pyrrhogaster
    • Prolactin receptor

    ASJC Scopus subject areas

    • Anatomy
    • Clinical Biochemistry
    • Cell Biology

    Cite this

    Hasunuma, I., Toyoda, F., Yamamoto, K., Yamashita, M., & Kikuyama, S. (2005). Localization of prolactin receptor in the newt brain. Cell and Tissue Research, 320(3), 477-485. https://doi.org/10.1007/s00441-004-1041-0

    Localization of prolactin receptor in the newt brain. / Hasunuma, Itaru; Toyoda, Fumiyo; Yamamoto, Kazutoshi; Yamashita, Masayuki; Kikuyama, Sakae.

    In: Cell and Tissue Research, Vol. 320, No. 3, 06.2005, p. 477-485.

    Research output: Contribution to journalArticle

    Hasunuma, I, Toyoda, F, Yamamoto, K, Yamashita, M & Kikuyama, S 2005, 'Localization of prolactin receptor in the newt brain', Cell and Tissue Research, vol. 320, no. 3, pp. 477-485. https://doi.org/10.1007/s00441-004-1041-0
    Hasunuma I, Toyoda F, Yamamoto K, Yamashita M, Kikuyama S. Localization of prolactin receptor in the newt brain. Cell and Tissue Research. 2005 Jun;320(3):477-485. https://doi.org/10.1007/s00441-004-1041-0
    Hasunuma, Itaru ; Toyoda, Fumiyo ; Yamamoto, Kazutoshi ; Yamashita, Masayuki ; Kikuyama, Sakae. / Localization of prolactin receptor in the newt brain. In: Cell and Tissue Research. 2005 ; Vol. 320, No. 3. pp. 477-485.
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