Long-term monitoring of the succession of a microbial community in activated sludge from a circulation flush toilet as a closed system

Tatsuhiko Hoshino, Takeshi Terahara, Kenji Yamada, Hideo Okuda, Isao Suzuki, Satoshi Tsuneda, Akira Hirata, Yuhei Inamori

    Research output: Contribution to journalArticle

    15 Citations (Scopus)

    Abstract

    The microbial diversity and community succession of a circulation flush toilet were investigated by terminal restriction fragment length polymorphism and cloning analyses. Clonal libraries of 16S rRNA gene on day 3 and day 127 were constructed. On day 3, 102 clones were sequenced; Proteobacteria and Bacteroidetes accounted for 27% and 45%, respectively. On day 127, Proteobacteria had increased to 43% and Bacteroidetes had decreased to 26% of a total of 100 clones. Terminal restriction fragment length polymorphism peaks were identified by in silico analysis of clone libraries. The relative abundances of Nitrosomonas increased from 1% to 6% with commencement of nitrification and denitrification. Similarly, the relative abundance of terminal restriction fragments generated from Xanthomonas increased from 3% to 10%. Therefore, these bacteria could play a prominent role in this process. To reveal the relationship between stability of the microbial community and performance of the system, microbial community succession was visualized by multidimensional scaling analysis. The microbial community structure changed markedly, particularly during the start-up period of the system. The plots then became stable after the start of nitrification and denitrification. This result suggests that the succession of microbial community structure had a correlation with the performance of the system.

    Original languageEnglish
    Pages (from-to)459-470
    Number of pages12
    JournalFEMS Microbiology Ecology
    Volume55
    Issue number3
    DOIs
    Publication statusPublished - 2006 Mar

    Fingerprint

    Sewage
    Bacteroidetes
    activated sludge
    Nitrification
    Denitrification
    microbial community
    Proteobacteria
    Clone Cells
    clone
    Restriction Fragment Length Polymorphisms
    Nitrosomonas
    monitoring
    Xanthomonas
    nitrification
    denitrification
    relative abundance
    polymorphism
    community structure
    rRNA Genes
    Computer Simulation

    Keywords

    • Multidimensional scaling analysis
    • Population dynamics
    • Terminal restriction fragment length polymorphism
    • Wastewater treatment

    ASJC Scopus subject areas

    • Ecology
    • Applied Microbiology and Biotechnology
    • Microbiology

    Cite this

    Long-term monitoring of the succession of a microbial community in activated sludge from a circulation flush toilet as a closed system. / Hoshino, Tatsuhiko; Terahara, Takeshi; Yamada, Kenji; Okuda, Hideo; Suzuki, Isao; Tsuneda, Satoshi; Hirata, Akira; Inamori, Yuhei.

    In: FEMS Microbiology Ecology, Vol. 55, No. 3, 03.2006, p. 459-470.

    Research output: Contribution to journalArticle

    Hoshino, Tatsuhiko ; Terahara, Takeshi ; Yamada, Kenji ; Okuda, Hideo ; Suzuki, Isao ; Tsuneda, Satoshi ; Hirata, Akira ; Inamori, Yuhei. / Long-term monitoring of the succession of a microbial community in activated sludge from a circulation flush toilet as a closed system. In: FEMS Microbiology Ecology. 2006 ; Vol. 55, No. 3. pp. 459-470.
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