Logic gate using artificial cell-membrane: NAND operation by transmembrane DNA via a biological nanopore

Hiroki Yasuga, Ryuji Kawano, Masahiro Takinoue, Yutaro Tsuji, Toshihisa Osaki, Koki Kamiya, Norihisa Miki, Shoji Takeuchi

Research output: Chapter in Book/Report/Conference proceedingConference contribution

3 Citations (Scopus)

Abstract

This paper describes microfluidic logic gates which use DNA and biological nanopores. Single-stranded DNA (ssDNA) can pass through αHL, a biological nanopore, incorporated in bilayer lipid membranes (BLMs), whereas double-stranded DNA (dsDNA) cannot. In this study, these passing and non-passing phenomena were applied as the binary system and logic gates. Two types of ssDNA were used as inputs, while the output was obtained by electrical signals across the nanopores, which recognizes whether ssDNA passed through the nanopores or not. NAND gate was successfully demonstrated by exploiting the mechanism. The proposed approach herein is significantly different from the conventional computation using DNA in the respect that electrical signals are directly obtained as the output, which drastically facilities the microfluidic system to connect to electrical systems for fast and accurate computing. In addition, it is not required to use fluorescence, enzyme or PCR in order to obtain outputs. We believe that this method leads to a rapid computing system using biomolecules.

Original languageEnglish
Title of host publicationIEEE 26th International Conference on Micro Electro Mechanical Systems, MEMS 2013
Pages1005-1006
Number of pages2
DOIs
Publication statusPublished - 2013 Apr 2
Externally publishedYes
EventIEEE 26th International Conference on Micro Electro Mechanical Systems, MEMS 2013 - Taipei, Taiwan, Province of China
Duration: 2013 Jan 202013 Jan 24

Publication series

NameProceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)
ISSN (Print)1084-6999

Conference

ConferenceIEEE 26th International Conference on Micro Electro Mechanical Systems, MEMS 2013
CountryTaiwan, Province of China
CityTaipei
Period13/1/2013/1/24

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ASJC Scopus subject areas

  • Electrical and Electronic Engineering
  • Mechanical Engineering
  • Condensed Matter Physics
  • Electronic, Optical and Magnetic Materials

Cite this

Yasuga, H., Kawano, R., Takinoue, M., Tsuji, Y., Osaki, T., Kamiya, K., Miki, N., & Takeuchi, S. (2013). Logic gate using artificial cell-membrane: NAND operation by transmembrane DNA via a biological nanopore. In IEEE 26th International Conference on Micro Electro Mechanical Systems, MEMS 2013 (pp. 1005-1006). [6474417] (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)). https://doi.org/10.1109/MEMSYS.2013.6474417