Localized expression of heat shock protein in cell population by micro heater device

R. Ueno, F. Yesilköy, J. Brugger, Akiyoshi Taniguchi, Y. Sakai, B. J. Kim

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

In this paper, we demonstrate the micro heater device integrated with a micro chamber for investigation on cell-cell interactions under local thermal stimulation. The temperature distribution in the micro chamber was confirmed by on-chip temperature sensors and finite element method simulation. We successfully stimulated NIH-3T3 cells by Joule heating through the micro heater and observed localized expression of heat shock proteins (HSPs) after cells were cultured in the micro chamber confluently. The temperature distribution and the localized expression area consisted. Thus, the thermal stimulation area was confined in the micro chamber. Besides, localized expression of HSPs was observed in a cell monolayer. This device will be widely applied for the study on cell-cell interactions under local stimulation.

Original languageEnglish
Title of host publication18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014
PublisherChemical and Biological Microsystems Society
Pages645-647
Number of pages3
ISBN (Print)9780979806476
Publication statusPublished - 2014
Externally publishedYes
Event18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014 - San Antonio, United States
Duration: 2014 Oct 262014 Oct 30

Other

Other18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014
CountryUnited States
CitySan Antonio
Period14/10/2614/10/30

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Keywords

  • Cell-cell interaction
  • Finite element method simulation
  • Local thermal stimulation

ASJC Scopus subject areas

  • Control and Systems Engineering

Cite this

Ueno, R., Yesilköy, F., Brugger, J., Taniguchi, A., Sakai, Y., & Kim, B. J. (2014). Localized expression of heat shock protein in cell population by micro heater device. In 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014 (pp. 645-647). Chemical and Biological Microsystems Society.